本文關(guān)鍵詞： 蘇木乙酸乙酯提取物 動(dòng)脈粥樣硬化 白介素-18 白介素-10　出處：《黑龍江中醫(yī)藥大學(xué)》2017年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】：目的:通過(guò)建立動(dòng)脈粥樣硬化模型大鼠,應(yīng)用蘇木乙酸乙酯提取物干預(yù),觀察大鼠腹主動(dòng)脈血管壁病理形態(tài)學(xué)改變及大鼠血清IL-18、IL-10水平及IL-18/IL-10變化,為蘇木乙酸乙酯提取物抗動(dòng)脈粥樣硬化作用提供客觀依據(jù)。方法:將60只SD大鼠隨機(jī)分為6組,分別為:空白對(duì)照組、模型對(duì)照組、蘇木乙酸乙酯提取物低劑量組、蘇木乙酸乙酯提取物中劑量組、蘇木乙酸乙酯提取物高劑量組、辛伐他汀組�？瞻讓�(duì)照組給予普通飼料喂養(yǎng),其余5組行腹腔注射維生素D3+高脂飼料喂養(yǎng)進(jìn)行動(dòng)脈粥樣硬化模型制備。模型復(fù)制成功后,給予空白對(duì)照組、模型對(duì)照組10mg/ml等體積羧甲基纖維素鈉溶液,辛伐他汀組予10mg/ml等體積辛伐他汀溶液,相當(dāng)于辛伐他汀劑量4.2mg/(kg·d),蘇木乙酸乙酯提取物低、中、高劑量組給予10mg/ml等體積低、中、高濃度的蘇木乙酸乙酯提取物溶液,分別相當(dāng)于生藥量0.0575mg/(kg.d)、0.115mg/(kg·d)、0.230mg/(kg.d),每日給藥 1 次,連續(xù)給藥4周。應(yīng)用HE染色法觀察各組大鼠腹主動(dòng)脈病理形態(tài)學(xué)改變,酶聯(lián)免疫吸附法檢測(cè)各組大鼠血清IL-18、IL-10含量變化。結(jié)果:1.與模型對(duì)照組相比,辛伐他汀組及蘇木乙酸乙酯提取物高、中、低劑量組大鼠腹主動(dòng)脈血管壁的病理?yè)p傷程度減輕。2.與空白對(duì)照組相比,模型對(duì)照組、辛伐他汀組、蘇木乙酸乙酯提取物高、中、低劑量組血清IL-18水平升高,差異有統(tǒng)計(jì)學(xué)意義(P0.05);與空白對(duì)照組相比,模型對(duì)照組、辛伐他汀組及蘇木乙酸乙酯提取物高、中、低劑量組血清IL-10水平升高,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。3.與模型對(duì)照組相比,辛伐他汀組及蘇木乙酸乙酯提取物高、中、低劑量組血清IL-18水平降低,血清IL-10水平升高,差異有統(tǒng)計(jì)學(xué)意義(P0.05)4.與辛伐他汀組相比,蘇木乙酸乙酯提取物高、中、低劑量組血清IL-18水平及IL-10水平無(wú)差異(P0.05)。結(jié)論:1.蘇木乙酸乙酯提取物能夠減輕動(dòng)脈粥樣硬化模型大鼠腹主動(dòng)脈血管壁的病理?yè)p傷。2.蘇木乙酸乙酯提取物能夠降低動(dòng)脈粥樣硬化模型大鼠血清IL-18水平,升高血清IL-10水平,從而使血清IL-18/IL-10趨于平衡,維持機(jī)體致炎/抑炎平衡,降低機(jī)體炎癥反應(yīng)活性。
[Abstract]:Objective: to observe the pathomorphological changes of abdominal aorta vascular wall and serum IL-18 of rats by using ethyl acetate extract of hematoxylum to observe the pathomorphology of abdominal aorta in rats. The level of IL-10 and the change of IL-18/IL-10 provided the objective basis for the anti-atherosclerosis effect of ethyl acetate extract. Methods: 60 Sprague-Dawley rats were randomly divided into 6 groups. They were blank control group, model control group, low dose group, middle dose group and high dose group. Simvastatin group. The blank control group was fed with common diet, the other five groups were fed with vitamin D3 high fat diet to prepare the atherosclerosis model. After the model was successfully replicated, the blank control group was given. The model control group was given 10mg / ml sodium carboxymethyl cellulose solution, and the simvastatin group was given 10mg / ml simvastatin solution of the same volume, which was equivalent to the simvastatin dosage of 4.2 mg / kg 路d). The ethyl acetate extract of Sappa chinensis was low, medium and high dose group was given 10mg / ml solution of ethyl acetate extract of low, medium and high concentration. The same amount as 0.0575 mg / kg / kg / kg / kg / kg / d, 0.230 mg / r / kg / kg / d, respectively, was given once a day. The pathomorphology of abdominal aorta of rats in each group was observed by HE staining and serum IL-18 was detected by enzyme-linked immunosorbent assay (Elisa). Results: 1. Compared with the model control group, simvastatin group and hematoxylum ethylacetate extract were higher than those in the model control group. Compared with the blank control group, the model control group, simvastatin group, ethyl sappan acetate extract was high, medium. The serum IL-18 level in the low dose group was higher than that in the control group (P 0.05). Compared with the blank control group, the level of serum IL-10 in simvastatin group, simvastatin group and ethyl acetate group was higher than that in control group, while the level of serum IL-10 in medium and low dose groups was higher than that in control group. Compared with the model control group, simvastatin group and hematoxylum acetate ethyl acetate extract were higher, and the serum IL-18 level was lower in medium and low dose groups. The level of serum IL-10 was higher than that of simvastatin group, the difference was statistically significant (P 0.05). Compared with simvastatin group, the extract of ethyl acetate of hematoxylum was higher than that of simvastatin group. There was no difference in serum IL-18 and IL-10 levels in low dose group (P0.05). ... conclusion:. 1. Ethyl Sappa acetate extract can reduce the pathological damage of abdominal aorta vascular wall in atherosclerotic rats. 2. Ethyl Acetate extract of Sappa acetate can reduce serum IL-18 in atherosclerotic rats. Level. The level of serum IL-10 was increased, so that serum IL-18/IL-10 tended to balance, maintain the balance of inflammation and inhibition of inflammation, and reduce the activity of inflammatory reaction.
【學(xué)位授予單位】：黑龍江中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：R285.5;R-332

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