本文選題：潤燥解毒通絡(luò)法　切入點：干燥綜合征　出處：《浙江中醫(yī)藥大學(xué)》2017年碩士論文

【摘要】：目的研究潤燥解毒通絡(luò)法中藥對干燥綜合征(sjogren syndrome,SS)小鼠模型血清及頜下腺毒蕈堿樣乙酰膽堿3型(muscarinic acethyleholine receptor 3,M3R)表達(dá)的影響,以探討潤燥解毒通絡(luò)法中藥(解毒通絡(luò)生津湯)治療SS的作用機(jī)理。方法選用BALB/c小鼠47只,隨機(jī)分為正常對照組(正常組,n=10)和造模組(n=37)。取BALB/c小鼠頜下腺制備抗原進(jìn)行造模。造模組采用背部皮內(nèi)5個部位多點注射抗原的方法進(jìn)行免疫,建立SS模型。造模成功后隨機(jī)分為模型組(n=10)、中藥組(n=10)和HCQ組(n=10)。分別灌胃給藥:中藥組按11.4g/kg/d劑量灌胃中藥解毒通絡(luò)生津湯,模型組灌胃等量生理鹽水,HCQ組小鼠按0.06g/kg/d劑量灌胃HCQ混懸液(HCQ與生理鹽水混合液),均每日1次,正常組正常飼養(yǎng)。同時測定小鼠唾液量、飲水量。連續(xù)用藥30天后處死,取各組小鼠頜下腺、脾及血清。采用蘇木素-伊紅(HE)染色評價頜下腺淋巴細(xì)胞浸潤情況,采用ELISA法測定標(biāo)本血清M3R水平,免疫組化法檢測頜下腺M3R表達(dá)水平。結(jié)果1、造模成功后治療前,與正常組比較,模型組、中藥組和HCQ組小鼠唾液量減少,差異均有統(tǒng)計學(xué)意義(P0.05);模型組、中藥組、HCQ組之間比較,小鼠唾液量比較差異無統(tǒng)計學(xué)意義(P0.05)。治療第30天,與正常組比較,模型組唾液量減少,差異有統(tǒng)計學(xué)意義(P0.05);與模型組比較,中藥組、HCQ組小鼠唾液量增加,差異均有統(tǒng)計學(xué)意義(P0.05);中藥組和HCQ組之間比較差異無統(tǒng)計學(xué)意義(P0.05)。與本組治療前比較,治療后中藥組、HCQ組小鼠唾液量增加,差異均有統(tǒng)計學(xué)意義(P0.05)。2、與正常組比較,模型組小鼠血清M3R表達(dá)增加,差異有統(tǒng)計學(xué)意義(P0.05);與模型組比較,中藥組、HCQ組M3R表達(dá)減少,差異有統(tǒng)計學(xué)意義(P0.05)。中藥組和HCQ組之間比較,差異無統(tǒng)計學(xué)意義(P0.05)。3、與正常組比較,模型組小鼠頜下腺M3R表達(dá)增加,差異有統(tǒng)計學(xué)意義(P0.01);與模型組比較,中藥組、HCQ組小鼠頜下腺M3R表達(dá)減少,差異有統(tǒng)計學(xué)意義(P0.05)。中藥組和HCQ組之間比較,差異無統(tǒng)計學(xué)意義(P0.05)。4、與正常組比較,模型組小鼠頜下腺淋巴細(xì)胞浸潤明顯,差異有統(tǒng)計學(xué)意義(P0.01);與模型組比較,中藥組和HCQ組小鼠頜下腺淋巴細(xì)胞浸潤減輕,差異有統(tǒng)計學(xué)意義(P0.05);中藥組和HCQ組之間比較,差異無統(tǒng)計學(xué)意義(P0.05)。5、各組之間脾指數(shù)、頜下腺指數(shù)比較,差異均無統(tǒng)計學(xué)意義(P0.05)。結(jié)論SS小鼠血清及頜下腺M3R表達(dá)水平升高。潤燥解毒通絡(luò)法中藥可能通過下調(diào)M3R表達(dá),從而抑制頜下腺淋巴細(xì)胞浸潤,改善腺體分泌能力,阻止SS進(jìn)展。
[Abstract]:Objective to study the effect of moistening and detoxifying and removing collaterals on the expression of muscarinic acethyleholine receptor 3 M 3R in serum and submandibular gland of Sjogren syndrome (Sjogren syndromes) mouse model.To explore the mechanism of moistening dryness and detoxification and dredging collaterals (detoxifying and releasing collaterals Shengjin decoction) in treating SS.Methods 47 BALB/c mice were randomly divided into normal control group (normal group) and model group.The submandibular glands of BALB/c mice were taken to prepare antigens for modeling.The model group was immunized by multiple injection of antigens in 5 parts of the back skin, and SS model was established.The models were randomly divided into two groups: model group (n = 10), Chinese medicine group (n = 10) and HCQ group (n = 10).The rats in the model group were treated with the same amount of normal saline. The mice in the model group were fed with the mixture of HCQ and normal saline at the same dose of 0.06g/kg/d, all of them were fed once a day, and the normal control group was fed normally.At the same time, the amount of saliva and drinking water were determined.After 30 days of continuous administration, the mice were killed and the submandibular gland, spleen and serum were taken.The submandibular gland lymphocytic infiltration was evaluated by hematoxylin and eosin (HEH) staining, the serum M3R level was measured by ELISA method, and the expression of M3R in submandibular gland was detected by immunohistochemical method.Results 1. Before treatment, the saliva quantity of model group, traditional Chinese medicine group and HCQ group decreased significantly compared with normal group, and there was no significant difference between model group and traditional Chinese medicine group (P 0.05).On the 30th day, compared with the normal group, the saliva quantity in the model group decreased, the difference was statistically significant (P 0.05), compared with the model group, the saliva quantity in the HCQ group increased.The difference was statistically significant (P 0.05), but there was no significant difference between traditional Chinese medicine group and HCQ group (P 0.05).Compared with the control group, the saliva content of the HCQ group increased after treatment, and the difference was statistically significant (P 0.05). Compared with the normal group, the serum M3R expression in the model group was higher than that in the normal group (P 0.05), and that in the model group was significantly higher than that in the model group.The expression of M3R in HCQ group was significantly lower than that in HCQ group (P 0.05).Compared with the normal group, the expression of M3R in the submandibular gland of the model group was increased, the difference was statistically significant (P 0.01), and the expression of M3R in the submandibular gland of the Chinese medicine group was lower than that of the model group, while the expression of M3R in the submandibular gland of the model group was significantly lower than that in the control group.The difference was statistically significant (P 0.05).There was no significant difference between the traditional Chinese medicine group and the HCQ group, and there was no significant difference between the two groups. Compared with the normal group, the submandibular gland lymphocytic infiltration in the model group was obvious, the difference was statistically significant (P 0.01), and that in the model group was higher than that in the model group.The lymphocyte infiltration of submandibular gland in Chinese medicine group and HCQ group was alleviated, the difference was statistically significant (P 0.05), but there was no significant difference between traditional Chinese medicine group and HCQ group. There was no significant difference in spleen index and submandibular gland index between each group.Conclusion the expression of M 3 R in serum and submandibular gland of SS mice was increased.The method of moistening dryness detoxification and dredging collaterals may inhibit the lymphocyte infiltration of submandibular gland improve the secretory ability of glands and prevent the progression of SS by down-regulating the expression of M3R.
【學(xué)位授予單位】：浙江中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R285.5;R-332

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