發(fā)布時間：2018-04-24 04:08

  本文選題：云南春燥 + 氣道��； 參考：《云南中醫(yī)學(xué)院》2017年碩士論文

【摘要】：目的:本課題依據(jù)中醫(yī)病因?qū)W說模擬云南春燥環(huán)境,并結(jié)合病理學(xué)、現(xiàn)代免疫學(xué)等相關(guān)知識,通過觀察云南春燥環(huán)境下小鼠氣管和肺組織病理形態(tài)的變化以及氣道分泌型免疫球蛋白A(sIgA)、免疫球蛋白G(IgG)、肺表面活性物質(zhì)相關(guān)蛋白A(SP-A)、肺表面活性物質(zhì)相關(guān)蛋D(SP-D)含量的變化,以探究云南春燥環(huán)境對小鼠肺系津液耗傷,以及肺宣肅功能、衛(wèi)外功能的影響。初步從免疫分子水平角度闡釋云南春燥的內(nèi)在本質(zhì),為下一步開展云南春燥動物模型研究提供客觀依據(jù)。方法:借鑒當(dāng)前中醫(yī)燥證實驗研究方法,依據(jù)中醫(yī)病因?qū)W說,結(jié)合云南春燥環(huán)境的特點,利用人工氣候箱設(shè)置“溫度-相對濕度-風(fēng)”空間量化指標(biāo),綜合刺激模擬云南春燥氣候環(huán)境所致外因;再喂以辛香干燥食物,以模擬云南地域飲食特點,并作用于昆明種小鼠,同時設(shè)立正常對照組、食物組、氣候組、氣候食物組。每天觀察記錄各組小鼠飲食量、飲水量、大便性狀、被毛等變化,并于第7天、第14天分別進(jìn)行以下實驗:采用HE染色法光鏡下觀察各組實驗小鼠氣管和肺組織病理形態(tài)的變化;采用酶聯(lián)免疫吸附法,檢測各組實驗小鼠氣道sIgA、IgG、SP-A、SP-D含量的變化。結(jié)果:1.小鼠一般體征的觀察正常對照組,精神充沛,被毛光澤,大便質(zhì)軟,無打斗現(xiàn)象;食物組,精神尚可,被毛較為光澤,與正常對照組相比體重略有減輕,飲食量、飲水量增加,小便量減少,大便質(zhì)干,未見打斗現(xiàn)象;氣候組,情緒逐漸煩躁,多見打斗撕咬狀態(tài),被毛發(fā)黃少澤,與正常對照組相比體重明顯減輕,飲食量、飲水量增加,小便量減少,大便尚可;氣候食物組,情緒略顯煩躁,偶見打斗撕咬狀態(tài),被毛發(fā)黃疏松,與正常對照組相比體重減輕,飲食量減少,飲水量增加,小便量減少,大便質(zhì)地較干。2.小鼠氣管及肺組織病理形態(tài)的觀察正常對照組,在第7天、第14天氣管管腔形態(tài)規(guī)則,肺泡壁完整,未見明顯黏膜損傷及炎性細(xì)胞浸潤;食物組,在第7天,氣管黏膜損傷脫落,肺泡壁毛細(xì)血管充血,有炎性細(xì)胞浸潤。第14天,氣管黏膜層褶皺損傷脫落,并見少量肺大泡及少許炎性細(xì)胞;氣候組,在第7天、第14天氣管黏膜增生伴脫落,肺泡壁毛細(xì)血管充血,并見黏膜層及黏膜下層有少許炎性細(xì)胞,且第14天較第7天為重。氣候食物組,在第7天,氣管黏膜層損傷脫落,肺泡壁毛細(xì)血管充血,黏膜下層輕微炎性細(xì)胞浸潤。在第14天,氣管黏膜層損傷脫落,可見炎性細(xì)胞浸潤及肺大泡。3.氣道sIgA、IgG含量檢測sIgA含量:與正常對照組相比,在第7天,食物組sIgA含量降低,具有明顯差異性(P0.05),氣候組、氣候食物組sIgA含量降低,差異無統(tǒng)計學(xué)意義(P0.05);在第14天,食物組、氣候組、氣候食物組sIgA含量降低,具有明顯差異性(P0.05)。IgG含量:與正常對照組比較,在第7天,食物組、氣候組、氣候食物組IgG含量升高,具有明顯差異性(P0.05);在第14天,食物組IgG含量升高,差異無統(tǒng)計學(xué)意義(P0.05);氣候組、氣候食物組IgG含量升高,具有明顯差異性(P0.05)。4.氣道SP-A、SP-D含量檢測SP-A含量:與正常對照組比較,在第7天,食物組、氣候組、氣候食物組SP-A含量降低,差異無統(tǒng)計學(xué)意義(P0.05);在第14天,食物組、氣候組SP-A含量降低,差異無統(tǒng)計學(xué)意義(P0.05),氣候食物組SP-A含量降低,具有明顯差異性(P0.05)。SP-D含量:與正常對照組比較,在第7天,食物組、氣候組、氣候食物組SP-D含量升高,差異無統(tǒng)計學(xué)意義(P0.05);在第14天,食物組SP-D含量升高,差異無統(tǒng)計學(xué)意義(P0.05),氣候組、氣候食物組SP-D含量升高,具有明顯差異性(P0.05)。結(jié)論:1.云南春燥環(huán)境可使小鼠的生物表征呈現(xiàn)一系列的干燥變化,且隨著時間的延長,這種改變更加明顯。2.云南春燥環(huán)境可影響小鼠氣管及肺組織病理形態(tài)的改變。3.云南春燥環(huán)境可使小鼠氣道內(nèi)sIgA、SP-A含量下降,SP-D、IgG含量上升。4.本研究說明云南春燥環(huán)境,能影響肺宣發(fā)津液及衛(wèi)氣的功能。初步揭示了燥勝則干,燥邪犯肺,肺津不化,衛(wèi)表不固的病機(jī)實質(zhì)。
[Abstract]:Objective: to simulate the environment of Yunnan spring dryness according to the etiology of traditional Chinese medicine, and to observe the pathological changes of the trachea and lung in the spring dryness environment of Yunnan, the changes in the pathological morphology of the trachea and lung tissue in the spring dryness environment of Yunnan, the airway secretory immunoglobulin A (sIgA), the immunoglobulin G (IgG) and the pulmonary surfactant related protein A (SP-A). The changes in the content of D (SP-D) of the pulmonary surfactant related to the eggs of the Yunnan spring dryness environment have been used to explore the influence of the injury of the body fluid in the lung system of the mice, the function of the lung Xuan Su and the outside function. The intrinsic essence of the spring dryness in Yunnan is explained preliminarily from the angle of the immune molecular level, and the objective basis is to provide the objective basis for the further study of the model of Yunnan southern spring dryness. Based on the etiological theory of traditional Chinese medicine, according to the etiological theory of traditional Chinese medicine and the characteristics of the environment of spring dryness in Yunnan, the quantitative index of "temperature relative humidity wind" was set up by artificial climate box, and the external cause was stimulated to simulate the climate environment of spring dryness in Yunnan, and the dry food of Xinxiang was fed to simulate the food characteristics of Yunnan region. In Kunming, a normal control group, a food group, a climate group and a climate food group were set up at the same time. The diet, drinking water, stool traits, and hair were observed every day, and the following experiments were carried out on seventh days and fourteenth days. The pathological changes of the trachea and lung tissue in each group were observed under the HE staining method. The changes in the content of sIgA, IgG, SP-A and SP-D in the airway of all the mice were detected by enzyme linked immunosorbent assay. Results: 1. the general signs of the mice were observed in the normal control group with abundant spirit, Mao Guangze, stool soft, no fighting phenomenon; the food group, the spirit