【摘要】：目的:研究不同細(xì)胞因子在登革熱感染患者不同病程中的變化趨勢,為臨床診治提供指導(dǎo);分析細(xì)胞因子在登革熱首次和再次感染中的差異,探討其在登革熱發(fā)病中的作用。方法:根據(jù)ELISA方法檢測的IgG和IgM抗體水平,將收集的298例登革熱患者血清分為首次和再次感染,用流式細(xì)胞術(shù)檢測分析不同登革熱感染患者發(fā)熱后不同時期血清中的23種細(xì)胞因子水平,分析細(xì)胞因子水平在登革病毒感染患者血清中的特點以及臨床意義。結(jié)果:登革熱首次感染和再次感染患者血清中的細(xì)胞因子ENA-78、I-TAC、Mig、TARC、RANTES、MCP-1和IP-10水平在發(fā)熱后不同階段與健康對照比較均有顯著差異(P0.05)。首次感染患者的IL-10、IFN-α和MCP-1水平在發(fā)熱后1-4天(P0.05,P0.01,P0.001)、IFN-γ水平在發(fā)熱后5-6天(P0.05,)、Mig水平在發(fā)熱后5-6(P0.05)天和 7-8 天(P0.05)、TARC 水平在發(fā)熱后 1-4 天(P0.01)和 5-6 天(P0.05)與再次感染比較有顯著差異。結(jié)論:細(xì)胞因子在登革病毒感染后的發(fā)病過程起到重要作用,細(xì)胞因子水平的升高或者降低與疾病發(fā)展密切相關(guān),而IL-10、IFN-α、MCP-1、Mig和TARC在登革熱再次感染患者病情的發(fā)展中發(fā)揮著重要,可能是登革熱再次感染患者病情加重的重要原因,這些細(xì)胞因子將在登革熱疫苗研發(fā)方面有著重要的應(yīng)用前景。
[Abstract]:Aim: to study the change trend of different cytokines in different course of dengue fever infection, to provide guidance for clinical diagnosis and treatment, to analyze the difference of cytokines in the first and second infection of dengue fever, and to explore the role of cytokines in the pathogenesis of dengue fever. Methods: according to the antibody levels of IgG and IgM detected by ELISA, the sera of 298 patients with dengue fever were divided into the first infection and the second infection. The levels of 23 cytokines in serum of patients with different dengue fever were detected by flow cytometry, and the characteristics and clinical significance of cytokine levels in serum of patients with dengue virus infection were analyzed. Results: the levels of cytokine ENA-78,I-TAC,Mig,TARC,RANTES,MCP-1 and IP-10 in serum of patients with first infection and reinfection of dengue fever were significantly different from those of healthy controls at different stages after fever (P0.05). The levels of IL-10,IFN- 偽 and MCP-1 in patients with first infection were 4 days after fever (P 0.05, P 0.01, P 0.001), and the levels of IFN- 緯 were 5 days after fever and 6 days after fever (P 0.05, P 0.05). The level of Mig was significantly different from that of re-infection at 5-6 (P0.05) and 7-8 (P0.05) days after fever (P0.05) and at 4 (P0.01) and 5-6 (P0.05) days after fever and re-infection, respectively. Conclusion: cytokines play an important role in the pathogenesis of dengue virus infection. The increase or decrease of cytokine level is closely related to the development of the disease, while IL-10,IFN- 偽 and MCP-1, are closely related to the development of the disease. Mig and TARC play an important role in the development of re-infection of dengue fever, which may be an important cause of exacerbation of patients with re-infection of dengue fever. These cytokines will have an important application prospect in the research and development of dengue vaccine.
【學(xué)位授予單位】：廣州中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R512.8;R446.6

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