田鼠巴貝蟲隱性感染鼠再感染、免疫抑制或盲傳后的蟲密度消長規(guī)律研究
發(fā)布時間：2019-02-09 17:55
【摘要】：目的觀察田鼠巴貝蟲(Babesia microti)隱性感染期小鼠經(jīng)再感染、免疫抑制或盲傳健康小鼠后的外周血紅細胞內(nèi)蟲體密度的消長規(guī)律。方法取1只感染種鼠的外周血(蟲密度為20%),腹腔接種6周齡雌性BALB/c健康小鼠12只,100μl/只,用隨機數(shù)字表法分為對照組、再感染組、免疫抑制組和盲傳組,每組3只。4組感染小鼠連續(xù)28 d尾部采血,吉氏染色法觀察田鼠巴貝蟲形態(tài)變化,并計算紅細胞染蟲率,構(gòu)建隱性感染小鼠模型。再感染組隱性感染小鼠再次腹腔接種相同劑量的田鼠巴貝蟲感染種鼠血;免疫抑制組隱性感染小鼠腹腔注射地塞米松,0.5 mg/只,連續(xù)注射5 d;盲傳組3只隱性感染小鼠取眼眶血,分別腹腔盲傳接種健康雌性BALB/c小鼠各3只,100μl/只。3組小鼠繼續(xù)尾部采血28 d,鏡下計數(shù)感染紅細胞,計算染蟲率,并觀察田鼠巴貝蟲形態(tài)變化。結(jié)果對照組、再感染組、免疫抑制組和盲傳組小鼠外周血均于感染后第3天查見田鼠巴貝蟲體,第7天紅細胞染蟲率最高,分別為73.2%、78.0%、76.2%及79.0%,隨后染蟲率逐漸下降,至第28天外周血鏡檢陰性,進入隱性感染階段。再感染組小鼠再次感染后28 d內(nèi),每天染蟲率均為0。免疫抑制組小鼠在免疫抑制后第2天查見蟲體,第12天染蟲率再次達到高峰,為65.2%,隨后逐漸下降,至第22天再次進入隱性感染期。盲傳組新感染的9只小鼠在感染后第4天查見蟲體,第9~10天染蟲率達到高峰,為35.0%~39.0%,隨后逐漸下降,至第28天小鼠進入隱性感染階段。各組感染的田鼠巴貝蟲形態(tài)變化基本一致,染蟲初期以小環(huán)狀體居多;染蟲高峰期多見大環(huán)狀體和長絲狀體;有多蟲寄生現(xiàn)象。結(jié)論田鼠巴貝蟲隱性感染期小鼠有帶蟲免疫現(xiàn)象,并可作為傳染源;經(jīng)免疫抑制后蟲體被激化,可出現(xiàn)與首次感染相同的蟲體密度消長規(guī)律。
[Abstract]:Objective to observe the growth and decrease of the body density of erythrocytes in mice with (Babesia microti) recessive infection after reinfection, immunosuppression or blind transmission. Methods Peripheral blood (20% worm density) of one infected rat was inoculated intraperitoneally with 12 healthy female BALB/c mice (100 渭 l / mouse) at the age of 6 weeks. The mice were randomly divided into control group, reinfection group, immunosuppression group and blind transmission group. Three mice in each group were collected blood from the tail of infected mice for 28 days. The morphological changes of Babel beetle in voles were observed and the rate of erythrocyte infection was calculated to establish the model of recessive infection. Mice of recessive infection in reinfection group were intraperitoneally inoculated with the same dose of mice infected with Barb 茅 a vole, and mice in immunosuppressive group were injected dexamethasone intraperitoneally with 0.5 mg/ for 5 days. In the blind transmission group, orbital blood was taken from 3 recessive infected mice and inoculated intraperitoneally with 3 healthy female BALB/c mice (100 渭 l / mouse) respectively. The mice in the 3 groups continued to collect blood from the tail for 28 days. The infected red blood cells were counted under microscope and the rate of infection was calculated. The morphological changes of Barb 茅 e in voles were observed. Results in the control group, reinfection group, immunosuppressive group and blind transmission group, the mice were found in the peripheral blood on the 3rd day after infection. The erythrocyte infecting rate was the highest on the 7th day, 73.2% and 79.0%, respectively, and 78.2% and 79.0% in the control group, reinfection group, immunosuppression group and blind transmission group respectively. Then the infection rate decreased gradually, and the peripheral blood microscopic examination was negative on the 28th day, and entered the stage of recessive infection. Within 28 days after reinfection, the rate of infection was 0. 5%. On the second day after immunosuppression, the worm body was found in the immunosuppression group, and the rate of infection reached a peak of 65.2 on the 12th day, then decreased gradually, and entered the recessive infection stage again on the 22nd day. In the blind transmission group, 9 newly infected mice were detected on the 4th day after infection, and the infection rate reached a peak of 35.039.0 on the 9th day, and then decreased gradually, and the mice entered the stage of recessive infection on the 28th day. The morphological changes of the infected voles in each group were basically the same, most of them were small ring bodies at the initial stage of infection, and the large circular bodies and filamentous bodies were more common in the peak period of infection, and there were many parasites. Conclusion the mice in the recessive infection stage of Barb 茅 b's disease in voles are immune to the parasite and can be used as the source of infection, and the density of the parasite is the same as that of the first infection after the inhibition of immunity.
