AIM: To optimize the viral persistence rate in a hydrodynamic injection（HI） based hepatitis B virus（HBV） transfection mouse model.METHODS:（1） 5-6-wk-old male C3H/He N and C57BL/6 mice were hydrodynamically injected with 10 μg endotoxin-free p AAV/HBV1.2 plasmid DNA via the tail vein. Hepatitis B surface antigen（HBs Ag）, hepatitis B e antigen（HBe Ag） and HBV DNA, both in the serum and liver, were detected at different time points post HI by ELISA, immunohistochemical staining or quantitative poly... 

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【參考文獻(xiàn)】：
期刊論文
[1]Toll-like receptor 4 plays an anti-HBV role in a murine model of acute hepatitis B virus expression[J]. Ih-Jen Su,Wen-Tsan Chang.  World Journal of Gastroenterology. 2005(42)



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