本文關(guān)鍵詞：復(fù)方麥芽丸對(duì)PCOS模型大鼠卵巢組織促性腺激素及類固醇激素合成酶表達(dá)的影響　出處：《重慶醫(yī)科大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 復(fù)方麥芽丸 多囊卵巢綜合征 促性腺激素 類固醇激素合成酶

【摘要】：目的:本研究通過(guò)觀察復(fù)方麥芽丸(Compound Malt Pills,CMP)對(duì)多囊卵巢綜合征(polycystic ovary syndrome,PCOS)大鼠動(dòng)情周期變化、卵巢組織形態(tài)學(xué)變化,卵巢組織促卵泡激素(Follicle-stimulating hormone,FSH)、黃體生成素(Luteinizing hormone,LH)、3β-羥甾脫氫酶(3β-hydroxysteroid dehydrogenase,3β-HSD)和細(xì)胞色素P450酶(Cytochrome-P450,CYP450)表達(dá)的影響,探討復(fù)方麥芽丸治療多囊卵巢綜合征的作用機(jī)理,為臨床應(yīng)用提供實(shí)驗(yàn)依據(jù)。方法:選用9日齡SD雌性大鼠60只,隨機(jī)分為正常組(n=10)和造模組(n=50),造模組大鼠頸背部皮下注射丙酸睪酮建立PCOS模型,造模成功的大鼠隨機(jī)分為模型組,達(dá)英-35組和CMP低、中、高劑量組(n=9)。各治療組以相應(yīng)藥物灌胃,正常組及模型組灌以等量的蒸餾水,各組大鼠均連續(xù)灌胃21d。治療結(jié)束后瑞氏染色法觀察大鼠動(dòng)情周期變化,行HE染色觀察大鼠卵巢組織形態(tài),運(yùn)用免疫組化、Western blot、RT-PCR或q PCR法檢測(cè)卵巢組織中FSH、LH、3β-HSD、CYP450蛋白及基因的表達(dá)。結(jié)果:(1)造模成功的大鼠失去規(guī)律的動(dòng)情周期變化,各治療組性周期有不同程度恢復(fù)。(2)模型組大鼠卵巢組織可見(jiàn)較多囊性擴(kuò)張的卵泡,顆粒細(xì)胞層數(shù)減少,各治療組囊性卵泡直徑不同程度減小,顆粒細(xì)胞層增加,黃體數(shù)量增多。(3)模型組LH表達(dá)顯著升高(P0.01),FSH表達(dá)顯著降低(P0.01),復(fù)方麥芽丸各劑量組LH表達(dá)顯著降低(P0.01),FSH表達(dá)顯著升高(P0.01)。(4)模型組3β-HSD表達(dá)顯著升高(P0.01),CYP450表達(dá)顯著降低(P0.01),復(fù)方麥芽丸各治療組3β-HSD表達(dá)顯著降低(P0.01),CYP450表達(dá)顯著升高(P0.01)。結(jié)論:復(fù)方麥芽丸治療PCOS可能是通過(guò)改善動(dòng)情周期紊亂、卵巢多囊樣改變及調(diào)控卵巢組織FSH、LH、3β-HSD、CYP450的表達(dá)實(shí)現(xiàn)的。
[Abstract]:Objective: This study through the observation of compound malt (Compound Malt Pills, CMP pill) on polycystic ovary syndrome (polycystic ovary, syndrome, PCOS) rat estrous cycle, ovarian morphological changes, ovarian follicle stimulating hormone (Follicle-stimulating hormone, FSH), luteinizing hormone (Luteinizing, hormone, LH), 3 beta hydroxysteroid dehydrogenase (3 P -hydroxysteroid dehydrogenase 3 beta -HSD) and cytochrome P450 enzymes (Cytochrome-P450, CYP450) expression, to explore the mechanism of compound malt pill in the treatment of polycystic ovary syndrome, and provide experimental basis for clinical application. Methods: the 9 day old female SD 60 rats were randomly divided into normal group (n=10) and model group (n=50), model group rats subcutaneous injection of testosterone propionate to establish the PCOS model, the successful model of the rats were randomly divided into model group, -35 group and CMP with low and high dose group (n=9) in the treatment. Group with corresponding drugs by gavage, the normal group and model group were fed with the same amount of distilled water, the rats were continuously administered after treatment ended gastric 21d. Wright staining method to observe the estrous cycle changes, morphological observation of rat ovarian tissue by HE staining, immunohistochemical detection of Western, blot, RT-PCR or ovarian tissue Q PCR in FSH, LH, -HSD 3 beta, CYP450 protein expression and gene. Results: (1) the changes of estrous cycle in rats without law, each group of cycles have different degree of recovery. (2) model rats with polycystic ovarian tissue expansion of the follicle reduce the number of granule cells, each treatment group, cystic follicular diameter reduced in different degree, granule cell layer increased, the number of corpus luteum increased. (3) the expression of LH in model group increased significantly (P0.01), the expression of FSH was significantly decreased (P0.01), compound malt pill each dose group LH was significantly decreased, the expression of FSH (P0.01) significant Increased (P0.01). (4) model group 3 beta -HSD expression was significantly increased (P0.01), the expression of CYP450 was significantly decreased (P0.01), malt pill in the treatment group compound 3 beta -HSD expression decreased significantly (P0.01), the expression of CYP450 was significantly increased (P0.01). Conclusion: Compound malt pill in the treatment of PCOS may be improved by estrogen cycle disorder, polycystic ovarian changes and regulation of ovarian tissue FSH, LH, -HSD 3 beta, CYP450 expression.

【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R285.5;R-332

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