本文選題：飲食誘導(dǎo) + 肥胖��； 參考：《南方醫(yī)科大學(xué)》2017年博士論文

【摘要】：第一章飲食誘導(dǎo)肥胖復(fù)合牙周炎大鼠模型的建立目的建立肥胖復(fù)合牙周炎大鼠模型方法雄性Sprague-Dawley(SD)大鼠,4周齡,隨機(jī)分為普通飼料組和高脂飼料組。連續(xù)喂養(yǎng)16周,篩選肥胖大鼠30只。隨機(jī)分為肥胖對(duì)照組(10只)和肥胖復(fù)合牙周炎組(簡(jiǎn)稱復(fù)合組,20只)。20周齡時(shí),復(fù)合組大鼠雙側(cè)上頜第二磨牙用3-0慕絲線進(jìn)行牙周結(jié)扎,誘導(dǎo)實(shí)驗(yàn)性牙周炎。結(jié)果(1)喂養(yǎng)至20周齡,高脂飼料組的體重顯著高于普通飼料組(體重大于對(duì)照組15%)。(2)病理檢查發(fā)現(xiàn),復(fù)合組牙齦上皮屏障出現(xiàn)糜爛性破壞,結(jié)合上皮向根方增殖,牙周袋形成,牙槽骨喪失,肥胖復(fù)合牙周炎大鼠模型成功。結(jié)論高脂飲食喂養(yǎng)16周可建立肥胖大鼠模型;肥胖大鼠模型基礎(chǔ)上進(jìn)行牙周絲線結(jié)扎,可建立肥胖復(fù)合牙周炎大鼠模型。第二章實(shí)驗(yàn)性牙周炎對(duì)肥胖大鼠血清C-反應(yīng)蛋白(C-reactive protein,CRP)及胰島素抵抗的影響目的探討實(shí)驗(yàn)性牙周炎對(duì)肥胖大鼠血清CRP及胰島素抵抗隨時(shí)間的變化。方法復(fù)合組及肥胖組在牙周結(jié)扎前、結(jié)扎后1周、4周眼底眶靜脈采血2mL行空腹血糖、胰島素以及糖化血紅蛋白(HbAlc)的測(cè)定,計(jì)算胰島素抵抗指數(shù)(HOMA-IR),胰島β細(xì)胞功能指數(shù)(HOMA-β);ELISA檢測(cè)血清CRP。結(jié)果(1)牙周結(jié)扎后4周,復(fù)合組大鼠的HOMA-IR(30.29±4.77)顯著高于對(duì)照組(21.95±2.97);復(fù)合組大鼠HOMA-β(755.36±98.11)顯著低于肥胖對(duì)照組(913.53±94.50)。(2)牙周結(jié)扎后1周,復(fù)合大鼠的CRP水平(203.78±53.71ng/mL)顯著高于肥胖組(156.37±33.84ng/mL)。結(jié)論(1)實(shí)驗(yàn)性牙周炎加重肥胖大鼠的胰島素抵抗?fàn)顟B(tài),下調(diào)β細(xì)胞功能。(2)實(shí)驗(yàn)性牙周炎影響大鼠血清CRP水平。第三章非手術(shù)牙周治療對(duì)肥胖大鼠血清炎癥因子及胰島素抵抗的影響目的探討非手術(shù)牙周治療對(duì)肥胖大鼠代謝指標(biāo)及炎癥因子的影響。方法復(fù)合組大鼠16只,按體重排序,依次分為兩組,治療組(8只):拆除絲線,牙周沖洗;未治療組(8只):不予拆除牙周絲線。2周后行口服葡萄糖耐量試驗(yàn)(OGTT),眼底眶靜脈采血2mL行空腹血糖、胰島素以及HbA1c的測(cè)定,計(jì)算 HOMA-IR,HOMA-β;ELISA 檢測(cè)血清 CRP。結(jié)果(1)治療組空腹血糖(5.06±0.69mmol/L)低于未治療組(5.61±0.19mmol/L),HOMA-β(974.54±419.37)高于未治療組(645.37±73.47)。(2)治療組 CRP(140.99±32.10ng/mL)低于未治療組(173.43±28.54ng/mL)(3)OGTT中,治療組在60min、120min的血糖值低于未治療組,糖面積(9.62±0.57)低于治療組未治療組(12.59±0.58)。結(jié)論非手術(shù)牙周治療下調(diào)大鼠血清CRP水平,改善胰島β細(xì)胞功能,改善糖耐量。
[Abstract]:Chapter 1 Establishment of Diet-Induced Rats with Obesity and periodontitis objective to establish a rat model of obesity combined periodontitis methods male Sprague-Dawley SD rats were randomly divided into normal diet group and high-fat diet group. Thirty obese rats were selected by continuous feeding for 16 weeks. The rats were randomly divided into obese control group (n = 10) and obese combined periodontitis group (n = 20). At the age of 20 weeks, bilateral maxillary second molars were ligated with 3-0 filaments to induce experimental periodontitis. Results 1) when fed to the age of 20 weeks, the body weight of the high-fat diet group was significantly higher than that of the normal diet group (body weight was higher than that of the control group). Pathological examination showed that the gingival epithelial barrier appeared erosive destruction in the compound group, the epithelium of the composite group proliferated to the root side, and the periodontal bag formed. Alveolar bone loss, obesity and periodontitis rat model was successful. Conclusion Obesity rat model can be established by high-fat diet feeding for 16 weeks, and obesity combined periodontitis rat model can be