本文關(guān)鍵詞： 結(jié)核性胸膜炎 抗原 細(xì)胞免疫反應(yīng) IFN-γ　出處：《武漢大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2014年01期 　論文類型：期刊論文

【摘要】：目的:探討3種結(jié)核分枝桿菌保守抗原Ag85A、Ag85B和HspX抗原特異性細(xì)胞免疫反應(yīng)對(duì)結(jié)核性胸膜炎的輔助診斷價(jià)值。方法:收集我院收治的胸腔積液患者30例,根據(jù)胸水性質(zhì),分為結(jié)核性胸膜炎組20例,非結(jié)核性胸膜炎組10例。收集胸水樣本,分離胸水樣本中細(xì)胞成分。原核表達(dá)、純化并鑒定結(jié)核分枝桿菌Ag85A、Ag85B、HspX蛋白。用Ag85A、Ag85B及HspX蛋白分別刺激2組患者的胸水細(xì)胞成分,通過ELISA技術(shù)檢測(cè)IFN-γ表達(dá)水平并進(jìn)行組間比較。結(jié)果:Ag85A蛋白刺激后,非結(jié)核性胸膜炎組IFN-γ為(15.50±2.41)ng/L,結(jié)核性胸膜炎組IFN-γ為(1 775.00±362.86)ng/L;Ag85B蛋白刺激后,非結(jié)核性胸膜炎組IFN-γ為(14.89±2.30)ng/L,結(jié)核性胸膜炎組IFN-γ為(2 442.50±418.94)ng/L;HspX蛋白刺激后,非結(jié)核性胸膜炎組IFN-γ為(14.20±2.30)ng/L,結(jié)核性胸膜炎組IFN-γ為(3 570.00±496.41)ng/L;兩組比較均P0.01。結(jié)核性胸膜炎組中,不同患者對(duì)不同抗原刺激所產(chǎn)生的IFN-γ水平有一定差別。結(jié)論:結(jié)核性胸膜炎患者胸水中的細(xì)胞成分經(jīng)Ag85A、Ag85B及HspX蛋白刺激后,IFN-γ水平均不同程度明顯升高,該方法對(duì)結(jié)核性胸膜炎有一定的輔助診斷價(jià)值。
[Abstract]:Objective: to study three conservative antigens of Mycobacterium tuberculosis (Ag85A). The value of Ag85B and HspX antigen-specific cellular immunoreactivity in the diagnosis of tuberculous pleurisy methods: 30 patients with pleural effusion were collected according to the nature of pleural effusion. It was divided into tuberculous pleurisy group (n = 20) and non-tuberculous pleurisy group (n = 10). The samples of pleural effusion were collected, the cell components in pleural effusion samples were isolated, the prokaryotic expression was obtained, and the Ag85A of Mycobacterium tuberculosis was purified and identified. Ag85B HspX protein was used to stimulate the hydrothorax cell components of the two groups with Ag85An Ag85B and HspX protein, respectively. The expression of IFN- 緯 was detected by ELISA and compared among groups. IFN- 緯 was 15.50 鹵2.41 ng / L in non-tuberculous pleurisy group and 1.775.00 鹵362.86 ng / L in tuberculous pleurisy group. After stimulation of Ag85B protein, IFN- 緯 was 14.89 鹵2.30 ng / L in non-tuberculous pleurisy group. IFN- 緯 was 244.2.50 鹵418.94 ng / L in tuberculous pleurisy group. After stimulation of HspX protein, IFN- 緯 was 14.20 鹵2.30 ng / L in non-tuberculous pleurisy group. IFN- 緯 in tuberculous pleurisy group was 3 570.00 鹵496.41 ng / L; In tuberculous pleurisy group. The level of IFN- 緯 produced by different antigen stimulation was different in different patients. Conclusion: the cell components in pleural effusion of tuberculous pleurisy were stimulated by Ag85Agnag85B and HspX protein. The level of IFN- 緯 in tuberculous pleurisy was increased to some extent, and this method was valuable in the diagnosis of tuberculous pleurisy.
【作者單位】： 武漢市結(jié)核病防治所;
【基金】：武漢市衛(wèi)生局資助課題(編號(hào):WG10B03)
【分類號(hào)】：R521.7
【正文快照】： 結(jié)核性胸膜炎是臨床常見的結(jié)核病類型,其診斷方法多樣,如影像學(xué)檢查、胸水性狀觀察、胸水酶及蛋白檢測(cè)、胸膜活檢、結(jié)核分枝桿菌的檢查及培養(yǎng)等。但病原學(xué)及組織病理學(xué)檢查因陽(yáng)性率低、周期長(zhǎng),有創(chuàng)檢查等而難以普及[1,2],所以臨床上往往依靠排他性診斷。隨著免疫學(xué)的進(jìn)展,一些

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本文編號(hào)：1479695