電針對(duì)動(dòng)脈粥樣硬化模型家兔血清中CD40、CD40L影響的實(shí)驗(yàn)研究
本文選題:動(dòng)脈粥樣硬化 + CD40; 參考:《遼寧中醫(yī)藥大學(xué)》2017年碩士論文
【摘要】:目的:觀察電針內(nèi)關(guān)、關(guān)元、足三里穴對(duì)動(dòng)脈粥樣硬化模型家兔的干預(yù)作用,通過(guò)檢測(cè)各組血清中CD40、CD40L的表達(dá)水平,探討電針內(nèi)關(guān)、關(guān)元、足三里穴抑制動(dòng)脈粥樣硬化模型家兔炎癥反應(yīng)的作用機(jī)制。材料與方法:應(yīng)用26只健康雄性新西蘭雄性家兔(每只2-2.5kg,2-3月齡),適應(yīng)性喂養(yǎng)一周后,隨機(jī)分為正常組和造模組,其中正常組7只,造模組19只。正常組給予普通飼料喂養(yǎng),造模組給予高脂飼料和普通飼料混合喂養(yǎng),高脂喂養(yǎng)4周后對(duì)造模組行頸動(dòng)脈球囊損傷術(shù)。高脂喂養(yǎng)8周后,從正常組和造模組中各隨機(jī)選取1只家兔檢測(cè)造模是否成功,采用HE染色的檢測(cè)方法觀察家兔頸動(dòng)脈粥樣硬化斑塊病理形態(tài)學(xué)變化。將造模組(18只)隨機(jī)分為三組(每組n=6):模型組、電針組、西藥組。電針組選取內(nèi)關(guān)(雙側(cè))、足三里(雙側(cè))、關(guān)元穴進(jìn)行治療,每日1次;西藥組給予阿托伐他汀鈣片懸濁液1mg/kg/d灌胃給藥。6天為1療程,療程間休息1日,共4個(gè)療程。第四個(gè)療程治療結(jié)束后對(duì)所有家兔進(jìn)行處死,耳緣經(jīng)脈采血,分離血清,取頸動(dòng)脈固定于4%多聚甲醛中48小時(shí)后HE染色,分析頸動(dòng)脈的病理學(xué)改變,采用酶聯(lián)免疫吸附測(cè)定法對(duì)血清中CD40、CD40L進(jìn)行分析檢測(cè)。結(jié)果:1.電針內(nèi)關(guān)、關(guān)元、足三里穴對(duì)血清中CD40的影響:與正常組進(jìn)行比較,模型組、電針組、西藥組血清中CD40的表達(dá)增高,差異具有統(tǒng)計(jì)學(xué)意義(P0.05);與模型組比較,電針組和西藥組家兔血清中CD40表達(dá)水平降低,差異有統(tǒng)計(jì)學(xué)意義(P0.05);與西藥組比較,電針組血漿CD40水平無(wú)明顯差異,差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。2.電針內(nèi)關(guān)、關(guān)元、足三里穴對(duì)血清中CD40L的影響:與正常組進(jìn)行比較,模型組、電針組、西藥組血清中CD40L的表達(dá)增高,差異具有統(tǒng)計(jì)學(xué)意義(P0.05);與模型組比較,電針組和西藥組家兔血清中CD40L水平降低,差異有統(tǒng)計(jì)學(xué)意義(P0.05);與西藥組比較,電針組血CD40L水平無(wú)明顯差異,差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論:1.降低血清中CD40的濃度,對(duì)動(dòng)脈粥樣硬化模型家兔血清中炎癥反應(yīng)起到抑制作用,體現(xiàn)了應(yīng)用電針刺激內(nèi)關(guān)、關(guān)元、足三里穴位的通絡(luò)、活血、化痰功效對(duì)動(dòng)脈粥樣硬化的預(yù)防及治療可能具有積極的意義。2.降低血清中CD40L濃度,對(duì)動(dòng)脈粥樣硬化模型家兔血清中炎癥反應(yīng)起到抑制作用,體現(xiàn)了應(yīng)用電針刺激內(nèi)關(guān)、關(guān)元、足三里穴位的通絡(luò)、活血、化痰功效對(duì)動(dòng)脈粥樣硬化的預(yù)防及治療可能具有積極的意義。
[Abstract]:Objective: to observe the intervention effect of electroacupuncture at Neiguan, Guanyuan and Zusanli on atherosclerosis model rabbits, and to investigate the expression of CD40T CD40L in serum of each group, and to explore the effect of electroacupuncture on Neiguan and Guanyuan. The mechanism of Zusanli (Zusanli) on inhibiting inflammatory response in atherosclerotic rabbits. Materials and methods: Twenty-six healthy male New Zealand rabbits (2-2.5kg / 2 ~ (-3) months old) were randomly divided into normal group (n = 7) and model group (n = 19) after a week of adaptive feeding. The normal group was fed with normal diet, the model group was fed with high fat diet and common feed, and the model group was treated with carotid artery balloon injury after 4 weeks of high fat feeding. After 8 weeks of high fat feeding, one rabbit was randomly selected from the normal group and the model group to detect whether the model was successful or not, and the pathological morphology of carotid atherosclerotic plaque was observed by HE staining. The model group (n = 18) was randomly divided into three groups: model group, electroacupuncture group and