本文選題：尖葉假龍膽 + 類風(fēng)濕關(guān)節(jié)炎　； 參考：《黑龍江中醫(yī)藥大學(xué)》2017年碩士論文

【摘要】：目的:以尖葉假龍膽為研究對象,通過尖葉假龍膽對佐劑型關(guān)節(jié)炎模型大鼠的藥效學(xué)實(shí)驗(yàn)。方法:以佐劑性關(guān)節(jié)炎模型大鼠,將SD大鼠隨機(jī)分為模型組、空白組、陽性組(雷公藤多苷)、尖葉假龍膽高劑量組、尖葉假龍膽中劑量組、尖葉假龍膽低劑量組,每組8只。除空白組外在其余大鼠右后足足跖皮下注射弗氏完全佐劑(CFA)0.1ml致炎,復(fù)制模擬AA模型,空白組用生理鹽水同法注射。從第14天開始灌胃,連續(xù)灌胃18天。定期測量大鼠體重,右足足趾腫脹度,踝關(guān)節(jié)和脾臟病理HE,ELISA法檢測大鼠血清中的TNF-α、IL-10含量,免疫組化法檢測滑膜組織里的VEGF、ICAM-1、MMP-3蛋白表達(dá)。結(jié)果:1尖葉假龍膽各劑量組可以增加AA大鼠的體重、減少AA大鼠足趾的腫脹、降低AA大鼠關(guān)節(jié)炎評分、降低AA大鼠脾臟白髓的生成。2.尖葉假龍膽各劑量組可以通過降低AA大鼠血清中的TNF-α的含量及提升血清IL-10的含量,以達(dá)到緩解佐劑性關(guān)節(jié)炎的作用。(P0.05)3.尖葉假龍膽各劑量組可以降低AA大鼠滑膜組織VEGF、ICAM-1、MMP3蛋白表達(dá),從而達(dá)到緩解滑膜炎癥、減輕關(guān)節(jié)腫脹、延緩關(guān)節(jié)骨破壞的目的。其中高劑量組效果最明顯。(P0.05,P0.01)結(jié)論:尖葉假龍膽能有效的抑制繼發(fā)性關(guān)節(jié)腫脹,可顯著降低AA大鼠血清中的TNF-α的含量及提升血清IL-10的含量,對滑膜組織ICAM-1、VEGF、MMP3蛋白表達(dá)調(diào)控作用達(dá)到緩解滑膜炎癥、減輕關(guān)節(jié)腫脹、延緩關(guān)節(jié)骨破壞的病理基礎(chǔ)。綜合來看尖葉假龍膽高劑量組優(yōu)于中劑量組和低劑量組。
[Abstract]:Aim: to study the pharmacodynamics of pseudogentian on adjuvant arthritis rats.Methods: rats with adjuvant arthritis were randomly divided into three groups: model group, blank group, positive group (Tripterygium wilfordii, high dose group, middle dose group, low dose group, 8 rats in each group).In addition to the blank group, the other rats were subcutaneously injected with CFAA 0.1ml, a complete adjuvant of CFAA, to induce inflammation, and the model of AA was reproduced. The blank group was injected with normal saline in the same way.From day 14 to day 14, gavage lasted for 18 days.The weight of rats, the swelling degree of right foot and toe, the content of TNF- 偽 IL-10 in rat serum and the expression of VEGFICAM-1 MMP-3 in synovial tissue were detected by Elisa.Results the weight of AA rats was increased, the swelling of toes, arthritis score of AA rats and the formation of white pulp in spleen of AA rats were decreased.In order to alleviate adjuvant arthritis, different dose groups of Pseudogentis auriculata could reduce the content of TNF- 偽 in serum and increase the content of serum IL-10 in AA rats, so as to relieve adjuvant arthritis.The expression of VEGFU ICAM-1 MMP3 protein in synovial tissue of AA rats could be reduced in different dose groups of pseudogentian, so as to relieve synovitis, alleviate joint swelling and delay joint bone destruction.Conclusion: the apical leaf pseudogentian can effectively inhibit the secondary joint swelling, decrease the content of TNF- 偽 in serum and increase the content of serum IL-10 in AA rats.By regulating the expression of ICAM-1 VEGFN MMP3 protein in synovial tissue, the pathological basis of relieving synovitis, reducing joint swelling and delaying joint bone destruction can be achieved.In a word, the high dose group was superior to the middle dose group and the low dose group.
【學(xué)位授予單位】：黑龍江中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R285.5;R-332

【相似文獻(xiàn)】

相關(guān)期刊論文 前4條

1 王知斌;翟亞東;于瑩;宋璞原;王秋紅;楊炳友;匡海學(xué);;尖葉假龍膽中木脂素類成分的分離與鑒定[J];中藥材;2014年05期

2 魏茜;劉銅華;時曉娟;李朋收;魏穎;徐暾海;;HPLC法測定尖葉假龍膽中3個[口山]酮苷的含量[J];世界科學(xué)技術(shù)-中醫(yī)藥現(xiàn)代化;2014年05期

3 龍平;李e，

本文編號：1749428