本文選題：血吸蟲　切入點(diǎn)：日本　出處：《中國(guó)病原生物學(xué)雜志》2017年01期 　論文類型：期刊論文

【摘要】：目的探討過(guò)繼轉(zhuǎn)移日本血吸蟲感染鼠DC對(duì)IL-13細(xì)胞因子調(diào)控抑制過(guò)敏性哮喘黏液分泌的作用機(jī)制。方法采用MACS磁珠分選方法從日本血吸蟲(SJ)感染小鼠及健康對(duì)照小鼠脾臟中提取DC,分別過(guò)繼轉(zhuǎn)移給BALB/c小鼠,并用OVA誘發(fā)哮喘。4周后處死小鼠,提取肺組織總RNA并制作病理切片,采取qRT-PCR和PAS染色方法等檢測(cè)IL-13mRNA以及蛋白水平。建立DC與CD4+T細(xì)胞共培養(yǎng)體系,檢測(cè)IL-13的表達(dá)情況。結(jié)果肺組織病理學(xué)檢查顯示,與OVA單純哮喘組比較,過(guò)繼轉(zhuǎn)移SJ感染DC組(SJ+OVA)過(guò)敏性哮喘被抑制。qRT-PCR顯示,過(guò)繼轉(zhuǎn)移SJ感染DC組IL-13mRNA表達(dá)受到抑制。PAS染色顯示SJ感染DC組黏液分泌減少,免疫組化檢查該組IL-13的表達(dá)量降低。DC與CD4+T細(xì)胞共培養(yǎng)結(jié)果顯示SEA處理DC能抑制CD4+T細(xì)胞IL-13的表達(dá)。結(jié)論過(guò)繼轉(zhuǎn)移日本血吸蟲感染鼠DC通過(guò)下調(diào)CD4+T細(xì)胞中IL-13細(xì)胞因子的釋放而抑制過(guò)敏性哮喘的黏液分泌。
[Abstract]:Objective to investigate the mechanism of IL-13 cytokines regulating and inhibiting mucus secretion of allergic asthma in adoptive metastatic Schistosoma japonicum infected mice. Methods MACS magnetic beads were used to separate mice infected with Schistosoma japonicum and small healthy controls. DCS was extracted from mouse spleen and transferred to BALB/c mice. The mice were killed after 4 weeks of asthma induced by OVA. The total RNA of lung tissue was extracted and pathological sections were made. The levels of IL-13mRNA and protein were detected by qRT-PCR and PAS staining. The co-culture system of DC and CD4 T cells was established. Results Pulmonary histopathological examination showed that allergic asthma with adoptive SJ infection in DC group was inhibited by .qRT-PCR as compared with OVA asthma group. The inhibition of IL-13mRNA expression in DC infected with adoptive transfer of SJ showed that mucus secretion was decreased in DC infected with SJ. The expression of IL-13 was decreased by immunohistochemistry. The results of co-culture of DC and CD4 T cells showed that DC treated with SEA could inhibit the expression of IL-13 on CD4 T cells. Conclusion DC can down-regulate IL-13 fine in CD4 T cells of mice infected with schistosomiasis japonicum. The release of cytokines inhibits mucus secretion in allergic asthma.
【作者單位】： 天津醫(yī)科大學(xué)病原生物學(xué)系;
【基金】：天津醫(yī)科大學(xué)基金項(xiàng)目(No.2013KY06) 國(guó)家級(jí)市級(jí)大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練項(xiàng)目(No.201410062001)
【分類號(hào)】：R562.25

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