【摘要】：目的探索塞來昔布對細(xì)粒棘球蚴原頭節(jié)體外生長及p-ERK2表達(dá)的影響。方法實(shí)驗(yàn)分組為1640培養(yǎng)基組、DMSO組、塞來昔布溶液(50、100、150、200μmol/L)中體外孵育,在倒置焦鏡下觀察原頭節(jié)的形態(tài)變化,同時(shí)通過0.1%伊紅染色顯示原頭節(jié)活力。運(yùn)用Western blot檢測DMSO、100、150、200μmol/L塞來昔布處理細(xì)粒棘球蚴原頭節(jié)48h后p-ERK2蛋白的表達(dá)量。結(jié)果體外孵育7d后,DMSO組及空白1640對照組原頭節(jié)的活力幾乎沒有改變。塞來昔布作用1d后,100、150、200μmol/L組原頭節(jié)的活力均下降;作用3d后,200μmol/L組原頭節(jié)全部被殺死,150μmol/L組的原頭節(jié)活力為43.9%。100μmol/L、50μmol/L組對原頭節(jié)的活力也有明顯下降,但不及高濃度組顯著。Western blot結(jié)果顯示P-ERK2的表達(dá)量隨濃度增加而下降趨勢明顯。結(jié)論塞來昔布能夠抑制細(xì)粒棘球蚴原頭節(jié)生長,這可能與塞來昔布下調(diào)P-ERK2的表達(dá)進(jìn)而抑制ERK2信號傳導(dǎo)通路有關(guān)。
[Abstract]:Objective to investigate the effect of celecoxib on the growth and p-ERK2 expression of Echinococcus granulosus in vitro. Methods the experimental groups were divided into 1640 medium group, DMSO group and celecoxib solution (50100150200 渭 mol / L). The morphological changes of the original head segment were observed under inverted focal microscope, and the activity of the original head segment was shown by 0.1% Ihong staining. The expression of p-ERK2 protein in the original head segment of Echinococcus granulosus treated with DMSO, 100150200 渭 mol / L celecoxib for 48 h was detected by Western blot. Results after incubation in vitro for 7 days, the activity of the original head node in DMSO group and blank 1640 control group was almost unchanged. After celecoxib treatment for 1 day, the activity of the original head decreased in 100150200 渭 mol / L group. After 3 days of treatment, all the primary head segments of 200 渭 mol / L group were killed, and the activity of the original head section of 150 渭 mol / L group was 43.9%. 100 渭 mol / L, and the activity of 50 渭 mol / L group also decreased significantly. However, it was not as significant as that of high concentration group. Western blot results showed that the expression of P-ERK2 decreased significantly with the increase of concentration. Conclusion celecoxib can inhibit the growth of the original head segment of Echinococcus granulosus, which may be related to the down-regulation of P-ERK2 expression by celecoxib and the inhibition of ERK2 signal transduction pathway.
【作者單位】： 石河子大學(xué)醫(yī)學(xué)院第一附屬醫(yī)院肝膽外科;石河子大學(xué)醫(yī)學(xué)院組織胚胎學(xué)教研室;
【基金】：國家自然科學(xué)基金資助項(xiàng)目(No.U1303121)資助~~
【分類號】：R532.32

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