established by ligation of periodontal filaments on the basis of obese rat model. Chapter 2 effects of experimental periodontitis on serum C-reactive protein (CRP) and insulin resistance in obese rats objective to investigate the changes of serum CRP and insulin resistance in obese rats with experimental periodontitis. Methods 2mL was collected from orbital vein before periodontal ligation and 1 week after ligation in compound group and obesity group. Fasting blood glucose, insulin and HbAlc were measured. Insulin resistance index (HOMA-IRN) and islet 尾 cell function index (尾 -HOMA- 尾) were calculated. Serum CRP was detected by Elisa. Results at 4 weeks after periodontal ligation, the HOMA-IR(30.29 鹵4.77 in the compound group was significantly higher than that in the control group (21.95 鹵2.97), and the HOMA- 尾 -nmL level in the compound group was significantly lower than that in the obese control group (913.53 鹵94.50 ng 路mL-1). The CRP level in the compound group was 203.78 鹵53.71 ngmL) significantly higher than that in the obese group (156.37 鹵84ngmL / mL) 1 week after periodontal ligation. Conclusion (1) Experimental periodontitis exacerbates insulin resistance and down-regulates 尾 -cell function in obese rats.) Experimental periodontitis affects serum CRP level in rats. Chapter 3 effects of nonoperative periodontal therapy on serum inflammatory factors and insulin resistance in obese rats objective to investigate the effects of nonoperative periodontal therapy on metabolic indexes and inflammatory factors in obese rats. Methods Sixteen rats in the compound group were divided into two groups according to their body weight. Eight rats in the treatment group were divided into two groups: removal of filaments and periodontal irrigation; In the untreated group, 8 rats were treated with oral glucose tolerance test (OGTT) after 2 weeks without removal of periodontal filaments, fasting blood glucose, insulin and HbA1c were measured by 2mL collected from orbital veins. Serum CRPs were measured by HOMA-IRMA-HOMA- 尾 -ELISA. Results 1) the fasting blood glucose in the treatment group was 5.06 鹵0.69 mmol / L) lower than that in the untreated group (5.61 鹵0.19 mmol / L), and the glucose area was 9.62 鹵0.57) significantly lower than that in the untreated group (645.37 鹵73.47 mg 路L / L). The CRP(140.99 鹵32.10 ng / mL in the treatment group was lower than that in the untreated group (173.43 鹵28.54ng/mL)(3)OGTT). The blood glucose level in the treatment group was lower than that in the untreated group at 60 min, and the glucose area was 9.62 鹵0.57) lower than that in the untreated group (12.59 鹵0.58). Conclusion Non-operative periodontal therapy can down-regulate serum CRP level, improve islet 尾 cell function and improve glucose tolerance in rats.
【學(xué)位授予單位】：南方醫(yī)科大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2017
【分類號(hào)】：R781.42;R589.2;R-332

