本文選題：動脈粥樣硬化 + 電針　； 參考：《遼寧中醫(yī)藥大學》2017年碩士論文

【摘要】：目的:觀察電針內(nèi)關(guān)、關(guān)元、足三里穴對動脈粥樣硬化模型兔的干預作用,通過檢測各組實驗兔血清中MIF、IL-10表達水平,探討電針內(nèi)關(guān)、關(guān)元、足三里穴抑制動脈粥樣硬化炎癥反應的作用機制。材料與方法:普通級雄性新西蘭兔26只,體重2.0-2.5kg,3月齡。采用高脂飼料喂養(yǎng)聯(lián)合頸總動脈球囊損傷術(shù)法建立動脈粥樣硬化模型。將26只實驗兔隨機分成兩組:空白對照組(7只)、造模組(19只)�？瞻讓φ战M,給予普通飼料進行喂養(yǎng);造模組,予高脂飼料喂養(yǎng),并于造模第4周施以頸總動脈球囊損傷術(shù),術(shù)后繼續(xù)高脂飼料喂養(yǎng)4周。進行造模干預8周后,從兩組各隨機選取1只實驗兔處死,取其頸總動脈采用HE染色法觀察動脈粥樣斑塊形成情況,以確認模型復制成功與否。確認造模成功后,空白對照組剩余實驗兔(6只)繼續(xù)如前喂養(yǎng);采用隨機數(shù)字表法將造模組剩余實驗兔(18只)均分為三組,分別為:AS模型組(6只)、電針干預組(6只)、陽性藥物對照組(6只),均予高脂飼料喂養(yǎng),并開始治療,6日為1療程,各療程間休息1日,連續(xù)進行4個療程。治療方法:電針干預組予針刺內(nèi)關(guān)穴、關(guān)元穴、足三里穴治療,進針后,于同側(cè)“內(nèi)關(guān)穴、足三里穴”,關(guān)元穴和兔尾非經(jīng)非穴的針柄處接通電針治療儀,予疏密波,強度以實驗兔肢體輕顫、能夠耐受不煩躁為度,電流1mA,留針20 min,1次/d;陽性藥物對照組予阿托伐他汀鈣片1mg/kg/d制成懸濁液灌胃治療。第4療程結(jié)束后,于實驗兔耳緣靜脈采血待測,再行耳緣靜脈空氣栓塞法處死后,予頸動脈取材,采用HE染色法制備病理切片,觀察頸動脈組織的病理形態(tài)學變化,用酶聯(lián)免疫吸附法(ELISA,Enzyme Linked Immunosorbent Assay)測定兔血清中巨噬細胞移動抑制因子(MIF)和白介素-10(IL-10)的含量,探討電針刺激內(nèi)關(guān)、關(guān)元、足三里穴對動脈粥樣硬化的治療作用機制。結(jié)果:1.光鏡下觀察:空白對照組兔頸總動脈內(nèi)膜完整連續(xù),無炎癥細胞浸潤及泡沫細胞形成;AS模型組兔頸總動脈內(nèi)膜明顯增厚,內(nèi)皮細胞不連續(xù),單核細胞浸潤及聚集增多,大量泡沫細胞聚集成斑塊;電針干預組及陽性藥物對照組兔頸總動脈內(nèi)膜增厚,內(nèi)皮細胞不連續(xù),可見單核細胞浸潤,有泡沫細胞形成,較AS模型組病變程度明顯減輕。2.與AS模型組相比較,電針及陽性藥物治療均可下調(diào)AS兔血清中MIF的含量。AS模型組與各組結(jié)果比較有顯著差異(P0.05);比較電針干預組與陽性藥物對照組血清中MIF水平,無明顯差異(P0.05)。3.與AS模型組相比較,電針及陽性藥物干預均可上調(diào)AS兔血清中IL-10的含量。AS模型組與各組比較有顯著差異(P0.05);比較電針干預組與陽性藥物對照組血清中IL-10水平,無明顯差異(P0.05)。結(jié)論:1.電針內(nèi)關(guān)、關(guān)元、足三里穴,可通絡(luò)活血化痰,從而抑制動脈粥樣斑塊形成。2.電針內(nèi)關(guān)、關(guān)元、足三里穴,抑制兔血清中促炎因子MIF的表達,促進血清中抗炎因子IL-10的表達,可能通過通絡(luò)活血化痰抑制炎癥反應,進而控制動脈粥樣硬化的發(fā)展。
[Abstract]:Objective: To observe the effect of electroacupuncture Neiguan, Guan yuan and Zusanli on the atherosclerotic rabbit model, and to explore the mechanism of the effect of electroacupuncture Neiguan, Guan yuan and Zusanli on the inhibition of atherosclerotic inflammation by detecting the MIF and IL-10 expression levels in the serum of all experimental rabbits. Materials and methods: 26 normal male New Zealand rabbits, weighing 2.0-2 .5kg, 3 month old. A model of atherosclerosis was established by feeding high fat diet combined with balloon injury of common carotid artery. 26 rabbits were randomly divided into two groups: blank control group (7) and model group (19). The blank control group was fed with ordinary feed, the model group, high fat feed were given, and the common carotid artery was given for fourth weeks. After the operation, the high fat diet was continued for 4 weeks. After 8 weeks of model intervention, 1 rabbits were randomly selected from two groups, and the carotid artery plaque formation was observed by HE staining, to confirm the success of the Model replica. After the model was successful, the remaining rabbits in the blank control group (6) continued to be fed as pre feeding. The experiment rabbits (18 rats) were divided into three groups by random digital table method: AS model group (6), electroacupuncture intervention group (6), positive drug control group (6), all were fed with high fat diet, and the treatment was given, the 6 day was 1 courses, and 1 days in each course. The treatment method: acupuncture intervention group was given acupuncture. Guan acupoint, Guan yuan acupoint, Zusanli treatment, after entering the