本文選題：樹突狀細(xì)胞 + 白念珠菌��； 參考：《現(xiàn)代免疫學(xué)》2013年03期

【摘要】：為分析白念珠菌活化的樹突狀細(xì)胞microRNA表達(dá)譜的變化,進(jìn)而為研究microRNA在白念珠菌引發(fā)的固有免疫應(yīng)答過程中的調(diào)節(jié)作用提供參考。我們采用microRNA芯片法分析了白念珠菌刺激前后人單核源性樹突狀細(xì)胞內(nèi)microRNA表達(dá)譜的變化,采用MicroCosm v5、TargetScan 5.1和MICRORNA.ORG預(yù)測差異表達(dá)的microRNA的靶點(diǎn),并對這些靶點(diǎn)進(jìn)行GO和pahway分析。結(jié)果表明白念珠菌可以上調(diào)人單核源性樹突狀細(xì)胞內(nèi)62個(gè)microRNA的表達(dá),下調(diào)4個(gè)microRNA的表達(dá)。這些差異表達(dá)的microRNA的理論靶點(diǎn)廣泛地參與了多個(gè)生物學(xué)過程以及多條信號通路。
[Abstract]:It is useful to analyze the expression profile of microRNA in dendritic cells activated by Candida albicans and to study the regulatory role of microRNA in the innate immune response induced by Candida albicans. The microRNA expression profiles of monocyte derived dendritic cells stimulated by Candida albicans were analyzed by microRNA-chip method. The targets of differentially expressed microRNAs were predicted by MicroCosm v5TargetScan 5.1 and MICRORNA.ORG, and these targets were analyzed by go and pahway. The results showed that Candida albicans could up-regulate the expression of 62 microRNAs and down-regulate the expression of 4 microRNAs in human mononuclear dendritic cells. The theoretical targets of these differentially expressed microRNAs are widely involved in multiple biological processes and multiple signaling pathways.
【作者單位】： 濟(jì)南軍區(qū)總醫(yī)院實(shí)驗(yàn)診斷科;第二軍醫(yī)大學(xué)長征醫(yī)院實(shí)驗(yàn)診斷科;第二軍醫(yī)大學(xué)長海醫(yī)院實(shí)驗(yàn)診斷科;第二軍醫(yī)大學(xué)長征醫(yī)院;
【基金】：國家自然科學(xué)金(30972730;81273282) 上海市科委基金(11JC1410902) 長海醫(yī)院基金(CH125530300)
【分類號】：R392

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 郭寧如,沈永年,呂桂霞,吳紹熙;不同免疫狀態(tài)下的白念珠菌對小鼠腸粘膜的粘附及影響[J];中華皮膚科雜志;1994年02期

2 于益芝;王建莉;曹雪濤;;樹突狀細(xì)胞分化發(fā)育的研究進(jìn)展[J];國外醫(yī)學(xué)(免疫學(xué)分冊);1998年06期

3 姚敬業(yè)!230061,青木茂治,周家華!230061,久和彰江,楊遠(yuǎn)麗!230061,仲村健二郎,鄭芹!230061;中國合肥與日本新，

本文編號：2041081