本文關鍵詞： EPCs 胚胎鼠跖骨 血管生成 實驗模型　出處：《石河子大學》2017年碩士論文　論文類型：學位論文

【摘要】：目的:1.在胚胎鼠跖骨血管生成實驗基礎上,引入外源性的內(nèi)皮祖細胞(EPCs),建立一種新型體外血管生成模型。研究者可通過此實驗模型直接觀察EPCs對胚胎鼠跖骨血管生成的影響,并借助于此實驗模型可以更好地篩選或反應不同實驗目標對EPCs活性的促進或抑制作用。2.探討EPCs聯(lián)合胚胎鼠跖骨血管形成實驗模型中加入的EPCs的最適數(shù)量、模型的最適共培養(yǎng)時間。方法:1.分離、培養(yǎng)及擴增來源于6-8周齡的C57BL/6小鼠骨髓的內(nèi)皮祖細胞(EPCs);2.從孕17.5天C57BL/6小鼠中分離提取胚胎鼠,無菌條件下將胚胎鼠跖骨取出;3.分別將數(shù)量為5×103、1×104、5×104、1×105的EPCs與胚胎鼠跖骨進行時間分別為12天、14天、16天的聯(lián)合培養(yǎng),并設空白對照組;4.CD31對胚胎鼠跖骨周圍芽生管樣結構進行免疫染色;5.用Image-Pro Plus 6.0對免疫染色陽性結果圖像區(qū)域進行量化;6.SPSS 17.0分析處理數(shù)據(jù)并進行統(tǒng)計學分析。結果:1.利用全骨髓貼壁法結合差時貼壁法可有效分離和擴增EPCs細胞。2.EPCs聯(lián)合胚胎鼠跖骨血管生成實驗模型的最適共培養(yǎng)時間為14天。3.EPCs聯(lián)合胚胎鼠跖骨血管生成實驗模型中加入的EPCs相對最適數(shù)量為5×104。結論:初步構建了EPCs聯(lián)合胚胎鼠跖骨血管生成實驗模型,并實現(xiàn)對EPCs活性進行定量檢測。
[Abstract]:Objective to introduce exogenous endothelial progenitor cells (EPCs) on the basis of angiogenesis of fetal rat metatarsal bone. To establish a new in vitro angiogenesis model, the researchers can directly observe the effects of EPCs on the angiogenesis of the metatarsal bone in embryonic mice. With the help of this experimental model, we can better screen or respond to the effects of different experimental targets on the promotion or inhibition of EPCs activity .2.To explore the EP added in the experimental model of vascularization of the metatarsal bone of EPCs combined with embryo mice. The optimum quantity of Cs. Methods: 1. Isolation, culture and amplification of endothelial progenitor cells from the bone marrow of C57BL / 6 mice aged 6-8 weeks. 2. Embryonic mice were isolated and extracted from C57BL / 6 mice on day 17.5 of gestation, and the metatarsal bones of embryonic mice were removed under aseptic condition. 3. EPCs with a quantity of 5 脳 10 ~ 3 ~ 1 脳 10 ~ 4 ~ 1 脳 10 ~ 4 ~ 1 脳 10 ~ 5 and mouse metatarsal bone were co-cultured for 12 ~ 14 days and 16 days, respectively. A blank control group was set up. 4. CD31 was used for immunocytochemical staining of embryonic mouse perimetatarsal sprouts. 5. Image-Pro Plus 6.0 was used to quantify the image area of immunoreactive results. 6.SPSS 17.0 data processing and statistical analysis. Results:. 1. The best co-culture time was 14 days. 3. The best co-culture time of EPCs cells combined with embryonic mouse metatarsal bone angiogenesis was 14 days. The optimal number of EPCs added in the experimental model of Cs combined with fetal mouse metatarsal bone angiogenesis was 5 脳 10 4. Conclusion:. The experimental model of EPCs combined with fetal rat metatarsal bone angiogenesis was preliminarily constructed. The activity of EPCs was detected quantitatively.
【學位授予單位】：石河子大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R-332

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