老齡貴州小型豬胃黏膜5-HT-IR及SS-IR細胞的初步觀察

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本文關鍵詞：老齡貴州小型豬胃黏膜5-HT-IR及SS-IR細胞的初步觀察　出處：《西北農(nóng)林科技大學學報(自然科學版)》2013年08期 　論文類型：期刊論文

【摘要】：【目的】觀察、比較分析老齡貴州小型豬胃內(nèi)分泌細胞的形態(tài)、數(shù)量和分布�！痉椒ā坎杉淆g和青年貴州小型豬的胃,取胃的不同部位(賁門、胃體、胃竇),用免疫組織化學染色法分別對老齡和青年貴州小型豬胃黏膜中的5-羥色胺(5-HT)、生長抑素(SS)細胞染色,在400倍視野中觀察其形態(tài)及分布,并進行計數(shù),比較老齡與青年貴州小型豬胃黏膜的差異�！窘Y果】老齡和青年貴州小型豬胃黏膜中的5-HT-IR、SS-IR陽性細胞呈錐形、圓形、卵圓形等多種形態(tài);分布不均勻,分別主要分布于胃腺的體部、底部或黏膜表面上皮細胞之間。老齡貴州小型豬胃竇5-HT-IR陽性細胞數(shù)較賁門極顯著增加(P0.01),胃體較賁門顯著增加(P0.05);胃竇SS-IR陽性細胞數(shù)較賁門極顯著增加(P0.01),而胃體與賁門差異不顯著。老齡貴州小型豬5-HT-IR、SS-IR陽性細胞數(shù)較青年貴州小型豬明顯減少,且差異達極顯著水平(P0.01)�！窘Y論】老齡貴州小型豬胃黏膜5-HT-IR、SS-IR陽性細胞的形態(tài)、分布規(guī)律與青年貴州小型豬相似,但分泌5-HT、SS的細胞數(shù)量減少,提示老齡貴州小型豬的胃黏膜內(nèi)分泌功能減退。
[Abstract]:[Objective] to observe and compare the morphology, quantity and distribution of gastric endocrine cells in aged Guizhou miniature pigs. [method] collected in aged and young Guizhou mini pigs in different parts of the stomach, the stomach (cardia, gastric body and antrum), by immunohistochemical staining respectively in aged and young Guizhou mini pigs in gastric mucosa of 5- hydroxytryptamine (5-HT), somatostatin (SS) to observe the morphology and cell staining. The distribution of 400 times in the field of vision, and count, the difference in age and youth of Guizhou miniature pig gastric mucosa. [results] the positive cells of 5-HT-IR and SS-IR in gastric mucosa of old and young Guizhou miniature pigs were cone-shaped, round and oval, and their distribution was uneven. They were mainly distributed in the body, bottom or mucosal surface epithelial cells of gastric glands. The number of 5-HT-IR positive cells in gastric antrum increased significantly (P0.01). The gastric body increased significantly compared with the cardia (P0.05). The number of positive cells in gastric antrum SS-IR was significantly higher than that in gastric cardia (P0.01), but the difference between gastric body and gastric cardia was not significant (P0.01). The number of 5-HT-IR and SS-IR positive cells in aged Guizhou miniature pigs decreased significantly than that of young Guizhou miniature pigs, and the difference was very significant (P0.01). [Conclusion] the morphology and distribution of 5-HT-IR and SS-IR positive cells in gastric mucosa of old Guizhou miniature pigs are similar to those of young Guizhou miniature pigs, but the number of cells secreting 5-HT and SS decreases, suggesting that the endocrine function of gastric mucosa of elderly Guizhou miniature pigs is reduced.
【作者單位】：貴陽中醫(yī)學院實驗動物研究所;貴陽中醫(yī)學院組織學與胚胎學教研室;貴陽中醫(yī)學院基礎醫(yī)學院;
【基金】：“十五”國家科技攻關計劃重點項目(2004BA717B-01-03) 貴州省科技計劃項目(黔科合中藥字[P2012]LKZ7001號)
【分類號】：R-332
【正文快照】： 小型豬作為實驗動物的優(yōu)越性已被越來越多的人所認知,但由于其基礎及應用研究背景資料不足,小型豬還未能取代犬和非人靈長類實驗動物,在生物醫(yī)學研究中充分體現(xiàn)其重要價值。消化道不僅是消化器官,也是體內(nèi)最大、最復雜的內(nèi)分泌器官,其內(nèi)分泌細胞種類繁多,功能各異。消化道內(nèi)

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本文編號：1341054

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