【摘要】：目的探討3種診斷技術(shù)對(duì)結(jié)核病及耐藥結(jié)核病的應(yīng)用價(jià)值。方法使用LED法、Xpert MTB/RIF、固體培養(yǎng)檢測(cè)259例疑似結(jié)核病患者痰標(biāo)本,比較各方法的陽(yáng)性率、靈敏度與特異度;142份菌株使用比例法、Xpert RIF與線性探針檢測(cè)利福平耐藥,比較2種方法與比例法的差異。結(jié)果 259例痰標(biāo)本中,固體培養(yǎng)、LED法、Xpert MTB陽(yáng)性率分別為54.83%、44.79%、55.60%,平均時(shí)間為32.6 d、0.5 h、2.5 h;以固體培養(yǎng)陽(yáng)性的142例為金標(biāo)準(zhǔn),LED法、Xpert MTB陽(yáng)性數(shù)分別為109例、129例,靈敏度為76.76%、90.85%,特異度為94.02%、87.18%。對(duì)142例菌株進(jìn)行藥敏試驗(yàn),利福平耐藥菌株34例,時(shí)間為28 d;以34例為金標(biāo)準(zhǔn),Xpert RIF、線性探針檢測(cè)利福平耐藥符合率分別為94.12%、91.18%,時(shí)間為2.5 h、8 h。結(jié)論 3種檢測(cè)技術(shù)對(duì)結(jié)核病及耐藥結(jié)核病的診斷具有靈敏性高、特異性高、檢測(cè)時(shí)間短等特點(diǎn),在臨床中有很好的應(yīng)用前景與推廣價(jià)值。
[Abstract]:Objective to evaluate the value of three diagnostic techniques in tuberculosis and drug-resistant tuberculosis. Methods sputum samples from 259 suspected tuberculosis patients were detected by LED and Xpert MTB/RIF, solid culture, the positive rate, sensitivity and specificity of each method were compared, and rifampicin resistance was detected by proportional, Xpert RIF and linear probe in 142 strains, and the difference between the two methods and the proportional method was compared. Results in 259 sputum samples, the positive rate of, Xpert MTB by LED method was 54.83%, 44.79%, 55. 60%, the average time was 32. 6 days, 0.5 h, 2.5 h, and the positive number of, Xpert MTB by solid culture was 109 cases, 129 cases, sensitivity was 76. 76%, 90. 85%, specificity was 94. 02%, 87. 18%, the positive rate of, Xpert MTB was 76. 76%, 90. 85%, the specificity was 94. 02%, the specificity was 87. 18%, the positive rate of Led was 76. 76%, 90. 85%, the specificity was 94. 02%, the specificity was 87. 18%. The drug sensitivity test was carried out in 34 cases of rifampicin resistant strains for 28 days, and the coincidence rate of rifampicin resistance detected by gold standard, Xpert RIF, linear probe in 34 cases was 94.12%, 91.8%, and the time was 2.5 h and 8 h, respectively. the coincidence rate of rifampicin resistance was 94.12% and 41.18%, respectively. Conclusion the three detection techniques have the characteristics of high sensitivity, high specificity and short detection time in the diagnosis of tuberculosis and drug-resistant tuberculosis, and have a good application prospect and popularization value in clinic.
【作者單位】： 阜陽(yáng)市疾病預(yù)防控制中心;
【分類號(hào)】：R52
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本文編號(hào)：2522375