乙肝病毒感染及其進展的疾病是常見而且影響重大的感染性疾病之一,目前全球約2億4千萬人群為慢性乙肝病毒感染,目前中國不少地區(qū)仍為乙肝高流行區(qū),每年因乙肝感染病情加重死亡的患者多達5萬人。影響乙肝病毒感染因素中,宿主遺傳特性被發(fā)現(xiàn)對乙肝病毒感染及進展有重要影響。近年來發(fā)表的幾個全基因組關聯(lián)研究發(fā)現(xiàn)了位于6號染色體HLA基因家族上的單核苷酸多態(tài)性位點（SN P）與乙肝病毒感染及感染后疾病進展密切相關。HLA基因家族富集了多個免疫相關蛋白的編碼區(qū),我們將通過對這一區(qū)域（主要是HLA-DP/DQ基因簇）的精細定位來發(fā)現(xiàn)更多的影響乙肝病毒感染的基因位點,研究這些位點的作用模式、相互影響以及對感染后疾病進展的影響。方法：我們在本研究中收集了1440例健康入和1648例乙肝感染者的樣本,分別提取DNA后使用zju-iselect定制芯片對HLA基因區(qū)域進行了高密度的SNP位點基因分型精細定位。之后對芯片數(shù)據(jù)結果分別用PLINK軟件和Haploview軟件做單個SNP位點和各連鎖區(qū)塊（linkage block, LD）上單倍型的關聯(lián)分析以及條件關聯(lián)分析,另外還使用R語言程序包haplo.stat s作... 

【文章來源】：浙江大學浙江省 211工程院校 985工程院校 教育部直屬院校

【文章頁數(shù)】：108 頁

【學位級別】：博士

【部分圖文】：

圖３．２?５個連鎖區(qū)塊的ＳＮＰ及單倍型構成圖??＇

浙江大學博±學位論文?正文第一部分結果??注Ａ）與乙肝病毒感染顯著相關的７６個ＳＮＰ位點的散點圖；Ｂ）各連鎖區(qū)挾與基??因位點的關系；Ｃ）由Ｈａｐｌｏｖｉｅｗ軟件繪制的：／６個ＳＮＰ位點在ＨＬＡ－ＤＰ／?ＤＱ基因??簇各連鎖區(qū)扶的結構圖。這些ＳＮＰ在連鎖區(qū)塊內的連鎖強度ＷＤ’表示，具體詳見??附錄圖１。??ｒ－＊ｉＫＩｉｉ?Ｐ＜■歷?Ａ?也??

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