【摘要】：目的探討弓形蟲感染對子一代雄性小鼠發(fā)育期腦部多巴胺水平的影響。方法 36只雌性ICR小鼠隨機(jī)分為健康對照組和弓形蟲感染組,每組18只。感染組每鼠分別經(jīng)口感染弓形蟲PRU弱毒株10個(gè)包囊。感染后90 d,與健康雄性ICR小鼠按1∶1交配。每組各取2只交配成功的母鼠于孕20 d時(shí)剖腹取胎鼠。其他交配成功的母鼠自然分娩。應(yīng)用高效液相色譜-電化學(xué)法對孕20 d的雄性胎鼠及出生后14 d和63 d的雄性仔鼠(各6只)進(jìn)行腦皮層、小腦、海馬和紋狀體多巴胺含量的測定。結(jié)果感染組小鼠感染后死亡3只,余15只感染鼠中,僅6只交配成功。2只于孕20 d剖腹取胎鼠12只,其中雄性7只;4只自然分娩,仔鼠存活21只,其中雄性15只。18只對照組小鼠均交配成功,2只于孕20 d時(shí)剖腹取胎鼠23只,其中雄性12只;16只自然分娩,仔鼠存活179只,其中雄性92只。感染組和對照組雄性胎鼠小腦區(qū)多巴胺含量分別為(413.25±21.78)ng/g和(346.30±51.83)ng/g,感染組顯著高于對照組(P0.01),兩者皮層區(qū)多巴胺含量差異無統(tǒng)計(jì)學(xué)意義(P0.05)。感染組出生后14 d和63 d,雄性仔鼠皮層區(qū)[(462.50±24.80)ng/g和(1 215.77±113.64)ng/g]、小腦區(qū)[(271.55±26.19)ng/g和(1 328.82±39.62)ng/g]、海馬區(qū)[(225.78±24.17)ng/g和(1 322.70±58.34)ng/g]和紋狀體區(qū)[(455.23±61.53)ng/g和(991.32±54.31)ng/g]的多巴胺含量均顯著高于相應(yīng)對照組(P0.05或P0.01)。結(jié)論弓形蟲感染能引起子一代雄性小鼠發(fā)育期腦部多巴胺水平顯著升高。
[Abstract]:Objective to investigate the effect of Toxoplasma gondii (Toxoplasma gondii) infection on dopamine levels in the brain of first generation male mice during development. Methods 36 female ICR mice were randomly divided into healthy control group and Toxoplasma gondii infection group with 18 mice in each group. In the infection group, each mouse was infected with 10 cysts of Toxoplasma gondii PRU attenuated strain. 90 days after infection, the mice were mated with healthy male ICR mice at 1:1. In each group, 2 successfully mated female mice were taken by caesarean section on the 20th day of pregnancy. Other successfully mated female mice give birth naturally. The contents of dopamine in cerebral cortex, cerebellum, hippocampus and striatum of male fetal rats on the 20th day of gestation and 6 on the 14th and 63rd day after birth were determined by high performance liquid chromatography-electrochemical method. Results in the infection group, 3 mice died after infection, and only 6 of the remaining 15 infected mice mated successfully. 12 fetal mice, including 7 males, were taken by laparotomy on the 20th day of gestation. Four mice gave birth naturally, 21 of them survived, of which 15 were males. All the 18 mice in the control group mated successfully, 23 of them were taken by caesarean section on the 20th day of gestation, of which 12 were males, and 179 of them survived naturally, of which 92 were males. The dopamine content in cerebellar area of male fetal rats in infection group and control group was (413.25 鹵21.78) ng/g and (346.30 鹵51.83) ng/g, respectively, which was significantly higher than that in control group (P 0.01). There was no significant difference in dopamine content between the two groups (P 0.05). In the infection group, 14 and 63 days after birth, the cortical area of male offspring [(462.50 鹵24.80) ng/g and (1 215.77 鹵113.64) ng/g], cerebellar area [(271.55 鹵26.19) ng/g and (1 328.82 鹵39.62) ng/g], The dopamine contents in hippocampus [(225.78 鹵24.17) ng/g and (1 322.70 鹵58.34) ng/g] and striatum [(455.23 鹵61.53) ng/g and (991.32 鹵54.31) ng/g] were significantly higher than those in the corresponding control. Group P (P 0.05 or P 0.01). Conclusion Toxoplasma gondii infection can significantly increase the level of dopamine in the brain of male mice during development.
【作者單位】： 江蘇省寄生蟲病防治研究所
【基金】：“十一五”國家科技支撐計(jì)劃重點(diǎn)項(xiàng)目(No.2010BAD04B02) 教育部創(chuàng)新團(tuán)隊(duì)發(fā)展計(jì)劃(No.IRT0978)~~
【分類號】：R531.8

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