【摘要】：目的:探討卵母細(xì)胞漿內(nèi)單精子注射(intracytoplasmic sperm injection,ICSI)周期中人卵母細(xì)胞形態(tài)學(xué)測(cè)量的指標(biāo)與受精和胚胎發(fā)育潛能之間的關(guān)系。方法:利用OCTAX Eye-ware software系統(tǒng)在對(duì)卵母細(xì)胞行ICSI前,逐一采集卵母細(xì)胞圖像,測(cè)量透明帶厚度、卵母細(xì)胞大小、極體位置的卵間隙大小和極體形態(tài)(完整或破碎),將ICSI后的卵母細(xì)胞和受精后的胚胎進(jìn)行單孔培養(yǎng),記錄每個(gè)卵母細(xì)胞的受精情況、胚胎發(fā)育情況。對(duì)卵母細(xì)胞的形態(tài)數(shù)據(jù)與受精情況、胚胎發(fā)育情況進(jìn)行逐步多元因素Logistic回歸分析。結(jié)果:共測(cè)量卵母細(xì)胞436枚,受精率84.9%(370/436),胚胎可用率60.8%(225/370),卵母細(xì)胞形態(tài)學(xué)指標(biāo)中,透明帶厚度、極體位置的卵間隙大小和卵母細(xì)胞受精與否有關(guān),受精卵較未受精卵有較厚的透明帶[(18.0±2.3)μm vs.(16.9±2.7)μm]和較大的卵間隙[(14.4±3.3)μm vs.(13.2±3.9)μm];而卵母細(xì)胞的大小和極體形態(tài)均與取卵后72 h的胚胎發(fā)育質(zhì)量有關(guān),發(fā)育為可用胚胎的卵母細(xì)胞較發(fā)育為廢用胚胎者有較大的直徑[(116.6±3.7)μm vs.(114.7±3.6)μm]和較高的極體完整率(86.2%vs.66.7%)。結(jié)論:人卵母細(xì)胞形態(tài)學(xué)測(cè)量的指標(biāo)可預(yù)示卵子受精和胚胎發(fā)育的潛能。
[Abstract]:Objective: To study the relationship between the index of human oocyte morphology and the potential of fertilization and embryo development in the period of intracytoplasmic sperm injection (ICSI). Methods: Oocyte images were collected one by one, and the thickness of the zona pellucida, the size of the oocytes, the size of the oocytes and the morphology of the oocytes (intact or broken) were measured by using the OCTAX Eye-ware software system. And carrying out single-hole culture on the oocytes after ICSI and the embryos after fertilization, and recording the fertilization condition and the embryo development condition of each oocyte. The morphological data of the oocytes and the conditions of fertilization and the development of the embryos were analyzed by logistic regression. Results:436 of the oocytes were measured, 84.9% (370/436), 60.8% (225/370) of the embryo availability, the thickness of the zona pellucida, the size of the zona pellucida and the fertilization of the oocytes. The fertilized egg has a thicker zona pellucida[(18.0-2.3). mu.m vs. (16.9-2.7). mu.m] and a larger egg gap[(14.4-3.3). mu.m] and a larger egg gap[(14.4-3.3). mu.m]; and the size and the morphology of the oocyte are all related to the embryo development quality of 72 hours after the oocyte retrieval, Oocytes developed to available embryos were more developed to have a larger diameter[(116.6-3.7). mu.m vs. (114.7[3.6). mu.m] and higher in the complete rate of the embryos (86.2% vs. 66.7%). Conclusion: The index of the morphology of the human oocytes may indicate the potential of the fertilization and the development of the embryo.
【作者單位】： 上海交通大學(xué)醫(yī)學(xué)院附屬瑞金醫(yī)院生殖醫(yī)學(xué)中心;
【基金】：上海市自然科學(xué)基金(09ZR1419000) 上海市科學(xué)技術(shù)委員會(huì)基礎(chǔ)研究重點(diǎn)項(xiàng)目(10JC1410800)資助~~
【分類號(hào)】：R321-33

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