【摘要】：目的構(gòu)建輸尿管損傷動物模型,篩選輸尿管損傷后纖維化進程中敏感的組織標志物,為進一步研究輸尿管損傷后狹窄繼發(fā)上尿路梗阻提供條件。方法采用雙極電凝法構(gòu)建輸尿管損傷成年雄性新西蘭兔動物模型。損傷后2周獲取患側(cè)輸尿管組織及對側(cè)正常輸尿管組織,觀察狹窄段的大體形態(tài),并行蘇木精-伊紅(H-E)染色觀察狹窄段纖維化程度。應用實時定量反轉(zhuǎn)錄-聚合酶鏈反應(RT-PCR)技術(shù),檢測內(nèi)皮生長因子(EGF)、轉(zhuǎn)化生長因子(TGF)-β、角化生長因子(KGF)、Ⅰ型膠原(ColⅠ)a1、ColⅠa2、Ⅲ型膠原(ColⅢ)和纖維連接蛋白(FN)在受累輸尿管中的表達水平,并與對側(cè)正常輸尿管組織對比,尋找較敏感的纖維化指標。結(jié)果動物模型構(gòu)建成功。動物模型構(gòu)建過程中,狹窄側(cè)輸尿管組織中EGF、TGF-β、FN、ColⅠa1及ColⅠa2水平均顯著高于正常側(cè)輸尿管組織(P值分別0.05、0.01)。結(jié)論雙極電凝法構(gòu)建輸尿管損傷后狹窄新西蘭兔動物模型可行。眾多細胞因子中,EGF、TGF-β、ColⅠa1、ColⅠa2及FN在輸尿管瘢痕形成中起重要作用,可能作為未來干預輸尿管損傷后纖維化的藥物靶點。
[Abstract]:Objective to establish an animal model of ureteral injury and screen sensitive tissue markers in the process of fibrosis after ureteral injury so as to provide conditions for further study of upper urinary tract obstruction secondary to stricture after ureteral injury. Methods the adult male New Zealand rabbit model of ureteral injury was established by bipolar electrocoagulation. The pathological and contralateral ureteral tissues were obtained 2 weeks after injury. The gross morphology of the stenosis was observed and the degree of fibrosis was observed by hematoxylin-eosin (H-E) staining. Real time quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to detect endothelial growth factor (EGF), transforming growth factor (TGF) 尾 and keratinocyte growth factor (KGF), type 鈪，

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