本文選題：肺纖維化 + 間充質(zhì)干細(xì)胞��； 參考：《華中科技大學(xué)》2013年碩士論文

【摘要】：目的：觀察骨髓間充質(zhì)干細(xì)胞（MSCs）移植對(duì)博萊霉素（BLM）誘導(dǎo)的肺纖維化大鼠基質(zhì)金屬蛋白酶及其抑制劑的影響，探討其對(duì)肺纖維化大鼠的作用機(jī)制。 方法：體外分離、培養(yǎng)、純化4周齡SD大鼠的骨髓MSCs。SD實(shí)驗(yàn)大鼠隨機(jī)分為四組（每組12只）：正常對(duì)照組（氣管內(nèi)注入生理鹽水）、模型組（氣管內(nèi)注入BLM）、MSCs治療早期組（BLM造模后立即給予尾靜脈注射MSCs）、MSCs治療晚期組（BLM造模后14天給予尾靜脈注射MSCs），氣管內(nèi)注入BLM用量為5mg／kg，正常對(duì)照組注入等體積生理鹽水，尾靜脈注射MSCs用量為MSCs1.0×106／ml DMEM培養(yǎng)液1ml。于第28天統(tǒng)一處死大鼠后取肺組織，，肺組織病理切片行HE染色及Masson染色觀察肺部炎癥和纖維化情況，蛋白印跡法（Western blot）檢測(cè)肺組織TGF-β1、MMP-2、TIMP-1蛋白表達(dá)量。 結(jié)果：①成功分離培養(yǎng)MSCs并鑒定。②模型組肺泡炎和肺纖維化程度較正常對(duì)照組明顯加重,MSCs治療早期組肺泡炎和肺纖維化程度較模型組顯著減輕, MSCs治療晚期組肺泡炎和肺纖維化程度與模型組比較無(wú)顯著差異。③模型組TGF-β1、TIMP-1蛋白表達(dá)較正常對(duì)照組顯著增加,MSCs治療早期組和MSCs晚期組TGF-β1、TIMP-1蛋白表達(dá)較模型組顯著減少，MMP-2蛋白表達(dá)在各組大鼠間無(wú)顯著差異。 結(jié)論：骨髓間充質(zhì)干細(xì)胞（MSCs）可抑制博萊霉素誘導(dǎo)的肺纖維化并且在肺損傷早期給予MSCs干預(yù)的療效更好，其機(jī)制可能為降低TGF-β1蛋白的表達(dá)，調(diào)節(jié)MMP-2與TIMP-1之間的平衡。
[Abstract]:Aim: to investigate the effects of bone marrow mesenchymal stem cells (MSCs) transplantation on matrix metalloproteinases and their inhibitors in bleomycin (BLM)-induced pulmonary fibrosis rats. Methods: isolated and cultured in vitro, Bone marrow MSCs.SD rats of 4-week-old SD rats were randomly divided into four groups (12 rats in each group): normal control group (normal saline injected into trachea), model group (intratracheal injection of BLM) and early treatment group (BLM). MSCs were injected intravenously into the trachea for 5 mg / kg in the late stage group (14 days after BLM model), and the same volume of normal saline was injected into the normal control group. The dosage of MSCs was 1.0 脳 106/ml DMEM. On the 28th day, the lung tissue was taken out and the lung tissue was stained with HE and Masson staining. The expression of TGF- 尾 1, MMP-2 and TIMP-1 in lung tissue was detected by Western blot. Results MSCs were isolated and cultured successfully and the alveolitis and pulmonary fibrosis degree in model group .2 were significantly aggravated than those in normal control group. The alveolitis and pulmonary fibrosis degree of MSCs in early treatment group was significantly less than that in model group, and that in the late stage of MSCs treatment group was significantly lower than that in the model group. There was no significant difference in the degree of alveolitis and pulmonary fibrosis between the model group and the model group. 3. The expression of TGF- 尾 1 and TIMP-1 protein in the model group was significantly higher than that in the normal control group, and the expression of TGF- 尾 1 and TIMP-1 protein in the early treatment group and the late MSCs group in the model group was significantly lower than that in the model group. There was no significant difference between each group. Conclusion: bone marrow mesenchymal stem cells (MSCs) can inhibit bleomycin-induced pulmonary fibrosis and interfere with MSCs in the early stage of lung injury. The mechanism may be to reduce the expression of TGF- 尾 1 protein and regulate the balance between MMP-2 and TIMP-1.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類(lèi)號(hào)】：R563

【參考文獻(xiàn)】

相關(guān)期刊論文 前3條

1 高麗;馬國(guó)強(qiáng);楊敬平;孫德俊;;轉(zhuǎn)化生長(zhǎng)因子β1和結(jié)締組織生長(zhǎng)因子在大鼠肺纖維化中的表達(dá)[J];臨床肺科雜志;2009年08期

2 畢麗鑫;田鵬娜;王岫崢;王輝;趙磊;賀樹(shù)卿;周斌;;基質(zhì)金屬蛋白酶-9及其抑制劑在COPD合并肺間質(zhì)纖維化大鼠中的作用[J];臨床肺科雜志;2012年03期

3 范延紅;董輝;潘慶;郭文怡;王海昌;;TGF-β1在AVP誘導(dǎo)的心肌成纖維細(xì)胞表型轉(zhuǎn)化中的作用[J];心臟雜志;2012年01期

本文編號(hào)：2081997