發(fā)布時(shí)間：2018-02-16 09:59

  本文關(guān)鍵詞： 呼吸窘迫綜合征 成人 自噬 肢體缺血再灌注 Beclin LC　出處：《重慶醫(yī)學(xué)》2014年32期 　論文類(lèi)型：期刊論文

【摘要】：目的研究大鼠雙下肢缺血再灌注所致急性肺損傷(LIR-ALI)肺組織細(xì)胞早期自噬及其相關(guān)蛋白的表達(dá)。方法采用LIR-ALI模型,12只雄性SD大鼠,以隨機(jī)數(shù)字表法分為假手術(shù)(Sham)組,肢體缺血再灌注(I/R)組,每組6只。采用ELISA檢測(cè)血清白乳酸脫氫酶(LDH)和免疫熒光法檢測(cè)肺組織病理變化,利用RT-PCR檢測(cè)各組肺組織Beclin1和Atg5mRNA表達(dá)及Western blot檢測(cè)LC3蛋白的表達(dá)。結(jié)果與Sham組比較,I/R組LDH值升高,顯示大鼠LIR-ALI模型造模成功且肺組織免疫熒光特異性標(biāo)記抗體高表達(dá)(P0.01)。采用2-△△CT法分析肺組織Beclin1和Atg5mRNA相對(duì)表達(dá)量,在I/R組較Sham組升高(P0.01);Western blot法檢測(cè)顯示,I/R組較Sham組LC3-Ⅰ、LC3-Ⅱ高表達(dá)、LC3-Ⅱ/GAPDH亦明顯升高(P0.01)。結(jié)論大鼠LIR-ALI后激活肺組織細(xì)胞自噬及其相關(guān)蛋白高表達(dá)。
[Abstract]:Objective to study the expression of early autophagy and its associated proteins in lung tissue of rats with acute lung injury induced by ischemia-reperfusion of lower extremities. Methods Twelve male SD rats with LIR-ALI model were randomly divided into sham operation group. ELISA and immunofluorescence were used to detect the pathological changes of lung tissue in the I / R group, 6 rats in each group, and 6 rats in each group. The serum white lactate dehydrogenase (LDH) and immunofluorescence assay were used to detect the pathological changes of lung tissue. The expression of Beclin1 and Atg5mRNA in lung tissue of each group was detected by RT-PCR and the expression of LC3 protein was detected by Western blot. Results the LDH value of I / R group was higher than that of Sham group. The results showed that the rat model of LIR-ALI was successful and the lung tissue immunofluorescence specific antibody was highly expressed P0.01. The relative expression of Beclin1 and Atg5mRNA in lung tissue was analyzed by 2-CT method. Compared with Sham group, the I / R group was significantly higher than that in Sham group by Western blot assay. The results showed that the higher expression of LC3- 鈪，

本文編號(hào)：1515247