本文選題：煙曲霉菌 + 支氣管上皮細胞�。� 參考：《山東大學學報(醫(yī)學版)》2014年11期

【摘要】：目的探討煙曲霉菌對呼吸道上皮細胞修復功能的影響及其可能機制。方法實驗分為正常對照組、不同濃度(10、20、40 mg/L)AFE組、熱處理AFE組。分別應用不同濃度和熱處理的煙曲霉菌提取物(aspergillus fumigatus extract,AFE)作用體外培養(yǎng)的人支氣管上皮細胞16HBE不同的時間,以細胞體外損傷修復功能、細胞遷移能力、定量細胞黏附能力、細胞伸展面積為指標,觀察AFE和熱處理的AFE對上皮細胞修復能力的影響。結果20、40 mg/L AFE均明顯抑制16HBE細胞的體外損傷修復功能、細胞遷移能力和細胞黏附能力(P0.05),并呈明顯的時間和濃度依賴性。10 mg/L AFE與正常對照組差異無統(tǒng)計學意義(P0.05)。10、20、40 mg/L AFE均明顯抑制細胞的伸展能力(P0.05),并呈明顯濃度依賴性。另外,熱處理AFE的蛋白酶完全滅活,對上述觀察指標無明顯影響(P0.05)。結論 AFE依賴其蛋白酶活性明顯抑制細胞的修復功能,并呈明顯的濃度和時間依賴性。
[Abstract]:Objective to investigate the effect of Aspergillus fumigatus on the repair function of respiratory epithelial cells and its possible mechanism. Methods the experiment was divided into normal control group, AFE group with different concentration (10 ~ 20 mg / L) and heat treated AFE group. Different concentrations and heat treatment of Aspergillus fumigatus extract (aspergillus fumigatus extract AFE) were used to treat 16HBE of human bronchial epithelial cells cultured in vitro for different time. The cell damage repair function, cell migration ability and cell adhesion ability were measured in vitro. The effects of AFE and heat treated AFE on the repair ability of epithelial cells were observed. Results 40 mg / L AFE significantly inhibited the repair function of 16HBE cells in vitro. The cell migration ability and cell adhesion ability (P0.05) were significantly time-and concentration-dependent. 10 mg / L AFE had no significant difference compared with the normal control group (P0.05). 10 mg / L AFE significantly inhibited the cell extension (P0.05), and in a dose-dependent manner. In addition, the protease of heat treatment AFE was completely inactivated, which had no significant effect on the above indexes (P0.05). Conclusion AFE dependent protease activity significantly inhibited the repair function of the cells in a concentration and time dependent manner.
【作者單位】： 山東大學附屬省立醫(yī)院呼吸科;復旦大學附屬華山醫(yī)院呼吸科;
【基金】：山東省自然科學基金(ZR 2010HM104)
【分類號】：R562.25

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