【摘要】：目的研究查爾酮類化合物(代號:L2H17,簡稱L2)對流感病毒感染小鼠的保護(hù)作用及引起的病毒性肺炎的治療作用。方法將小鼠隨機(jī)分為6組:正常組、模型組、陽性藥奧司他韋(達(dá)菲)組(20 mg/kg)、L2 20、40、80mg/kg劑量組,適應(yīng)性喂養(yǎng)72 h,滴鼻(IN)造模,于造模前24 h給藥,連續(xù)給藥6 d觀察至14 d,統(tǒng)計各組小鼠的中位生存時間、死亡保護(hù)率和生命延長率,觀察L2對流感病毒感染小鼠的保護(hù)作用;按上法于造模后第3、5天無菌條件取小鼠全肺,計算肺指數(shù)及肺指數(shù)抑制率,左肺用4%甲醛固定,用于病理組織切片;右肺浸泡于RNAstore,檢測肺組織病毒載量;小鼠摘眼球取血,采用雙抗體夾心ELISA法檢測小鼠血清中炎癥細(xì)胞因子IL-6、TNF-α含量,觀察L2對流感病毒感染引起的病毒性肺炎的治療作用。結(jié)果與模型組相比,L2 80 mg/kg劑量組死亡率下降50%,中位生存時間、死亡保護(hù)率、生命延長率均有所提高;造模后第3、5天,L2 80 mg/kg劑量組肺指數(shù)降低,病理形態(tài)也有所改善;小鼠肺組織病毒載量降低(P0.05);炎癥因子IL-6含量較模型組有不同程度的降低。結(jié)論L2對流感病毒感染小鼠具有一定的保護(hù)作用,能減輕流感病毒所致肺炎的病變程度。
[Abstract]:Objective to study the protective effect of chalcone compounds (code name: L2H17, L2) on mice infected with influenza virus and its therapeutic effect on viral pneumonia. Methods mice were randomly divided into 6 groups: normal group, model group, positive drug oseltamivir (Tamiflu) group (20 mg/kg), L220 + 400.80mg / kg group. The mice were fed adaptively for 72 h and were given nasal (IN) 24 hours before the model was made. The median survival time, death protection rate and life prolongation rate of each group were counted to observe the protective effect of L2 on mice infected with influenza virus. The whole lungs of the mice were collected according to the aseptic condition on the 3rd day after modeling. The lung index and the inhibition rate of lung index were calculated. The left lung was fixed with 4% formaldehyde for pathological tissue section, and the right lung was immersed in RNAstore, to detect the viral load in the lung tissue. The serum inflammatory cytokines (IL-6,TNF- 偽) in mice were detected by double antibody sandwich ELISA method, and the therapeutic effect of L2 on viral pneumonia caused by influenza virus infection was observed. Results compared with the model group, the mortality of the L280 mg/kg group was decreased by 50%, the median survival time, the death protection rate and the life prolongation rate were increased. The lung index of L280 mg/kg group was decreased and the pathological morphology was improved. The viral load of mouse lung tissue was decreased (P0.05); the content of inflammatory factor IL-6 was decreased in different degree compared with the model group. Conclusion L 2 has protective effect on mice infected with influenza virus and can reduce the severity of pneumonia caused by influenza virus.
【作者單位】： 山東中醫(yī)藥大學(xué)藥學(xué)院;軍事醫(yī)學(xué)科學(xué)院放射與輻射醫(yī)學(xué)研究所;溫州醫(yī)科大學(xué)藥學(xué)院化學(xué)生物學(xué)研究中心;河南中醫(yī)藥大學(xué);
【基金】：國家自然科學(xué)基金重點資助項目(81230089);國家自然科學(xué)基金面上項目(81473184,31270197)
【分類號】：R511.7
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本文編號：2330062