本文選題：骨髓間充質干細胞 + 腫瘤壞死因子-α�。� 參考：《中國實驗血液學雜志》2013年06期

【摘要】：本研究目的在于探討TNF-α對人骨髓間充質干細胞(bone marrow mesenchymal stem cells,BMMSC)血管細胞黏附分子-1(vascular cell adhesion molecule 1,VCAM-1)表達的影響及與ERK信號通路的關系。應用密度梯度離心法結合貼壁培養(yǎng)法分離人BMMSC,并對其進行表面抗原表達及誘導分化鑒定。用不同濃度的TNF-α刺激BMMSC,流式細胞術檢測其表面VCAM-1表達及ERK信號通路抑制劑U0126對BMMSC VCAM-1表達的影響,用Western blot檢測ERK通路活性的變化。結果表明,分離培養(yǎng)的BMMSC表達CD29、CD69、CD44、CD105,不表達CD34、CD45;BMMSC可誘導分化為成骨細胞及脂肪細胞;BMMSC膜上的VCAM-1表達隨著TNF-α濃度的增加而增加;經(jīng)U0126預處理后TNF-α刺激的BMMSC膜上的VCAM-1表達減少;TNF-α增加BMMSC ERK信號通路的活化程度,U0126抑制TNF-α對ERK活性的作用。結論:TNF-α可能通過ERK信號通路刺激MSC表達VCAM-1。
[Abstract]:The aim of this study was to investigate the effect of TNF- 偽 on the expression of vascular adhesion molecule-1 (- 1) and vascular cell adhesion molecule 1 (VCAM-1) in human bone marrow mesenchymal stem cells (BMSCs) and the relationship between TNF- 偽 and ERK signaling pathway. Human BMMSCS were isolated by density gradient centrifugation combined with adherent culture, and their surface antigen expression and differentiation were identified. BMMSCs were stimulated with different concentrations of TNF- 偽. The expression of VCAM-1 on the surface and the effect of U0126, a signal pathway inhibitor of ERK, on the expression of BMMSC VCAM-1 were detected by flow cytometry. The activity of ERK pathway was detected by Western blot. The results showed that BMMSC expressed CD29, CD69, CD44, CD105, and no CD34, CD45, BMMSC could induce differentiation into osteoblasts and adipocytes, and the VCAM-1 expression on BMMSC membrane increased with the increase of TNF- 偽 concentration. After pretreatment with U0126, the expression of VCAM-1 on BMMSC membrane stimulated by TNF- 偽 decreased and TNF- 偽 increased the activation of BMMSC ERK signaling pathway. U0126 inhibited the effect of TNF- 偽 on the activity of ERK. ConclusionTNF- 偽 may stimulate MSC expression of VCAM-1through ERK signaling pathway.
【作者單位】： 南方醫(yī)科大學;廣州軍區(qū)廣州總醫(yī)院血液科;
【基金】：全軍醫(yī)學科學技術研究"十二五"重點課題(編號BWS11J071) 國家自然基金青年項目(編號81101471/H1602) 廣州市科技計劃項目(編號2013J2200024)
【分類號】：R329.28

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本文編號：1946153