本文選題：臍帶間充質(zhì)干細(xì)胞　切入點(diǎn)：脂肪間充質(zhì)干細(xì)胞　出處：《生物工程學(xué)報(bào)》2013年11期

【摘要】：為研究1-磷酸鞘氨醇(Sphingosine-1-phosphate,S1P)對(duì)臍帶間充質(zhì)干細(xì)胞(Umbilical cord mesenchymal stem cells,UC-MSCs)和脂肪間充質(zhì)干細(xì)胞(Adipose derived mesenchymal stem cells,AD-MSCs)向心肌分化的影響,探索其適宜的作用時(shí)間和濃度,將UC-MSCs和AD-MSCs接種到培養(yǎng)板,用添加不同濃度S1P的心肌細(xì)胞培養(yǎng)液誘導(dǎo)兩種干細(xì)胞向心肌分化,誘導(dǎo)時(shí)間分為7 d、14 d和28 d。采用免疫熒光染色檢測(cè)心肌特異性蛋白,α-肌動(dòng)蛋白(α-actin)、縫隙連接蛋白(Connexin-43)以及肌球蛋白重鏈(MYH-6)的表達(dá),并通過(guò)共聚焦顯微鏡和熒光顯微鏡進(jìn)行觀察;采用MTT分析細(xì)胞的活性;膜片鉗檢測(cè)分化細(xì)胞的鈣瞬變(此為心肌細(xì)胞的功能性指標(biāo))。結(jié)果表明,S1P與心肌細(xì)胞培養(yǎng)液協(xié)同作用,能夠促進(jìn)UC-MSCs和AD-MSCs向心肌細(xì)胞的分化。并且,隨著S1P濃度的增加,促分化作用增強(qiáng),但細(xì)胞活性降低。S1P在心肌細(xì)胞培養(yǎng)液中的適宜作用時(shí)間為14 d,適宜作用濃度為0.5μmol/L。而且聯(lián)合心肌細(xì)胞培養(yǎng)液可以使UC-MSCs和AD-MSCs的心肌分化細(xì)胞產(chǎn)生鈣瞬變,具有類(lèi)似心肌細(xì)胞的功能性。S1P能夠與心肌細(xì)胞培養(yǎng)液協(xié)同作用,促進(jìn)UC-MSCs和AD-MSCs的心肌功能性分化。
[Abstract]:In order to study the effects of Sphingosine-1-phosphate S1P on myocardial differentiation of umbilical cord mesenchymal stem cells (Umbilical cord mesenchymal stem stem cells) and adipose mesenchymal stem cells (adipose derived mesenchymal stem cells AD-MSCs), and to explore the appropriate time and concentration of Sphingosine-1-phosphateanine, UC-MSCs and AD-MSCs were inoculated into the culture plate. Two kinds of stem cells were induced to differentiate into myocardium by adding different concentrations of S1P into cardiomyocyte culture medium. The induction time was divided into 7 days, 14 days and 28 days. The expression of myocardial specific protein, 偽 -actin (偽 -actin), gap junction protein (gap junction protein) and myosin heavy chain myosin (MYH-6) were detected by immunofluorescence staining, and were observed by confocal microscope and fluorescence microscope. MTT was used to analyze cell activity, patch clamp was used to detect calcium transient of differentiated cells (this is the functional index of cardiomyocytes). The results showed that S1P could promote the differentiation of UC-MSCs and AD-MSCs into cardiomyocytes by synergistic action with cardiomyocyte culture medium. With the increase of S1P concentration, the effect of promoting differentiation was enhanced. However, the suitable time for the decrease of S1P activity in cardiomyocyte culture medium was 14 days and the optimal concentration was 0.5 渭 mol / L. moreover, combined with cardiomyocyte culture medium, calcium transient was produced in myocardial differentiation cells of UC-MSCs and AD-MSCs. S1P, which is similar to cardiomyocytes, can promote myocardial functional differentiation of UC-MSCs and AD-MSCs by synergistic action with cardiomyocyte culture medium.
【作者單位】： 大連理工大學(xué)化工學(xué)院干細(xì)胞與組織工程研發(fā)中心;大連理工大學(xué)盤(pán)錦校區(qū)生命與醫(yī)藥學(xué)院;
【基金】：國(guó)家自然科學(xué)基金(No.31170945)資助~~
【分類(lèi)號(hào)】：R329

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 韓正濱;陳紅星;鄧?yán)^先;;脂肪源性干細(xì)胞的多向分化潛力及應(yīng)用前景[J];生物工程學(xué)報(bào);2007年02期

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 李宏松;鄒俊;;脂肪干細(xì)胞在眼科的應(yīng)用及研究進(jìn)展[J];國(guó)際眼科雜志;2013年11期

2 趙宏偉;怡榮;那仁格日勒;高錦;陳曦;阿拉坦高勒;;鞘氨醇-1-磷酸對(duì)人臍帶間充質(zhì)干細(xì)胞增殖及表面標(biāo)記物表達(dá)的影響[J];中國(guó)生物化學(xué)與分子生物學(xué)報(bào);2012年05期

3 Ying Hu;Jun Liang;Hongping Cui;Xinmei Wang;Hua Rong;Bin Shao;Hao Cui;;Wharton's jelly mesenchymal stem cells differentiate into retinal progenitor cells[J];Neural Regeneration Research;2013年19期

4 李珊;梁戰(zhàn)華;劉晶;;銀杏葉提取物對(duì)人脂肪間充質(zhì)干細(xì)胞神經(jīng)向分化的影響[J];神經(jīng)解剖學(xué)雜志;2013年05期

5 郭守東;于楊;馮蕾;張穎;崔英杰;秦樹(shù)存;;液相串聯(lián)質(zhì)譜法同時(shí)定量檢測(cè)生物樣本中鞘脂類(lèi)化合物[J];中國(guó)生物化學(xué)與分子生物學(xué)報(bào);2013年11期

6 趙振強(qiáng);陳志斌;蔡美華;王淑榮;陳蓉;王W，

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