發(fā)布時(shí)間：2018-01-04 21:20

  本文關(guān)鍵詞：逍遙中空栓對(duì)肝郁型大鼠行為學(xué)以及性激素的藥效學(xué)研究　出處：《山西省中醫(yī)藥研究院》2017年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 逍遙散 中空栓 肝郁 行為學(xué)

【摘要】：1.研究目的本實(shí)驗(yàn)旨在從動(dòng)物行為學(xué)以及性激素E2、T、PRL含量?jī)煞矫鎸?duì)逍遙散免煎顆粒劑以及逍遙中空栓進(jìn)行治療效果的對(duì)比,探討在中醫(yī)傳統(tǒng)理論指導(dǎo)下兩種劑型對(duì)肝郁證大鼠的治療作用,為臨床合理使用逍遙散提供支持。2.研究方法用1月齡雌性SD大鼠50只,隨機(jī)分為5組:空白對(duì)照組、肝郁模型組、逍遙散免煎顆粒劑組、逍遙中空栓高劑量組、逍遙中空栓低劑量組,每組10只,通過(guò)肝郁慢性不可預(yù)知的多種應(yīng)激刺激隨機(jī)組合法復(fù)制肝郁模型,21天后每組做陰道涂片連續(xù)5天,與此同時(shí)做行為學(xué)檢測(cè),逍遙中空栓高、低劑量組塞肛納藥;逍遙散免煎顆粒劑組灌胃,連續(xù)給藥10天,給藥結(jié)束后當(dāng)日繼續(xù)做陰道涂片5天,與此同時(shí)做行為學(xué)檢測(cè),結(jié)束后于第二天處死動(dòng)物,取血備用。3.研究?jī)?nèi)容3.1一般狀態(tài)、體重、攝食實(shí)驗(yàn)開(kāi)始后,每天觀察大鼠的一般狀態(tài),大鼠體重增長(zhǎng)情況、攝食量每3天記錄一次。3.2陰道涂片檢測(cè)分別于造模后、給藥后做大鼠陰道涂片檢測(cè),連續(xù)5天,用顯微鏡觀察各大鼠動(dòng)情周期是否紊亂。3.3行為學(xué)檢測(cè)分別于造模后、給藥后做曠場(chǎng)實(shí)驗(yàn)、高架十字迷宮實(shí)驗(yàn)、糖水偏嗜實(shí)驗(yàn),檢驗(yàn)造模是否成功以及給藥是否有效。3.4性激素檢測(cè)將腹主動(dòng)脈所取的血離心,分離血清后,放射免疫分析法測(cè)定血清E2、T、PRL的含量。3.5統(tǒng)計(jì)學(xué)采用SPSS17.0統(tǒng)計(jì)軟件進(jìn)行分析,計(jì)量資料以均數(shù)±標(biāo)準(zhǔn)差表示,組間比較用獨(dú)立樣本t檢驗(yàn),組內(nèi)比較用配對(duì)t檢驗(yàn),定量資料組間比較采用方差分析,P0.05為差異有統(tǒng)計(jì)學(xué)意義。4.結(jié)果4.1造模成功判定:與空白對(duì)照組比較,造模后肝郁模型組、逍遙散免煎顆粒劑組、逍遙中空栓高劑量組、逍遙中空栓低劑量組的一般狀態(tài)、體重、攝食以及行為學(xué)的均值上觀察有顯著變化,經(jīng)t檢驗(yàn)分析,P0.05差異有統(tǒng)計(jì)學(xué)意義,提示造模成功。4.2各組陰道涂片的觀察:造模后空白對(duì)照組5天涂片中動(dòng)情前期、動(dòng)情期、動(dòng)情間期、靜止期規(guī)律交替出現(xiàn),僅有1天鏡下可見(jiàn)典型角化細(xì)胞增多;肝郁模型組鏡下5天中會(huì)有3-5天連續(xù)出現(xiàn)角化細(xì)胞,逍遙散免煎顆粒劑組、逍遙中空栓高劑量組、逍遙中空栓低劑量組給藥后鏡下5天中角化細(xì)胞有規(guī)律出現(xiàn),部分已經(jīng)恢復(fù)正常動(dòng)情周期。4.3通過(guò)對(duì)行為學(xué)數(shù)據(jù)分析發(fā)現(xiàn):曠場(chǎng)實(shí)驗(yàn)中,從外周格、中央格穿越格數(shù)上,逍遙散免煎顆粒劑組、逍遙中空栓高劑量組、逍遙中空栓低劑量組與肝郁模型組相比P0.05,有統(tǒng)計(jì)學(xué)意義;從站立次數(shù)以及理毛修飾次數(shù)上來(lái)說(shuō),逍遙中空栓高劑量組P0.05,有統(tǒng)計(jì)學(xué)意義;高架十字迷宮實(shí)驗(yàn)中,從開(kāi)臂時(shí)間百分比上,逍遙散免煎顆粒劑組、逍遙中空栓高劑量組、逍遙中空栓低劑量組與肝郁模型組相比P0.05,有統(tǒng)計(jì)學(xué)意義;糖水偏嗜實(shí)驗(yàn)中,逍遙散免煎顆粒劑組、逍遙中空栓高劑量組、逍遙中空栓低劑量組糖水偏嗜度與肝郁模型組相比P0.05,有統(tǒng)計(jì)學(xué)意義;其余組間比較經(jīng)統(tǒng)計(jì)學(xué)分析P0.05,認(rèn)為無(wú)差異。4.4通過(guò)對(duì)性激素E2、T、PRL檢測(cè)結(jié)果分析:逍遙散免煎顆粒劑組、逍遙中空栓高劑量組T、PRL與肝郁模型組相比P0.05,有統(tǒng)計(jì)學(xué)意義;逍遙中空栓低劑量組PRL與肝郁模型組相比P0.05,有統(tǒng)計(jì)學(xué)意義;其余組間比較經(jīng)統(tǒng)計(jì)學(xué)分析P0.05,認(rèn)為無(wú)差異。5.結(jié)論5.1通過(guò)對(duì)大鼠進(jìn)行21天肝郁慢性不可預(yù)知的多種應(yīng)激刺激隨機(jī)組合法,可以成功復(fù)制出肝郁證模型。5.2肝郁模型的雌性大鼠一般狀態(tài)差,體重下降,攝食減少,動(dòng)情周期紊亂,曠場(chǎng)實(shí)驗(yàn)中水平和垂直得分降低,高架十字迷宮實(shí)驗(yàn)中開(kāi)臂停留時(shí)間及次數(shù)比例下降,糖水偏嗜度降低。5.3逍遙中空栓可改善肝郁證雌性大鼠的一般狀態(tài)、增加體重、攝食增多,上調(diào)曠場(chǎng)實(shí)驗(yàn)中水平和垂直得分、高架十字迷宮實(shí)驗(yàn)中開(kāi)臂停留時(shí)間及次數(shù)比例、糖水偏嗜程度以及改善紊亂的動(dòng)情周期、升高血清E2、PRL含量、降低血清T含量,與逍遙散免煎顆粒劑比較無(wú)差異,可以作為一種新劑型應(yīng)用于臨床。
