本文選題：衰老　切入點：埋線　出處：《廣州中醫(yī)藥大學》2017年碩士論文　論文類型：學位論文

【摘要】：目的衰老是由于生物體隨著時間的流逝而在結構和機能上出現(xiàn)衰退,在適應能力和抵抗能力上出現(xiàn)降低的現(xiàn)象。由于當今生活環(huán)境不斷改善,人類壽命逐步延伸,使得整個人類社會日趨老齡化,以致研究如何"抗衰老"的意義日漸凸顯。本實驗通過對健康大鼠注射D-半乳糖溶液合并制動應激處理,欲制造出貼近由于內外因素共同作用致衰的人類自然衰老模型,將大鼠行為學,胸腺指數(shù)以及與神經-內分泌-免疫系統(tǒng)(HPA軸)的相關激素作為檢測指標,觀察五臟背俞穴埋線對大鼠一般行為學、胸腺指數(shù)、血清皮質醇(Cor)、血漿促腎上腺皮質激素(ATCH)以及下丘腦促腎上腺皮質激素釋放激素(CRH)的影響,綜合闡述埋線對衰老機體的調節(jié)作用,以便為日后相關抗衰實驗提供理論依據。方法SPF級,健康3月齡,雄性SD大鼠60只,體重200-250g。以體重作為分層條件,隨機區(qū)組設計分為6組:空白對照組(A組)、D-半乳糖組(B組)、D-半乳糖+埋線組(C組)、D-半乳糖+應激組(D組)、D-半乳糖+應激+埋線組(E組),每組12只。每日給予B組、C組、D組和E組大鼠腹腔注射D-半乳糖溶液,A組則給予腹腔注射等量的生理鹽水,共8周。D組和E組于第五周開始,給予制動應激,連續(xù)應激28日。從實驗開始時即每日觀察大鼠的精神狀態(tài)、毛發(fā)形態(tài),并稱量記錄大鼠體重。8周后,各組大鼠主動脈采血,分離血清、血漿,運用酶聯(lián)免疫吸附反應法(ELISA)檢測各組大鼠Cor、ATCH含量。并斷頭開顱,取出下丘腦,組織勻漿,取上清液,運用酶聯(lián)免疫吸附反應法(ELISA)檢測CRH含量。最后,取出胸腺,測量胸腺指數(shù)。運用統(tǒng)計學方法對比各組實驗數(shù)據,采用SPSS22.0統(tǒng)計軟件進行統(tǒng)計學處理。結果1.A組大鼠體重增長最多(P0.05),B組次之,C組次之,E組次之,D組最少(P值均0.05);2.A組大鼠胸腺指數(shù)最大(P0.05),B組次之,C組次之,E組次之,D組最少(P值均0.05);3.D組大鼠Cor、ATCH、CRH均處于較高范疇(P0.05),E組次之,B組次之,C組次之,A組最少(P值均0.05),差異具有統(tǒng)計學意義。結論1.五臟背俞穴埋線可以改善機體在衰老過程中所出現(xiàn)的體重減輕、精神低落、毛發(fā)枯黃等一般行為學表現(xiàn)。2.五臟背俞穴埋線對于機體衰老過程中所出現(xiàn)的胸腺萎縮狀態(tài),具有一定的改善作用。3.五臟背俞穴埋線對于機體衰老過程中所出現(xiàn)的HPA軸功能亢進,具有一定的抑制作用。
[Abstract]:Objective Aging is a decline in the structure and function of organisms over time and a decrease in their ability to adapt and resist. As a result of the continuous improvement of the living environment today, the life span of human beings is gradually extending, As a result of the aging of the whole human society, the significance of the study on how to "resist aging" becomes more and more prominent. This experiment was done by injecting D- galactose solution into healthy rats combined with immobilization stress. In order to create a human model of natural aging, which was induced by internal and external factors, rat behavior, thymus index and related hormones related to the neuroendocrine and immune system (HPA axis) were used as indicators. To observe the effects of embedding thread on general behavior, thymus index, serum cortisol Corn, plasma adrenocorticotropic hormone (ACTH) and hypothalamic corticotropin releasing hormone (CRH) in rats. In order to provide theoretical basis for related anti-aging experiments in the future, 60 male SD rats of SPF grade, healthy 3 months old and weight of 200-250 g were used as stratification conditions. The randomized block design was divided into 6 groups: control group A, group A, group A, group B, group B, group B, group B, group B, group C, group C, group C, group C, group C, group D, group D, group D, group D, group D, group D, group E, group E, catgut embedding with D-galactose stress, group E, 12 rats in each group, given daily to group B, with 12 rats in each group. Rats in group C, group D and group E were injected intraperitoneally with Dgalactose solution. Group A was given intraperitoneal injection of the same amount of normal saline. The rats in group D and group E were given immobilization stress at 5th weeks and continuous stress on 28th. The mental state and hair morphology of rats were observed daily at the beginning of the experiment, and the blood samples were collected from aorta of each group after weighing and recording the weight of rats. Serum and plasma were isolated, and the content of Coranach was detected by Elisa. The hypothalamus, homogenate and supernatant were removed from hypothalamus and supernatant, and the content of CRH was detected by enzyme linked immunosorbent assay (Elisa). Finally, the contents of CRH were determined by enzyme-linked immunosorbent assay (Elisa). The thymus gland was taken out, the thymus index was measured, and the experimental data of each group were compared by statistical method. Results 1. Weight gain of rats in group A was the highest in group B and group B was followed by group C, followed by group E, followed by group D and the lowest P value. 2. Group A, group A, rats with the largest thymus index, group B, group C, group C, group E, group D, second group, group D, group D, the second group. The minimum P values of group D were 0.05 and 0.05 respectively. The CRH of group D was in the higher category. Group B, group B, group C, group C, followed by group A, the minimum P values were 0.050.Conclusion 1.The embedding of the points at Wuzhangbackshu can improve the body's aging process. 2. The weight loss that occurs, General behavioral manifestation of low spirits, yellow hair, etc. 2.The state of thymus atrophy that occurs in the aging process of the body by embedding wires at the point of the back of the five viscera, It can improve the function of HPA axis and inhibit the function of HPA axis in the aging process. 3. The buried thread at Wuzangbushu point can inhibit the function of HPA axis in the process of aging.
【學位授予單位】：廣州中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R245;R-332

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