發(fā)布時(shí)間：2019-03-01 14:20

【摘要】：目的觀察Tsp10多肽免疫血清在體外對(duì)旋毛蟲侵入腸上皮細(xì)胞及幼蟲發(fā)育的影響。方法將不同免疫血清與含有旋毛蟲腸道感染性幼蟲的半固體培養(yǎng)基混合后,接種至正常小鼠腸上皮細(xì)胞(intestinal epithelial cells,IEC)單層上,37℃5%CO2培養(yǎng)2h、36h時(shí)分別觀察幼蟲侵入率、細(xì)胞單層受損面積及發(fā)育至Ⅱ~Ⅳ期幼蟲的百分比;免疫熒光試驗(yàn)檢測(cè)受損腸上皮細(xì)胞中的旋毛蟲抗原。結(jié)果幼蟲在半固體培養(yǎng)基培養(yǎng)2h時(shí),Tsp10多肽免疫血清組的幼蟲侵入率與細(xì)胞單層受損面積(46.3%,24.3mm2)均明顯低于T7噬菌體免疫血清組(94.1%,65.3mm2)及正常小鼠血清組(92.5%,65.7mm2)(P0.01);培養(yǎng)36h時(shí),Tsp10多肽免疫血清組Ⅱ~Ⅳ期幼蟲的百分比(16%)亦明顯低于T7噬菌體免疫血清組(37%)及正常小鼠血清組(38%)(P0.01)。Tsp10多肽及ES抗原免疫血清的免疫熒光試驗(yàn)結(jié)果顯示,受損的IEC呈亮綠色熒光,而應(yīng)用T7噬菌體免疫血清及正常小鼠血清時(shí)則為陰性。結(jié)論 Tsp10多肽免疫血清可阻止部分旋毛蟲幼蟲對(duì)腸上皮細(xì)胞的侵入與發(fā)育,Tsp10可能是旋毛蟲的主要侵入蛋白之一。
[Abstract]:Objective to observe the effect of Tsp10 peptide immune serum on the invasion of intestinal epithelial cells and larval development of Trichinella spiralis in vitro. Methods after mixing different immune serum with semisolid medium containing intestinal infective larvae of Trichinella spiralis, they were inoculated into (intestinal epithelial cells,IEC monolayer of normal mouse intestinal epithelial cells, cultured at 37 鈩，

本文編號(hào)：2432503