[Abstract]:1 . The aim of this study was to compare the therapeutic effects of Xiaoyao Powder and Xiaoyao hollow suppository from the aspects of animal behavior and sex hormone E2 , T and PRL levels . The rats were randomly divided into five groups : blank control group , liver depression model group , Xiaoyao powder free decoction granule group , Xiaoyao hollow suppository high dose group and Xiaoyao hollow suppository low dose group . Compared with the blank control group , there were significant changes in the normal state , body weight , food consumption and the mean value of Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dose group , Xiaoyao hollow suppository high dose group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dose group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dose group , Xiaoyao hollow suppository high dose group , Xiaoyao hollow suppository high dose group , Xiaoyao hollow suppository high dose group , Xiaoyao hollow suppository high dose group , Xiaoyao hollow suppository high dose group , Xiaoyao hollow suppository high dose group , Xiaoyao hollow suppository high dose group , Xiaoyao hollow suppository high dose group , Xiaoyao hollow suppository high dose group , Xiaoyao hollow suppository high dose group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dose group , Xiaoyao hollow suppository high dosage group , the low dose group of Xiaoyao hollow suppository has significant difference compared with the liver depression model group , there is no difference . 4.4 Through analysis of sex hormone E2 , T , PRL detection result : Conclusion : Compared with the liver - qi model group , there is no difference in the time and frequency proportion of open arms , the increase of serum E2 and PRL levels , the decrease of serum T content , the increase of serum E2 and PRL levels , the decrease of serum T content , and the decrease of serum T content .

【學(xué)位授予單位】：山西省中醫(yī)藥研究院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R285.5;R-332

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