本文選題：傷害性感覺神經(jīng)元　切入點(diǎn)：酪氨酸激酶A(trkA)　出處：《深圳大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:機(jī)體的感覺神經(jīng)節(jié)主要包括三叉神經(jīng)節(jié)(trigeminal ganglion,TRG)和背根神經(jīng)節(jié)(dorsal root ganglion,DRG),均由多種不同類型的感覺神經(jīng)元組成,感覺神經(jīng)元將特定的刺激轉(zhuǎn)化成動(dòng)作電位或分級(jí)電位,將感覺信息傳送到腦或脊髓,其中包括了負(fù)責(zé)疼痛感受的傷害性感覺神經(jīng)元,它們傳遞組織損傷或應(yīng)對(duì)威脅的信號(hào),是機(jī)體實(shí)現(xiàn)自我保護(hù)的重要途徑。迄今為止,文獻(xiàn)中缺少關(guān)于不同發(fā)育年齡TRG中傷害性感覺神經(jīng)元組分及變化規(guī)律的報(bào)道,因此本實(shí)驗(yàn)旨在探討大鼠TRG中傷害性感覺神經(jīng)元在不同發(fā)育年齡的變化及其規(guī)律,并與大鼠背根神經(jīng)節(jié)DRG中傷害性感覺神經(jīng)元構(gòu)成情況進(jìn)行定量比較分析。本實(shí)驗(yàn)?zāi)軌蛱钛a(bǔ)科研文獻(xiàn)上的資料空缺,為疼痛及相關(guān)領(lǐng)域研究提供依據(jù)和參考。方法:選取不同發(fā)育年齡階段的Sprague-Dawley(SD)大鼠,每個(gè)年齡組(出生后第0,3,7,14,21,60天)6只,未成年大鼠經(jīng)麻醉后處死,成年大鼠則在局麻后進(jìn)行心臟灌流,然后取其右側(cè)的TRGs,成年大鼠同時(shí)從背部取同側(cè)腰4、5、6節(jié)段(L4、L5、L6)的DRGs,所有組織立即分別浸泡于4%多聚甲醛中進(jìn)行固定,經(jīng)進(jìn)一步脫水、透明和浸蠟后,制備成石蠟切片。用抗酪氨酸激酶A抗體(anti-tyrosine kinase Aantibody,trkA-Ab)和植物凝集素B4(Isolectin-B4,IB4)對(duì)切片中所有傷害性感覺神經(jīng)元進(jìn)行免疫熒光染色,并計(jì)算各類陽性標(biāo)記神經(jīng)元占神經(jīng)節(jié)中全部感覺神經(jīng)元的比率,定量數(shù)據(jù)均用均數(shù)±標(biāo)準(zhǔn)誤進(jìn)行表述,并進(jìn)行統(tǒng)計(jì)學(xué)分析,p值小于0.05代表差異具有顯著性。結(jié)果:從大鼠出生當(dāng)天至成年,三叉神經(jīng)節(jié)中表達(dá)trkA的神經(jīng)元占整體神經(jīng)元比率分別為 69.51±2.02%、60.15±1.38%、53.87±2.16%、35.1±1.40%、34.22±1.67%和31.85±0.75%;IB4染色陽性的神經(jīng)元比率分別為17.77±0.70%、33.22±2.92%、41.36±5.84%、41.38±3.27%、43.83±5.06%和 38.22±4.10%;全部傷害性感覺神經(jīng)元(表達(dá)trkA或被IB4陽性染色細(xì)胞)的比例依次為78.65±2.03%、88.90±2.58%、91.11±6.39%、69.10±3.52%、70.54±5.34%、54.71±3.49%;trkA與IB4結(jié)合蛋白共表達(dá)的神經(jīng)元為數(shù)較少,分別為8.63±1.01%、8.93±1.17%、4.12±0.89%、7.42±1.24%、7.51±1.23%和15.36±0.96%。成年大鼠的背根神經(jīng)節(jié)中,trkA陽性、IB4陽性、trkA或IB4單陽性,以及trkA和IB4雙陽性的比率依次為 30.24±0.27%、39.58±1.03%、54.70±0.87%和 14.60±0.14%。結(jié)論:三叉神經(jīng)節(jié)中,不介導(dǎo)神經(jīng)性疼痛的trkA-Ab陽性神經(jīng)元在生后發(fā)育的過程中逐漸減少,介導(dǎo)神經(jīng)性疼痛的IB4陽性神經(jīng)元?jiǎng)t逐漸增多,這可能會(huì)引起神經(jīng)性疼痛隨年齡增長(zhǎng)而增多;傷害性感覺神經(jīng)元的總數(shù)量隨著年齡的增長(zhǎng)而減少,可能是疼痛敏感性降低的原因。上述各組數(shù)據(jù)在L4-L6 DRGs中保持一致,亦與成年大鼠TRGs中的數(shù)據(jù)相近,說明機(jī)體內(nèi)有復(fù)雜的機(jī)制調(diào)控著發(fā)育進(jìn)程不同的DRG和TRG傷害性感覺神經(jīng)元并使它們的比例維持一致。
[Abstract]:Objective: the sensory ganglion consists of trigeminal ganglia trigeminal ganglion TRG and dorsal root ganglion DRGG, all of which are composed of various types of sensory neurons. Sensory neurons transform specific stimuli into action potentials or graded potentials. Sending sensory information to the brain or spinal cord, including nociceptive sensory neurons responsible for pain perception, which signals tissue damage or response to threats, is an important way for the body to protect itself. There is a lack of reports on the composition and changes of nociceptive sensory neurons in TRG at different developmental ages. Therefore, the purpose of this study was to investigate the changes and regularity of nociceptive sensory neurons in TRG of rats at different developmental ages. The results were compared with those of the nociceptive sensory neurons in rat dorsal root ganglion (DRG). Methods: Sprague-Dawley SD rats of different developmental ages were selected. Adult rats were perfused with the heart after local anesthesia, and then TRGs were taken from the right side of the rat. The DRGs of the adult rats were simultaneously taken from the same side of the back of the same side. All the tissues were immediately immersed in 4% paraformaldehyde for further dehydration. After transparent and wax immersion, paraffin sections were prepared. All nociceptive sensory neurons were stained with anti-tyrosine kinase AantibodytrkA-Aband phytoagglutinin B4 Isolectin-B4IB4. The ratio of all kinds of positive labeled neurons to all sensory neurons in ganglion was calculated, and the quantitative data were misrepresented by mean 鹵standard. Results: from the day of birth to adulthood, there was significant difference between the two groups. The percentage of neurons expressing trkA in trigeminal ganglion was 69.51 鹵2.02and 60.15 鹵1.38and 53.87 鹵2.163.87 鹵1.400.35.1 鹵1.400.75 鹵34.22 鹵1.67% and 31.85 鹵0.7575 respectively. The positive rate of IB4 staining in trigeminal ganglion was 17.77 鹵0.703.22 鹵2.9221.36 鹵5.841.36 鹵5.8441.38 鹵3.27 鹵43.83 鹵5.06% and 38.22 鹵4.10.The percentage of all nociceptive sensory neurons (expressing trkA or IB4 positive cells) was 17.77 鹵0.703.38 鹵3.27 鹵43.83 鹵5.06% and 38.22 鹵4.10respectively. The ratio was 78.65 鹵2.03, 88.90 鹵2.58 and 91.11 鹵6.39. The number of neurons co-expressing IB4 binding protein was less than that of 70.54 鹵5.34 鹵54.71 鹵3.49trkA and 69.10 鹵3.52, respectively, and the ratio of 78.65 鹵2.03 and 88.90 鹵2.58 was 91.11 鹵6.39 and 79.10 鹵3.52 and 54.71 鹵3.49trkA respectively. 8.63 鹵1.01T = 8.93 鹵1.171.17 鹵4.12 鹵0.89 and 7.42 鹵1.24, 7.51 鹵1.23% and 15.36 鹵0.96.The positive rates of trkA and IB4 in dorsal root ganglion of adult rats were 30.24 鹵0.2739.58 鹵1.039.58 鹵1.034.70 鹵0.87% and 14.60 鹵0.140.Conclusion: in the trigeminal ganglion, the positive rate of TRA and IB4 were 30.24 鹵0.2739.58 鹵1.030.70 鹵0.87% and 14.60 鹵0.14.Conclusion: in the trigeminal ganglion, the positive rate of trkA and IB4 is 30.24 鹵0.2739.58 鹵1.039.58 鹵1.034.70 鹵0.87% and 14.60 鹵0.14.4 respectively. The number of trkA-Ab positive neurons that did not mediate neuropathic pain decreased gradually during postnatal development, while the IB4 positive neurons involved in neuropathic pain increased gradually, which may cause neuropathic pain to increase with age. The total number of nociceptive sensory neurons decreased with age, which may be the reason for the decrease in pain sensitivity. These data were consistent in L4-L6 DRGs and similar to those in adult rat TRGs. It is suggested that there are complex mechanisms in the body to regulate the nociceptive neurons of DRG and TRG in different developmental processes and to maintain a consistent proportion of them.
【學(xué)位授予單位】：深圳大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R338

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相關(guān)期刊論文 前1條

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本文編號(hào)：1632537