本文選題：銅綠假單胞菌　切入點：氨基糖苷類　出處：《中華醫(yī)院感染學雜志》2013年06期

【摘要】：目的研究泛耐藥銅綠假單胞菌對氨基糖苷類的耐藥機制,以指導臨床合理使用抗菌藥物,降低細菌耐藥性的產(chǎn)生。方法收集2010年1-12月浙江紹興第二醫(yī)院住院患者痰液標本中分離到的泛耐藥銅綠假單胞菌共20株,用聚合酶鏈反應(PCR)及序列分析的方法分析14種氨基糖苷類修飾酶基因與7種16SrRNA甲基化酶基因。結(jié)果 20株銅綠假單胞菌均檢測到aac(6′)-Ⅱ及rmtB基因,檢出率100.0%;17株檢測到aac(6′)-Ⅰb,檢出率85.0%;18株檢測到ant(2″)-Ⅰ,檢出率90.0%;2株檢測到ant(3″)-Ⅰ,檢出率10.0%。結(jié)論 aac(6′)-Ⅱ型氨基糖苷類修飾酶與rmtB型16SrRNA甲基化酶基因流行是泛耐藥銅綠假單胞菌對氨基糖苷類耐藥的重要原因。
[Abstract]:Objective to study the resistance mechanism of pan-resistant Pseudomonas aeruginosa to aminoglycosides and to guide the rational use of antimicrobial agents in clinic. Methods A total of 20 strains of pan-resistant Pseudomonas aeruginosa were isolated from sputum samples of hospitalized patients in Shaoxing Hospital of Zhejiang Province from January to December 2010. 14 aminoglycoside modified enzyme genes and 7 16SrRNA methylase genes were analyzed by polymerase chain reaction (PCR) and sequence analysis. The detectable rate was 100.0%. The detectable rate was 85.0%. The detection rate was 85.0%. The detectable rate was 90.0%. The detection rate was 90.0%. The detection rate was 90.010%, and the detection rate was 90.0%, and the detection rate was 85.0% and 85.0% respectively, and the detection rate was 90.0%. Conclusion the prevalence of aminoglycoside modifying enzyme type 鈪，

本文編號：1655903