needle, at the same side "Neiguan point, Zusanli point", Guan yuan point and the tail of the rabbit at the needle handle of non acupoints through the acupuncture treatment instrument, the intensity of the density wave, the experimental rabbit limb tremble, can tolerate no irritability, current 1mA, 20 min, 1 /d; positive drug control group to Atorvastatin Calcium Tablets 1mg/ After the fourth course of the fourth course of treatment, after the end of the fourth course of treatment, the blood collection of the rabbit ear vein was measured. After the ear vein air embolism was executed, the carotid artery was selected and the pathological section was prepared by HE staining method. The pathological changes of the carotid tissue were observed, and the enzyme linked immunosorbent assay (ELISA, Enzyme Linked Immunosorbent Assay) was used. The contents of macrophage migration inhibitory factor (MIF) and interleukin -10 (IL-10) in rabbit serum were measured, and the therapeutic mechanism of electroacupuncture stimulation of Neiguan, Guan yuan and Zusanli on atherosclerosis was discussed. Results: 1. light microscopy showed that there was no inflammatory cell infiltration and foam cell formation in the blank control group. No inflammatory cell infiltration and foam cell formation; AS model. The intima of the common carotid artery in the rabbits was thickened, the endothelial cells were discontinuous, the infiltration and aggregation of monocytes increased, and a large number of foam cells were aggregated. The intima of the common carotid artery in the electroacupuncture intervention group and the positive drug control group were thickened, the endothelial cells were discontinuous, the monocyte infiltration and the formation of foamy cells were visible, and the degree of pathological changes in the AS model group was significantly reduced. Comparison between light.2. and AS model group, electroacupuncture and positive drug treatment could reduce the content of MIF in AS rabbit serum.AS model group compared with the results of each group was significantly different (P0.05); compared with the positive drug control group and the positive drug control group, there was no significant difference in serum MIF level (P0.05).3. and AS model group, electroacupuncture and positive drug intervention could be compared. There was significant difference between the.AS model group and the IL-10 in the serum of AS rabbit serum (P0.05), and there was no significant difference in the serum IL-10 level between the electroacupuncture intervention group and the positive drug control group (P0.05). Conclusion: 1. electroacupuncture Neiguan, Guan yuan, Zusanli point can collaterals and activate blood phlegm, thus inhibiting the formation of atherosclerotic plaque of the.2. electroacupuncture Neiguan, Guan yuan, foot. The three li point inhibits the expression of pro-inflammatory factor MIF in rabbit serum and promotes the expression of anti inflammatory factors IL-10 in serum. It may inhibit the inflammatory reaction through activating blood and activating blood and eliminating phlegm, and then controlling the development of atherosclerosis.
【學位授予單位】：遼寧中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R245;R-332

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