【摘要】：目的:比較觀察年輕小鼠和自然衰老小鼠的骨質(zhì)特征及骨髓間充質(zhì)干細胞(bonemarrowmesenchymal stem cells,BMMSCs)的生物學功能。方法:分別取12月齡和2月齡C57BL/6J小鼠,Micro-CT活體掃描兩組小鼠股骨毉骺端,三維圖像重建后觀察其骨質(zhì)特征;體外分離、培養(yǎng)兩組小鼠的BMMSCs,通過MTT、克隆形成和成骨誘導觀察其增殖、自我更新和成骨分化能力;并采用實時定量基因擴增熒光檢測系統(tǒng)(Real-time PCR)對成骨誘導14 d后的BMMSCs重要相關(guān)基因OCN進行檢測。結(jié)果:與2月齡小鼠相比,12月齡小鼠出現(xiàn)了骨衰老癥狀,主要表現(xiàn)為骨密度及骨小梁強度下降;BMMSCs的增殖、自我更新和成骨分化能力以及OCN mRNA表達水平均明顯降低。結(jié)論:BMMSCs可能在骨衰老中發(fā)揮了重要作用。
[Abstract]:Aim: to observe the characteristics of bone and the biological function of bone marrow mesenchymal stem cells (bonemarrowmesenchymal stem cells,BMMSCs) in young mice and natural aging mice. Methods: 12-month-old and 2-month-old C57BL/6J mice were used to scan the epiphysis of femur with Micro-CT in vivo. The bone characteristics were observed after three-dimensional image reconstruction. The proliferation, self-renewal and osteogenic differentiation of BMMSCs, in two groups of mice were observed by MTT, clone formation and osteogenesis induction in vitro. Real-time quantitative gene amplification fluorescence detection system (Real-time PCR) was used to detect the important BMMSCs-related gene OCN 14 days after osteogenic induction. Results: compared with the 2-month-old mice, 12-month-old mice had bone senescence symptoms, the main manifestations were decreased bone mineral density and trabecular strength, and the proliferation, self-renewal, osteogenic differentiation and expression of OCN mRNA of BMMSCs were significantly lower than those of 2-month-old mice. Conclusion: BMMSCs may play an important role in bone senescence.
【作者單位】： 第四軍醫(yī)大學口腔醫(yī)學院;遵義醫(yī)學院;
【基金】：國家重點基礎(chǔ)研究發(fā)展計劃項目(973項目)(2011CB964700)
【分類號】：R363

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