was still good, the hair was more luster, and the weight a little lightened and diet compared with the normal control group. The amount of drinking water increased, the amount of stool decreased, the stool was dry, no fighting phenomenon was found; the climate group, the mood gradually irritated, and the fights and tearing States, the hair yellow less, compared with the normal control group, the weight was obviously reduced, the amount of diet, the amount of drinking water increased, the stool decreased, the stool was still available; the climate food group, the mood slightly irritable, occasionally see the state of fight and tearing. The hair was loosely yellow, compared with the normal control group, the weight loss, the decrease of the diet, the increase of the drinking water, the decrease of the amount of urine, the normal control group of the trachea and lung tissue of the dry.2. mice were observed in the normal control group. In the seventh day, the shape of the tube cavity, the alveolar wall, no obvious mucosal injury and inflammatory cell infiltration were found, and the food group, On the seventh day, the damage of the trachea mucous membrane, the capillary blood capillary of the alveolar wall and the inflammatory cell infiltration. On the fourteenth day, the tracheal mucosa folds and wrinkles were lost, and a small amount of pulmonary bullae and a few inflammatory cells were seen. In the climate group, the mucous membrane and the mucous membrane and the submucosa were less phlogistic on the seventh day, fourteenth weather, mucous membrane and capillaries. On the seventh day, the injury of the trachea mucous membrane was lost, the alveolar capillary was congested and the submucosal layer was slight inflammatory cell infiltration. On the fourteenth day, the injury of the trachea mucous membrane was lost, and the inflammatory cell infiltration and the.3. sIgA of the pulmonary bullae were seen, and the content of IgG was detected in the sIgA content: compared with the normal control group, On the seventh day, the content of sIgA in the food group decreased (P0.05), the sIgA content of the climate group and the climate food group decreased, and the difference was not statistically significant (P0.05). In the fourteenth day, the sIgA content of the food group, the climate group and the climate food group decreased (P0.05).IgG content: compared with the normal control group, the food group, the food group, and the climate group were compared with the normal control group. Climate food group IgG content increased significantly (P0.05); in the fourteenth day, the food group IgG content increased, the difference was not statistically significant (P0.05); climate group, the climate food group IgG content increased, there was a significant difference (P0.05).4. airway SP-A, SP-D content detected SP-A content: compared with the normal control group, in the seventh day, food group, climate group, gas. There was no significant difference in SP-A content in the food group (P0.05). In the fourteenth day, the food group, the climate group SP-A content decreased, the difference was not statistically significant (P0.05), the SP-A content of the climate food group decreased, and there was a significant difference (P0.05).SP-D content: compared with the normal group, the food group, the climate group, the climate food group SP-D content rose on the seventh day. The difference was not statistically significant (P0.05). In the fourteenth day, the content of SP-D in the food group increased, and the difference was not statistically significant (P0.05). The SP-D content in the climate group and the climate food group increased significantly (P0.05). Conclusion: 1. Yunnan spring dryness environment can make a series of dry changes in the biological characterization of mice, and this change along with the time of the change. More obvious.2. Yunnan spring dryness environment can affect the pathological changes in the trachea and lung tissue of mice.3. Yunnan spring dryness environment can reduce the content of sIgA and SP-A in the airway of mice, SP-D, IgG content increases.4.. This study shows that the spring dryness environment in Yunnan can affect the energy of the body fluid and Wei Qi of the lung. The essence of the disease is not fixed.

【學(xué)位授予單位】：云南中醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R-332;R228

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