western medicine group. In the electroacupuncture group, Neiguan (bilateral and Zusanli) was treated once a day, and the western medicine group was treated with 1mg/kg/d, which was administered by oral administration of Atto calcareous tablets for 6 days, with 1 course of rest between the two courses of treatment for 4 courses. At the end of the fourth course of treatment, all rabbits were killed. Blood was collected from the meridians of the ear margin, serum was isolated, and the carotid artery was fixed in 4% paraformaldehyde for 48 hours. The pathological changes of the carotid artery were analyzed. Enzyme linked immunosorbent assay (Elisa) was used to detect CD40 C 40 L in serum. The result is 1: 1. The effect of electroacupuncture at Neiguan, Guanyuan and Zusanli on CD40 in serum: compared with normal group, the expression of CD40 in serum of model group, electroacupuncture group and western medicine group was higher than that of model group, and the difference was statistically significant (P 0.05). Compared with the western medicine group, there was no significant difference in the plasma CD40 level between the electroacupuncture group and the western medicine group, but there was no significant difference between the electroacupuncture group and the western medicine group. The effect of electroacupuncture at Neiguan, Guanyuan and Zusanli on CD40L in serum: compared with normal group, the expression of CD40L in serum of model group, electroacupuncture group and western medicine group was higher than that of model group, and the difference was statistically significant (P 0.05). The serum CD40L level in the electroacupuncture group and the western medicine group was lower than that in the western medicine group (P 0.05), but there was no significant difference in the serum CD40L level between the electroacupuncture group and the western medicine group (P 0.05). Conclusion 1. Lowering the concentration of CD40 in serum can inhibit the inflammatory reaction in the serum of atherosclerotic model rabbits. It reflects the application of electroacupuncture to stimulate Neiguan, Guanyuan, Zusanli acupoints to clear the collaterals and promote blood circulation. Resolving phlegm effect may have positive significance to the prevention and treatment of atherosclerosis. Lowering the concentration of CD40L in serum can inhibit the inflammatory reaction in the serum of atherosclerosis model rabbits. It reflects the stimulation of acupoints of Neiguan, Guanyuan and Zusanli by electroacupuncture, which can activate the blood circulation and the collaterals of the acupoints of Guanyuan, Guanyuan and Zusanli. The effect of resolving phlegm may be of positive significance to the prevention and treatment of atherosclerosis.
【學(xué)位授予單位】:遼寧中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】:碩士
【學(xué)位授予年份】:2017
【分類(lèi)號(hào)】:R245;R-332
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