本文關(guān)鍵詞： 慢性高原病 膜鐵轉(zhuǎn)運(yùn)蛋白 鐵調(diào)素調(diào)節(jié)蛋白 II型跨膜絲氨酸蛋白酶　出處：《青海大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:本課題通過制備CMS大鼠模型,探討CMS大鼠模型FPN、TMPRSS6及HJV的變化,為揭示CMS鐵代謝相關(guān)因子的改變,豐富CMS鐵代謝的研究內(nèi)容。方法:選取雄性SD大鼠50只,隨機(jī)分成常氧組和低氧組,常氧組于西寧地區(qū)分別飼養(yǎng)(海拔2260m)7d、15d、30d,各8只大鼠;低氧組于低壓氧艙(模擬海拔5000m)分別飼養(yǎng)7d、15d、30d,低氧7d 8只大鼠,低氧15d、30d各9只大鼠。分別于飼養(yǎng)7d、15d、30d測定大鼠外周血血常規(guī),q RT-PCR法測定大鼠肝臟FPN、肝臟m HJV、肝臟TMPRSS6、肌肉s HJV m RNA表達(dá)水平,Western blotting法定肝臟m HJV、肝臟TMPRSS6、肌肉s HJV蛋白水平,IHC法測大鼠肝臟FPN蛋白水平。結(jié)果:(1)大鼠肝臟FPN m RNA表達(dá)水平,低氧7d、低氧15d、低氧30d均高于各常氧對照組(均P0.01);低氧組內(nèi)比較低氧15d、低氧30d均高于低氧7d(均P0.01)。(2)大鼠肝臟FPN蛋白水平,低氧7d和低氧30d均高于各常氧對照組(均P0.01),低氧15d高于常氧15d(P0.05);低氧組內(nèi)比較低氧15d、低氧30d均高于同組7d(均P0.01),低氧30d高于同組15d(P0.05)。(3)大鼠肝臟m HJV m RNA表達(dá)水平,低氧15d、低氧30d均低于各自常氧對照組(均P0.01);低氧組內(nèi)比較低氧15d、低氧30d均低于低氧7d(P0.05及P0.01),低氧30d低于低氧15d(P0.05)。(4)大鼠肌肉s HJV m RNA表達(dá)水平,低氧7d、低氧15d、低氧30d均高于各自常氧對照組(均P0.01)。(5)大鼠肝臟TMPRSS6 m RNA表達(dá)水平,低氧7d、15d、30d均高于各自常氧對照組(均P0.01);低氧組內(nèi)比較低氧30d高于低氧7d及15d(均P0.01)。(6)大鼠肝臟m HJV蛋白表達(dá)水平,低氧7d、15d、30d比各常氧對照組降低(均P0.01),隨缺氧時(shí)間延長m HJV含量逐漸降低。(7)大鼠肌肉s HJV蛋白表達(dá)水平,低氧7d、15d、30d較常氧對照組升高(P0.05),且隨著缺氧時(shí)間延長s HJV表達(dá)逐漸增加。(8)大鼠肝臟TMPRSS6蛋白表達(dá)水平,低氧7d、15d、30d比各常氧對照組升高(均P0.01),隨缺氧時(shí)間延長TMPRSS6表達(dá)逐漸升高。結(jié)論:在高原低壓低氧條件下,FPN、s HJV、TMPRSS6 m RNA和蛋白表達(dá)水平增加,并隨缺氧時(shí)間延長逐漸增加,m HJV m RNA和蛋白表達(dá)水平減低,并隨缺氧時(shí)間延長逐漸降低。FPN、m HJV、s HJV、TMPRSS6參與CMS鐵代謝的調(diào)節(jié),可能是CMS鐵代謝調(diào)節(jié)的重要靶點(diǎn)。
[Abstract]:Objective: to investigate the changes of CMS rat model and HJV in order to reveal the changes of CMS iron metabolism related factors and enrich the research contents of CMS iron metabolism. Methods: 50 male Sprague-Dawley rats were selected. They were randomly divided into normoxic group and hypoxic group. The normoxic group was fed in Xining area (2260mg / 7d / 15d / 30d, n = 8), hypoxia group was fed in a hypobaric oxygen chamber (simulated altitude 5000m) for 7d / 15d / 30d, hypoxia group (n = 8) for 7 days, respectively, and the hypoxia group (n = 8) were fed with hypoxia for 7 days (n = 8) and hypoxia group (n = 8) for 7 days. The expression levels of liver FPNs, liver mHJVs, liver TMPRSS6, muscle s HJV m RNA, liver TMPRSS6, liver TMPRSS6, liver TMPRSS6 and muscle s HJV protein water were determined by qq RT-PCR assay in peripheral blood of rats fed with hypoxia for 15 days and 30 days, respectively. Results the expression of FPN m RNA in the liver of rats was determined by using the method of flat IHC, and the expression of FPN m RNA in the liver of rats was determined. The levels of FPN protein in the liver of rats in hypoxia group were higher than those in normal control group (all P0.01D), and those in hypoxic group were higher than those in hypoxic group (P0.01g / kg) for 7d, 15d and 30d respectively. The expression of m HJV m RNA in the liver of rats in hypoxic group was higher than that in the control group at 15 days after hypoxia for 15 days and 30 days after hypoxia compared with that of the control group for 7 days (all P0.01 and 30 days of hypoxia were higher than that in the same group at 15 days P0.05. 