發(fā)布時(shí)間：2018-03-24 00:27

  本文選題：銅綠假單胞菌　切入點(diǎn)：氨基糖苷類(lèi)　出處：《中華醫(yī)院感染學(xué)雜志》2013年06期

【摘要】：目的研究泛耐藥銅綠假單胞菌對(duì)氨基糖苷類(lèi)的耐藥機(jī)制,以指導(dǎo)臨床合理使用抗菌藥物,降低細(xì)菌耐藥性的產(chǎn)生。方法收集2010年1-12月浙江紹興第二醫(yī)院住院患者痰液標(biāo)本中分離到的泛耐藥銅綠假單胞菌共20株,用聚合酶鏈反應(yīng)(PCR)及序列分析的方法分析14種氨基糖苷類(lèi)修飾酶基因與7種16SrRNA甲基化酶基因。結(jié)果 20株銅綠假單胞菌均檢測(cè)到aac(6′)-Ⅱ及rmtB基因,檢出率100.0%;17株檢測(cè)到aac(6′)-Ⅰb,檢出率85.0%;18株檢測(cè)到ant(2″)-Ⅰ,檢出率90.0%;2株檢測(cè)到ant(3″)-Ⅰ,檢出率10.0%。結(jié)論 aac(6′)-Ⅱ型氨基糖苷類(lèi)修飾酶與rmtB型16SrRNA甲基化酶基因流行是泛耐藥銅綠假單胞菌對(duì)氨基糖苷類(lèi)耐藥的重要原因。
[Abstract]:Objective to study the resistance mechanism of pan-resistant Pseudomonas aeruginosa to aminoglycosides and to guide the rational use of antimicrobial agents in clinic. Methods A total of 20 strains of pan-resistant Pseudomonas aeruginosa were isolated from sputum samples of hospitalized patients in Shaoxing Hospital of Zhejiang Province from January to December 2010. 14 aminoglycoside modified enzyme genes and 7 16SrRNA methylase genes were analyzed by polymerase chain reaction (PCR) and sequence analysis. The detectable rate was 100.0%. The detectable rate was 85.0%. The detection rate was 85.0%. The detectable rate was 90.0%. The detection rate was 90.0%. The detection rate was 90.010%, and the detection rate was 90.0%, and the detection rate was 85.0% and 85.0% respectively, and the detection rate was 90.0%. Conclusion the prevalence of aminoglycoside modifying enzyme type 鈪，

本文編號(hào)：1655903