【摘要】：目的分析湖北省2009—2015年血吸蟲血清學檢測實驗室能力驗證(PT)結果及存在問題。方法收集2009—2015年湖北省參控實驗室血吸蟲血清學間接血凝試驗(IHA)法及酶聯(lián)免疫吸附測定(ELISA)法能力驗證數(shù)據(jù)資料,并用SPSS 17.0軟件進行統(tǒng)計分析。結果 2009—2015年IHA共檢測了364份樣本,陽性、陰性樣本的符合率分別為82.5%、86.3%,差異無統(tǒng)計學意義(χ~2=0.993,P0.05)。ELISA共檢測626份樣本,陽性、陰性樣本的符合率分別為84.4%、92.9%,差異有統(tǒng)計學意義(χ~2=10.636,P=0.001)。2009開展能力驗證活動后,IHA法僅2013年較2009年樣本符合率提高,差異有統(tǒng)計學意義(χ~2=7.990,P=0.005),而ELISA法樣本檢測符合率雖較2009年有所提高,但差異均無統(tǒng)計學意義(P0.008)。結論參控實驗室血吸蟲血清學檢測能力在2009年后有所提高,但因各種因素影響使檢測能力仍有較大的提升空間。
[Abstract]:Objective to analyze the (PT) results and existing problems in the laboratory of schistosomiasis serological detection in Hubei Province from 2009 to 2015. Methods the data of serological indirect hemagglutination test (IHA) and enzyme-linked immunosorbent assay (Elisa) of Schistosoma japonicum in Hubei ginseng control laboratory from 2009 to 2015 were collected and statistically analyzed by SPSS 17.0 software. Results A total of 364samples were detected by IHA from 2009 to 2015. The coincidence rates of positive and negative samples were 82.5% and 86.3% respectively, the difference was not statistically significant (蠂 ~ 2 鈮，

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