【作者單位】： 復旦大學生命科學學院;中國疾病預防控制中心寄生蟲病預防控制所世界衛(wèi)生組織熱帶病合作中心科技部國家級熱帶病國際聯(lián)合中心衛(wèi)生部寄生蟲病原與媒介生物學重點實驗室;
【基金】：上海市衛(wèi)計委青年基金項目(No.20154Y0155) 國家科技基礎(chǔ)條件平臺專項寄生蟲病和熱帶病科技資源中心項目 衛(wèi)生公益行業(yè)科研專項(No.201202019)~~
【分類號】：R531
本文編號：2419235
[Abstract]:Objective to observe the growth and decrease of the body density of erythrocytes in mice with (Babesia microti) recessive infection after reinfection, immunosuppression or blind transmission. Methods Peripheral blood (20% worm density) of one infected rat was inoculated intraperitoneally with 12 healthy female BALB/c mice (100 渭 l / mouse) at the age of 6 weeks. The mice were randomly divided into control group, reinfection group, immunosuppression group and blind transmission group. Three mice in each group were collected blood from the tail of infected mice for 28 days. The morphological changes of Babel beetle in voles were observed and the rate of erythrocyte infection was calculated to establish the model of recessive infection. Mice of recessive infection in reinfection group were intraperitoneally inoculated with the same dose of mice infected with Barb 茅 a vole, and mice in immunosuppressive group were injected dexamethasone intraperitoneally with 0.5 mg/ for 5 days. In the blind transmission group, orbital blood was taken from 3 recessive infected mice and inoculated intraperitoneally with 3 healthy female BALB/c mice (100 渭 l / mouse) respectively. The mice in the 3 groups continued to collect blood from the tail for 28 days. The infected red blood cells were counted under microscope and the rate of infection was calculated. The morphological changes of Barb 茅 e in voles were observed. Results in the control group, reinfection group, immunosuppressive group and blind transmission group, the mice were found in the peripheral blood on the 3rd day after infection. The erythrocyte infecting rate was the highest on the 7th day, 73.2% and 79.0%, respectively, and 78.2% and 79.0% in the control group, reinfection group, immunosuppression group and blind transmission group respectively. Then the infection rate decreased gradually, and the peripheral blood microscopic examination was negative on the 28th day, and entered the stage of recessive infection. Within 28 days after reinfection, the rate of infection was 0. 5%. On the second day after immunosuppression, the worm body was found in the immunosuppression group, and the rate of infection reached a peak of 65.2 on the 12th day, then decreased gradually, and entered the recessive infection stage again on the 22nd day. In the blind transmission group, 9 newly infected mice were detected on the 4th day after infection, and the infection rate reached a peak of 35.039.0 on the 9th day, and then decreased gradually, and the mice entered the stage of recessive infection on the 28th day. The morphological changes of the infected voles in each group were basically the same, most of them were small ring bodies at the initial stage of infection, and the large circular bodies and filamentous bodies were more common in the peak period of infection, and there were many parasites. Conclusion the mice in the recessive infection stage of Barb 茅 b's disease in voles are immune to the parasite and can be used as the source of infection, and the density of the parasite is the same as that of the first infection after the inhibition of immunity.
【作者單位】： 復旦大學生命科學學院;中國疾病預防控制中心寄生蟲病預防控制所世界衛(wèi)生組織熱帶病合作中心科技部國家級熱帶病國際聯(lián)合中心衛(wèi)生部寄生蟲病原與媒介生物學重點實驗室;
【基金】：上海市衛(wèi)計委青年基金項目(No.20154Y0155) 國家科技基礎(chǔ)條件平臺專項寄生蟲病和熱帶病科技資源中心項目 衛(wèi)生公益行業(yè)科研專項(No.201202019)~~
【分類號】：R531
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