【摘要】：目的觀察武漢地區(qū)手足口病(HFMD)患兒腸道病毒71型(EV71)的基因型及其重組特點(diǎn)。方法收集2011年4月至2012年3月武漢地區(qū)1 844例HFMD患兒EV71陽性的咽拭子標(biāo)本并進(jìn)行病毒分離鑒定,對124株EV71病毒分離株的508 bp VP1基因序列進(jìn)行測序,選擇2株來自重癥和1株來自輕癥患兒的EV71毒株,構(gòu)建VP1序列系統(tǒng)進(jìn)化樹并分析其全基因組序列的重組特點(diǎn)。結(jié)果 VP1序列系統(tǒng)進(jìn)化分析顯示,124株EV71型均屬于C4基因型,其中56株屬于C4a亞群,68株屬于C4b亞群,Bootscan和5'UTR、P1、P2、P3區(qū)的進(jìn)化樹證實(shí)3株EV71分離株均為親本株C4a型毒株guangdong/GD/CHN/2009和C4b型毒株SHZH98/GD/CHN/1998在P2區(qū)2A-2B基因交界處發(fā)生型內(nèi)重組所產(chǎn)生。結(jié)論 2011年4月至2012年3月武漢市及周邊地區(qū)的EV71分離株均為C4基因型,與1998年以來中國大陸的優(yōu)勢株流行一致,且C4基因型存在型內(nèi)重組現(xiàn)象。
[Abstract]:Objective to observe the genotype and recombinant characteristics of enterovirus 71 (EV71) in children with hand-foot-mouth disease (HFMD) in Wuhan. Methods from April 2011 to March 2012, EV71 positive throat swabs of 1 844 children with HFMD were collected and identified. The 508 bp VP1 gene sequences of 124 EV71 virus isolates were sequenced. Two EV71 strains from severe disease and one from mild disease were selected to construct the phylogenetic tree of VP1 sequence and analyze the recombination characteristics of the whole genome sequence. Results the phylogenetic analysis of VP1 sequence showed that the EV71 genotype of 124strain belonged to C4 genotype. Among them, 56 strains belong to C4a subgroup, 68 strains belong to C4b subgroup Bootscan and 5UTRP1P2P2P3 region. It is confirmed that the three isolates of EV71 were all generated by recombination of parental strain C4a strain guangdong/GD/CHN/2009 and C4b strain SHZH98/GD/CHN/1998 at the junction of 2A-2B gene in P2 region. Conclusion from April 2011 to March 2012, EV71 isolates from Wuhan and its surrounding areas were all C4 genotypes, which were consistent with the prevalence of dominant strains in mainland China since 1998.
【作者單位】： 武漢市醫(yī)療救治中心檢驗(yàn)科;武漢大學(xué)生命科學(xué)院;
【分類號】：R373.2

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 ;Characterization of Hand,Foot,and Mouth Disease in China between 2008 and 2009[J];Biomedical and Environmental Sciences;2011年03期

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 李華;邵聰文;潘s，

本文編號：2211764