5. 3), and the expression of m HJV m RNA in the liver of rats in hypoxia group was higher than that in the control group at 15 days after hypoxia (all P0.01 and 30 days of hypoxia was higher than that in the same group at 15 days P0.05. 0. 3). After 15 days of hypoxia, 30 days of hypoxia were lower than that of the control group (P 0.01), and the expression of HJV m RNA was lower in hypoxia group than in hypoxia group for 15 days, hypoxia for 30 days, and hypoxia for 30 days lower than that of hypoxia for 15 days (P0.05, P 0.05, P 0.01), and for 30 days, the expression level of HJV m was lower than that in hypoxic group for 15 days, while that in hypoxia group for 30 days was lower than that in hypoxia group for 15 days. The expression level of TMPRSS6 m RNA in the liver of rats exposed to hypoxia for 7 days, 15 days and 30 days was higher than that in the control group (P0.01. 05). The expression of m HJV protein in the liver of rats in hypoxia group was higher than that in hypoxia group for 7 days and 15 days after hypoxia (P 0.01) and the expression of m HJV protein in the liver of hypoxia group was higher than that of normal control group (P 0.01) for 30 days, and the expression level of m HJV protein was higher in hypoxia group for 30 days than that in hypoxia group for 7 days and 15 days. The expression level of s HJV protein in the muscle of rats was decreased after hypoxia for 7 days and 15 days after hypoxia compared with the control group (P0.01 for all, and the content of m HJV decreased gradually with the prolongation of hypoxia time.) the expression level of s HJV protein in the muscle of rats was lower than that of the control group. Compared with the control group, the expression of TMPRSS6 protein in the liver of rats increased gradually with the prolongation of hypoxia time, and the expression of s HJV increased gradually after hypoxia for 7 days and 15 days, 30 days after hypoxia compared with the control group, and the expression of TMPRSS6 protein in the liver of the rats increased gradually with the prolongation of hypoxia. Compared with the control group, the expression of TMPRSS6 increased gradually with the prolongation of hypoxia time. Conclusion: the expression level of TMPRSS6 m RNA and protein in HJV is increased under low pressure and hypoxia condition at high altitude. With the prolongation of hypoxia time, the expression of HJV m RNA and protein decreased gradually, and with the prolongation of hypoxia time, TMPRSS6 was involved in the regulation of iron metabolism of CMS, which may be an important target for the regulation of CMS iron metabolism.
【學(xué)位授予單位】：青海大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R594.3;R-332

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