本文關(guān)鍵詞： 磨損顆粒 小鼠顱骨溶解模型 人工關(guān)節(jié)置換 無菌松動(dòng)　出處：《中國(guó)老年學(xué)雜志》2013年23期 　論文類型：期刊論文

【摘要】：目的探討建立一種新型、簡(jiǎn)單、經(jīng)濟(jì)、易操作的磨損顆粒誘導(dǎo)小鼠顱骨溶解模型,便于無菌性假體松動(dòng)動(dòng)物模型構(gòu)建。方法制備無菌鹽包埋磨損顆粒;實(shí)驗(yàn)分為3組:A組為生理鹽水組;B組為純鹽組;C組為鹽包埋磨損顆粒組;14 d后統(tǒng)一處死動(dòng)物取出小鼠顱骨,進(jìn)行病理學(xué)分析。結(jié)果 (1)HE染色A組和B組無明顯差別,C組骨質(zhì)疏松明顯,骨質(zhì)結(jié)構(gòu)紊亂,骨吸收增加,骨膜及骨組織細(xì)胞數(shù)量明顯增加,骨內(nèi)細(xì)胞數(shù)量(個(gè)/視野)A組(25±3),B組(27±2),C組(45±5);骨膜炎癥細(xì)胞(個(gè)/視野):A組(1 540±368)、B組(1 610±535)、C組(3 478±376)。(2)電鏡下計(jì)算顱頂骨吸收陷窩面積與視野面積的百分比:A組(2.76±0.48)%、B組(2.95±0.63)%,C組(13.33±2.49)%,C組與A組、B組比較,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。(3)TRAP染色中破骨細(xì)胞數(shù)(個(gè)/視野):A組為(3.842 7±1.182 1);B組為(4.026 1±1.372);C組(17.325±4.210 7)。A組與B組相比,無明顯差別(P0.05);C組與A、B組比較:破骨細(xì)胞數(shù)量明顯增加,溶骨效應(yīng)增強(qiáng)(P0.01),TRAP染色與HE染色結(jié)果相符。結(jié)論鹽溶法構(gòu)建磨損顆粒誘導(dǎo)小鼠顱骨溶解模型是一種簡(jiǎn)單、經(jīng)濟(jì)、可行性強(qiáng)的實(shí)驗(yàn)方法,可以為研究人工關(guān)節(jié)無菌性松動(dòng)的提供較為確切、簡(jiǎn)潔的臨床實(shí)驗(yàn)基礎(chǔ),是一種值得倡導(dǎo)的實(shí)驗(yàn)方法。
[Abstract]:Objective to establish a new, simple, economical and easy to operate wear particle model of mouse skull dissolution, which is convenient for the establishment of animal model of aseptic prosthesis loosening. The experiment was divided into 3 groups: normal saline group; Group B was pure salt group; Group C was salt embedded wear particle group. After 14 days, all the animals were killed to remove the cranial bones of the mice for pathological analysis. Results there was no significant difference between group A and group B in osteoporosis, disorder of bone structure and increase of bone resorption. The number of cells in periosteum and bone tissue increased obviously, and the number of cells in bone in group A (25 鹵3) and group B (27 鹵2) and in group C (45 鹵5); Inflammatory cells of periosteum (1 540 鹵3 68%) in group A (1 610 鹵5 35). The percentage of the area of resorption lacuna of the parietal bone and the area of visual field in group C was calculated by electron microscope (2.76 鹵0.48%). Group B (2.95 鹵0.63) and group C (13.33 鹵2.49) were compared with group A and group B (P < 0.05). There was a significant difference in osteoclast number in P 0.05U. P 3 trap staining (P = 3.8427 鹵1.1821) in group A (P < 0.05). The number of osteoclasts in group A was 3.842 7 鹵1.182 1% (P < 0.05). Group B was 4.0261 鹵1.372U; There was no significant difference between group C (17.325 鹵4.210 7) and group B (P 0.05). The number of osteoclasts in group C was significantly higher than that in group A (P 0.01). Conclusion it is a simple, economical and feasible method to construct the model of mouse skull dissolution induced by wear particles by salt solution. It can provide a more precise and succinct clinical experimental basis for the study of aseptic loosening of artificial joints. It is an experimental method worthy of advocacy.
【作者單位】： 安康職業(yè)技術(shù)學(xué)院附屬醫(yī)院;重慶醫(yī)科大學(xué)檢驗(yàn)系;
【分類號(hào)】：R-332
【正文快照】： 人工關(guān)節(jié)置換術(shù)已經(jīng)有100多年的歷史,是治療嚴(yán)重關(guān)節(jié)疾患、重塑關(guān)節(jié)功能的重要手段,是廣大關(guān)節(jié)患者的福音。然而,隨著關(guān)節(jié)置換例數(shù)的增多,假體晚期無菌松動(dòng)的問題日趨嚴(yán)重,二次翻新治療給廣大患者無論是經(jīng)濟(jì)還是精神上帶來嚴(yán)重的創(chuàng)傷〔1〕。因此,任何有效的通過非手術(shù)方式來預(yù)

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