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 陳鐵樓;實(shí)驗(yàn)性牙周炎的形成[J];國外醫(yī)學(xué).口腔醫(yī)學(xué)分冊(cè);1994年04期

2 黃世光;王曉歌;潘倩茹;黃博;呂芳麗;葉秀華;楊云珍;郭柳婷;練楚文;;慢性心理應(yīng)激對(duì)大鼠實(shí)驗(yàn)性牙周炎及血清乳酸脫氫酶1的影響[J];中國病理生理雜志;2012年05期

3 唐華;宮蘋;;人骨保護(hù)素質(zhì)粒轉(zhuǎn)染抑制大鼠實(shí)驗(yàn)性牙周炎骨吸收的研究[J];中國口腔種植學(xué)雜志;2009年02期

4 孟祥嬌;于新波;鄧婧;;氫飽和水對(duì)大鼠實(shí)驗(yàn)性牙周炎抗炎作用的初步觀察[J];齊魯醫(yī)學(xué)雜志;2012年04期

5 李婧;婁晉寧;徐寶華;;實(shí)驗(yàn)性牙周炎動(dòng)物模型的研究進(jìn)展[J];中日友好醫(yī)院學(xué)報(bào);2013年01期

6 胡琳，吳蘭雁，，張舉之;糖尿病在大鼠實(shí)驗(yàn)性牙周炎發(fā)生中的作用[J];牙體牙髓牙周病學(xué)雜志;1995年01期

7 魏玲玲;侯萌;王蘋;宋暉;;一氧化碳釋放分子對(duì)大鼠實(shí)驗(yàn)性牙周炎的影響[J];華西口腔醫(yī)學(xué)雜志;2014年01期

8 鄢明明,劉泓,馮占軍,趙爾揚(yáng),劉文珠;實(shí)驗(yàn)性牙周炎雌、孕激素受體免疫組織化學(xué)觀察[J];哈爾濱醫(yī)科大學(xué)學(xué)報(bào);2002年01期

9 廖旭輝;顧為望;徐君逸;;實(shí)驗(yàn)性牙周炎動(dòng)物模型的微生物研究[J];廣東牙病防治;2007年02期

10 劉潁鳳;王小競(jìng);吳禮安;;大鼠尼古丁實(shí)驗(yàn)性牙周炎動(dòng)物模型的建立[J];牙體牙髓牙周病學(xué)雜志;2008年03期

相關(guān)會(huì)議論文 前6條

1 唐華;宮蘋;;人骨保護(hù)素質(zhì)粒轉(zhuǎn)染抑制大鼠實(shí)驗(yàn)性牙周炎骨吸收的研究[A];第六屆全國口腔種植學(xué)術(shù)會(huì)議論文匯編[C];2009年

2 畢良佳;李虹;張瑞敏;林江;姜云濤;于洋;鄧卜師;;實(shí)驗(yàn)性牙周炎合并咬合性外傷時(shí)牙周膜的變化[A];中華口腔醫(yī)學(xué)會(huì)第二次全國會(huì)員代表大會(huì)暨第七次全國口腔醫(yī)學(xué)學(xué)術(shù)會(huì)議論文匯編[C];2001年

3 金惠姣;薛毅;陳光;吳仲寅;;輔酶Q_(10)對(duì)大鼠實(shí)驗(yàn)性牙周炎的影響[A];中華口腔醫(yī)學(xué)會(huì)口腔藥學(xué)專業(yè)委員會(huì)第二次全國口腔藥學(xué)學(xué)術(shù)會(huì)議論文匯編[C];2013年

