發(fā)布時間：2018-11-27 12:04

【摘要】：目的分析日本血吸蟲感染以及蟲源性糖蛋白果糖二磷酸醛縮酶(Sj FBPA)刺激后小鼠脾臟細(xì)胞中白介素4(IL-4)、白細(xì)胞分化抗原CD8a因子表達(dá)量的動態(tài)變化,并探討Sj FBPA與輔助型T細(xì)胞(Th2)免疫應(yīng)答的關(guān)系。方法血吸蟲尾蚴感染C57小鼠10只,設(shè)正常對照組。感染8周后處死小鼠,取脾臟制備淋巴細(xì)胞懸液;用藻紅蛋白(PE)標(biāo)記CD8a抗體,藻青蛋白(APC)標(biāo)記IL-4抗體進(jìn)行細(xì)胞染色;流式細(xì)胞儀檢測細(xì)胞因子表達(dá)量的變化。10只正常C57小鼠處死后取脾臟制成淋巴細(xì)胞懸液,加Sj FBPA蛋白體外刺激,按上述流程刺激方法檢測細(xì)胞。結(jié)果小鼠感染血吸蟲8周后脾細(xì)胞中IL-4、CD8a的表達(dá)量分別為(22.39±5.93)%、(11.34±2.15)%,正常對照組脾細(xì)胞中IL-4、CD8a的表達(dá)量分別為(1.39±0.52)%、(1.98±0.84)%,差異有統(tǒng)計學(xué)意義(P0.01)。Sj FBPA體外刺激小鼠脾細(xì)胞的IL-4、CD8a的表達(dá)量分別為(14.14±1.16)%、(8.99±0.81)%,正常對照組脾細(xì)胞中IL-4、CD8a的表達(dá)量分別為(1.86±0.93)%、(1.69±0.56)%,差異有統(tǒng)計學(xué)意義(P0.01)。結(jié)論 Sj FBPA在血吸蟲感染中具有誘導(dǎo)Th2免疫偏移的作用,樹突狀細(xì)胞作為提呈細(xì)胞參與該免疫反應(yīng)。
[Abstract]:Objective to analyze the dynamic changes of expression of interleukin 4 (IL-4) and leukocyte differentiation antigen (CD8a) in spleen cells of mice after Schistosoma japonicum infection and (Sj FBPA) stimulation with insect-derived glycoprotein fructose diphosphate aldolase (Sj FBPA). To investigate the relationship between Sj FBPA and the immune response of helper T cells (Th2). Methods 10 C 57 mice infected with cercariae of Schistosoma japonicum were set up as normal control group. After 8 weeks of infection, the mice were killed and the spleen was taken to prepare lymphocyte suspensions, the CD8a antibody was labeled with phycoerythrin (PE) and the IL-4 antibody was stained with phycocyanin (APC). The expression of cytokines in 10 normal C57 mice was detected by flow cytometry. The spleen of 10 normal C57 mice was taken to make lymphocyte suspension and stimulated by Sj FBPA protein in vitro. Results the expression of IL-4,CD8a in spleen cells was (22.39 鹵5.93)% and (11.34 鹵2.15)% respectively after 8 weeks of schistosomiasis infection in mice, and the expression of IL-4,CD8a in splenocytes of normal control group was (1.39 鹵0.52)%. The expression of IL-4,CD8a was (14.14 鹵1.16)% and (8.99 鹵0.81)%, respectively. The expression of IL-4,CD8a was (1.86 鹵0.93)% and (1.69 鹵0.56)% in the normal control group, respectively. The difference was statistically significant (P0.01). Conclusion Sj FBPA can induce Th2 immune deviation in Schistosoma japonicum infection, and dendritic cells are involved in the immune response as presenting cells.
【作者單位】： 安徽醫(yī)科大學(xué)第一附屬醫(yī)院檢驗(yàn)科;安徽人畜共患病研究所;
【基金】：國家自然科學(xué)基金(編號:81171606)
【分類號】：R532.21

【共引文獻(xiàn)】

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