本文選題：Sonic　切入點：hedgehog　出處：《南開大學(xué)學(xué)報(自然科學(xué)版)》2017年04期 　論文類型：期刊論文

【摘要】：為了揭示下丘腦Sonic hedgehog(Shh)陽性細(xì)胞及其子代細(xì)胞的發(fā)育去向,利用特異性的增強子SBE2(Shh brain enhancer-2,SBE2),構(gòu)建了SBE2-Cre轉(zhuǎn)基因小鼠,并結(jié)合Rosa26-m TmG和Rosa26-LacZ報告基因小鼠,對SBE2+細(xì)胞進(jìn)行了譜系追蹤研究.結(jié)果顯示,小鼠胚胎期E10.5,SBE2+細(xì)胞開始出現(xiàn)在視前區(qū)(preoptic area),然后沿下丘腦前側(cè)區(qū)到下丘腦后側(cè)區(qū)形成條帶,覆蓋了腹側(cè)間腦大部分區(qū)域;胚胎期E12.5,SBE2+細(xì)胞主要出現(xiàn)在視囊,前側(cè)下丘腦,腹側(cè)下丘腦上皮細(xì)胞,乳頭體外側(cè)核,乳頭狀核,外側(cè)下丘腦,在腹側(cè)下丘腦有著較強的表達(dá).胚胎期E16.5和出生后第10 d,SBE2在下丘腦大部分區(qū)域表達(dá).對比內(nèi)源性Shh表達(dá),SBE2+細(xì)胞特異性的代表下丘腦Shh+細(xì)胞,是分化形成下丘腦神經(jīng)核的主要細(xì)胞來源,而SBE2-Cre小鼠可作為研究基因在下丘腦區(qū)域功能的重要工具鼠.
[Abstract]:In order to reveal the developmental direction of the hypothalamic Sonic hedgehog positive cells and their progeny cells, SBE2-Cre transgenic mice were constructed by using the specific enhancer SBE2(Shh brain enhancer-2SSBE2, and combined with Rosa26-m TmG and Rosa26-LacZ reporter gene mice. The lineage of SBE2 cells was studied. The results showed that E10.5 SBE2 cells began to appear in preoptic area at embryonic stage, and then formed bands along the anterior hypothalamus to the posterior hypothalamus, covering most of the ventral diencephalon. E12.5 SBE2 cells mainly appeared in the visual sac, anterior hypothalamus, ventral hypothalamus epithelium, lateral papillary nucleus, papillary nucleus and lateral hypothalamus. There was strong expression in ventral hypothalamus. E16.5 at embryonic stage and SBE2 at 10 days after birth were expressed in most areas of hypothalamus. The specific expression of SBE2 cells in the hypothalamus was compared with that of endogenous Shh cells. SBE2-Cre mouse can be used as an important tool to study the function of gene in hypothalamic region.
【作者單位】： 天津醫(yī)科大學(xué)生物化學(xué)與分子生物學(xué)系;
【基金】：國家自然科學(xué)基金(31371329) 天津市應(yīng)用基礎(chǔ)與前沿技術(shù)研究計劃(13JCYBJC37200) 高等學(xué)校博士學(xué)科點專項科研基金(20131202120011)
【分類號】：Q42

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