發(fā)布時(shí)間：2018-11-19 19:07

【摘要】：目的:研究人腦與大鼠新皮層FS神經(jīng)元的電生理學(xué)特征及異同點(diǎn)。方法:應(yīng)用手術(shù)切除非病理皮層組織制備和培養(yǎng)人腦腦片,利用全細(xì)胞膜片鉗記錄FS神經(jīng)元的內(nèi)在電生理特征,比較人腦與大鼠新皮層FS神經(jīng)元電生理學(xué)差異。結(jié)果:(1)根據(jù)神經(jīng)元在閾強(qiáng)度水平最初發(fā)放的動(dòng)作電位放電模式,可將人腦FS神經(jīng)元分為三種類型:典型性FS神經(jīng)元(c-FS),爆發(fā)性FS神經(jīng)元(b-FS)及延遲性FS神經(jīng)元(d-FS)。這三種亞型類似于大鼠大腦皮層FS神經(jīng)元的電生理學(xué)分類亞型。(2)人腦FS神經(jīng)元的動(dòng)作電位閾值、后超極化幅度及輸入電流/輸出頻率斜率(I/O slope)均較大鼠增高,而靜息電位、動(dòng)作電位半寬及閾強(qiáng)度均較大鼠降低。人腦皮層神經(jīng)元?jiǎng)幼麟娢话雽捄蛣?dòng)作電位后超極化幅度(人:-24.61±2.45)較大鼠神經(jīng)元的差異具有顯著性意義(P0.05)。(3)當(dāng)輸入相同電流時(shí)人腦皮層神經(jīng)元?jiǎng)幼麟娢坏妮敵鲱l率大于大鼠神經(jīng)元,當(dāng)輸入電流為75 p A時(shí)具有顯著性差異(P0.05)。結(jié)論:(1)人腦FS神經(jīng)元的電生理學(xué)分類與大鼠相似;(2)人腦FS神經(jīng)元與大鼠存在電生理學(xué)差異;(3)人腦FS神經(jīng)元在輸入電流接近75 p A時(shí)動(dòng)作電位頻率與大鼠存在差異。
[Abstract]:Aim: to study the electrophysiological characteristics and similarities and differences of FS neurons in human brain and rat neocortex. Methods: brain slices were prepared and cultured by surgical excision of non-pathological cortical tissue. The intrinsic electrophysiological characteristics of FS neurons were recorded by whole-cell patch clamp. The electrophysiological differences of FS neurons between human brain and rat neocortex were compared. Results: (1) according to the pattern of action potential discharge initially released by neurons at threshold intensity level, human FS neurons can be classified into three types: typical FS neurons (c-FS). Explosive FS neurons (b-FS) and delayed FS neurons (d-FS). These three subtypes are similar to the electrophysiological subtypes of FS neurons in rat cerebral cortex. (2) the threshold of action potential of human brain FS neurons, the amplitude of posthyperpolarization and the I / O slope increase. The resting potential, half width of action potential and threshold intensity decreased significantly. There were significant differences in the half width of action potential and the amplitude of hyperpolarization after action potential (human: -24.61 鹵2.45) in cerebral cortical neurons (P0.05). (3). The output frequency of action potential of laminar neurons was higher than that of rat neurons. There was significant difference when the input current was 75 Pa (P0.05). Conclusion: (1) the electrophysiological classification of FS neurons in human brain is similar to that in rats, (2) there are differences in electrophysiology between human brain FS neurons and rats. (3) the frequency of action potential of human brain FS neurons was different from that of rats when the input current was close to 75 Pa.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R338

【參考文獻(xiàn)】

中國(guó)碩士學(xué)位論文全文數(shù)據(jù)庫(kù) 前1條

1 王玉晶;神經(jīng)科學(xué)研究中人類腦片培養(yǎng)技術(shù)的建立和評(píng)價(jià)[D];吉林大學(xué);2015年

，

本文編號(hào)：2343174