發(fā)布時間：2018-11-19 19:07

【摘要】：目的:研究人腦與大鼠新皮層FS神經元的電生理學特征及異同點。方法:應用手術切除非病理皮層組織制備和培養(yǎng)人腦腦片,利用全細胞膜片鉗記錄FS神經元的內在電生理特征,比較人腦與大鼠新皮層FS神經元電生理學差異。結果:(1)根據神經元在閾強度水平最初發(fā)放的動作電位放電模式,可將人腦FS神經元分為三種類型:典型性FS神經元(c-FS),爆發(fā)性FS神經元(b-FS)及延遲性FS神經元(d-FS)。這三種亞型類似于大鼠大腦皮層FS神經元的電生理學分類亞型。(2)人腦FS神經元的動作電位閾值、后超極化幅度及輸入電流/輸出頻率斜率(I/O slope)均較大鼠增高,而靜息電位、動作電位半寬及閾強度均較大鼠降低。人腦皮層神經元動作電位半寬和動作電位后超極化幅度(人:-24.61±2.45)較大鼠神經元的差異具有顯著性意義(P0.05)。(3)當輸入相同電流時人腦皮層神經元動作電位的輸出頻率大于大鼠神經元,當輸入電流為75 p A時具有顯著性差異(P0.05)。結論:(1)人腦FS神經元的電生理學分類與大鼠相似;(2)人腦FS神經元與大鼠存在電生理學差異;(3)人腦FS神經元在輸入電流接近75 p A時動作電位頻率與大鼠存在差異。
[Abstract]:Aim: to study the electrophysiological characteristics and similarities and differences of FS neurons in human brain and rat neocortex. Methods: brain slices were prepared and cultured by surgical excision of non-pathological cortical tissue. The intrinsic electrophysiological characteristics of FS neurons were recorded by whole-cell patch clamp. The electrophysiological differences of FS neurons between human brain and rat neocortex were compared. Results: (1) according to the pattern of action potential discharge initially released by neurons at threshold intensity level, human FS neurons can be classified into three types: typical FS neurons (c-FS). Explosive FS neurons (b-FS) and delayed FS neurons (d-FS). These three subtypes are similar to the electrophysiological subtypes of FS neurons in rat cerebral cortex. (2) the threshold of action potential of human brain FS neurons, the amplitude of posthyperpolarization and the I / O slope increase. The resting potential, half width of action potential and threshold intensity decreased significantly. There were significant differences in the half width of action potential and the amplitude of hyperpolarization after action potential (human: -24.61 鹵2.45) in cerebral cortical neurons (P0.05). (3). The output frequency of action potential of laminar neurons was higher than that of rat neurons. There was significant difference when the input current was 75 Pa (P0.05). Conclusion: (1) the electrophysiological classification of FS neurons in human brain is similar to that in rats, (2) there are differences in electrophysiology between human brain FS neurons and rats. (3) the frequency of action potential of human brain FS neurons was different from that of rats when the input current was close to 75 Pa.
【學位授予單位】：吉林大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R338

【參考文獻】

中國碩士學位論文全文數據庫 前1條

1 王玉晶;神經科學研究中人類腦片培養(yǎng)技術的建立和評價[D];吉林大學;2015年

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