【摘要】：目的探討丙型肝炎病毒樣顆粒(HCVLPs)在亞甲藍(lán)光化學(xué)滅活處理過程中的變化趨勢及其作為亞甲藍(lán)光化學(xué)法滅活HCV效果評價物的可行性。方法以HCV陽性血漿作為平行對照組(HCV RNA載量約6.53 logcopies/m L)(n=6),將HCVLPs用代血漿調(diào)整成合適濃度(HCV RNA定量約為6.74 logcopies/m L)的懸液作為實(shí)驗(yàn)組(n=5),將2組分裝至PVC血袋中,MB+L 1μmol/L進(jìn)行病毒滅活處理,分別于光照處理0、5、10、20、30 min時取樣,用熒光定量PCR技術(shù)檢測病毒核酸載量;同時以細(xì)胞病變法測定HCV模型病毒sindbis病毒的殘余滴度,驗(yàn)證MB+L滅活HCV的效果。結(jié)果模型病毒sindbis的病毒滴度降至檢測限以下(log TCID50/m L≤0.5);MB+L處理過程中,隨著光照處理時間的增加,HCV及HCVLPs的核酸載量明顯下降,分別從6.53與6.74下降至4.51和2.89log copies/m L(P0.05),且2組在不同取樣點(diǎn)的HCV RNA載量之間具有相關(guān)性(R20.98,Significance F0.01)。結(jié)論 HCVLPs能夠反映MB+L滅活處理中HCV RNA動態(tài)變化,或有望成為安全而有效的HCV滅活評價物來監(jiān)控HCV滅活效果。
[Abstract]:Objective to investigate the change trend of hepatitis C virus like particle (HCVLPs) in methylene blue photochemical inactivation and its feasibility as an evaluation of the efficacy of methylene blue photochemical inactivation of HCV. Methods the HCV positive plasma was used as the parallel control group, the (HCV RNA load was about 6.53 logcopies/m L) (/ nm6), and the HCVLPs substitute plasma was adjusted to the appropriate concentration of (HCV RNA (about 6.74 logcopies/m L) as the experimental group (N5). The two groups were divided into PVC blood bag for inactivation of, MB L 1 渭 mol/L. The viral nucleic acid load was detected by fluorescence quantitative PCR technique at 10 ~ 20 ~ 30 min after light treatment. At the same time, the residual titer of HCV model virus sindbis virus was determined by cytopathic method to verify the effect of MB L inactivation of HCV. Results the virus titer of the model virus sindbis decreased below the detection limit (log TCID50/m L 鈮，

本文編號：2304944