4 丁鰲;張志宏;王勤濤;;IL-17抗體在動(dòng)物實(shí)驗(yàn)性牙周炎中作用的研究[A];第十次全國牙周病學(xué)學(xué)術(shù)會(huì)議論文摘要匯編[C];2014年

5 楊恒;R.Aprecio;周小東;王琪;張武;丁一;李一鳴;;多功能誘導(dǎo)干細(xì)胞來源MSCs經(jīng)TSG-6修飾后對(duì)實(shí)驗(yàn)性牙周炎的抗炎作用評(píng)價(jià)[A];第十次全國牙周病學(xué)學(xué)術(shù)會(huì)議論文摘要匯編[C];2014年

6 馬衛(wèi)劍;彭偉;岳亞瑞;李亞楠;;葛根芩連湯對(duì)實(shí)驗(yàn)性牙周炎大鼠治療前后血清及牙周組織中IL-1β、IL-6、TNF-α的影響[A];第十次全國牙周病學(xué)學(xué)術(shù)會(huì)議論文摘要匯編[C];2014年

相關(guān)博士學(xué)位論文 前4條

1 龔愛秀;活性維生素D在防治牙周炎中的作用及機(jī)制研究[D];南京醫(yī)科大學(xué);2016年

2 鐘素蘭;實(shí)驗(yàn)性牙周炎及非手術(shù)牙周治療對(duì)肥胖大鼠血清炎癥因子和胰島素抵抗影響的研究[D];南方醫(yī)科大學(xué);2017年

3 馬守治;人白細(xì)胞介素-10基因治療對(duì)去卵巢大鼠實(shí)驗(yàn)性牙周炎發(fā)生的影響[D];福建醫(yī)科大學(xué);2011年

4 唐華;人骨保護(hù)素質(zhì)粒轉(zhuǎn)染抑制大鼠實(shí)驗(yàn)性牙周炎骨吸收的研究[D];四川大學(xué);2007年

相關(guān)碩士學(xué)位論文 前10條

1 孟明理;抗牙齦素粘附片段Hgp44卵黃抗體對(duì)實(shí)驗(yàn)性牙周炎的抑制作用[D];安徽醫(yī)科大學(xué);2016年

2 黃捷;云南煙葉提取物輔酶Q10對(duì)實(shí)驗(yàn)性牙周炎抗炎抗氧化的機(jī)制研究[D];昆明醫(yī)科大學(xué);2016年

3 易湘豪;大鼠實(shí)驗(yàn)性牙周炎齦組織中堿性磷酸酶活性的研究[D];蘭州大學(xué);2009年

4 孟祥嬌;氫飽和水對(duì)大鼠實(shí)驗(yàn)性牙周炎抗炎作用的初步觀察[D];青島大學(xué);2012年

5 孫艷楠;吸煙大鼠實(shí)驗(yàn)性牙周炎動(dòng)物模型的建立[D];青島大學(xué);2011年

6 趙雅娟;心理應(yīng)激對(duì)大鼠實(shí)驗(yàn)性牙周炎預(yù)后及牙周膜堿性成纖維細(xì)胞生長因子表達(dá)影響的研究[D];第四軍醫(yī)大學(xué);2012年

7 吳云鼎;蜂膠對(duì)犬實(shí)驗(yàn)性牙周炎四種主要致病菌抑菌作用的實(shí)驗(yàn)研究[D];昆明醫(yī)科大學(xué);2013年

8 郭小紅;大黃緩釋膜治療實(shí)驗(yàn)性牙周炎的研究[D];蘭州大學(xué);2009年

9 靳趁心;骨保護(hù)素與去卵巢實(shí)驗(yàn)性牙周炎大鼠模型的實(shí)驗(yàn)研究[D];天津醫(yī)科大學(xué);2006年

10 廉成眾;雌激素缺乏狀態(tài)及雌激素治療對(duì)大鼠實(shí)驗(yàn)性牙周炎骨吸收的影響[D];山西醫(yī)科大學(xué);2006年

本文編號(hào)：1791567