陜西省部分地區(qū)人、畜禽戊型肝炎病毒抗體的檢測
發(fā)布時(shí)間：2018-09-08 21:11
【摘要】：戊型肝炎病毒是戊型肝炎的病原體，可引起急性、病毒性肝炎，表現(xiàn)為腹痛、嘔吐、發(fā)熱、厭食、惡心、乏力、并伴有黃疸、尿色深和肝脾腫大，重度肝炎可表現(xiàn)為肝昏迷、鼻出血、消化道出血、皮膚淤斑、急性腦水腫等�；颊呒半[性感染者是該病的主要傳染源。HEV主要通過糞-口途徑傳播，多集中在發(fā)展中國家，在美國、法國、日本、希臘、西班牙、意大利等發(fā)達(dá)國家也有越來越多關(guān)于戊型肝炎的報(bào)道。而我國有2.1億人感染了HEV。因此，戊型肝炎已成為重要的公共健康問題。 禽戊型肝炎病毒是雞的肝炎脾大或大肝大脾病綜合征的主要病原，在世界許多國家廣泛流行，嚴(yán)重影響了養(yǎng)禽業(yè)的健康發(fā)展。為了調(diào)查禽戊型肝炎病毒中國株（CaHEV）抗體在不同動(dòng)物中的分布情況，，本試驗(yàn)利用間接酶聯(lián)免疫吸附法（ELISA），將原核表達(dá)的CaHEV ORF2羧基端268個(gè)氨基酸編碼的重組蛋白和禽戊型肝炎病毒特有的抗原表位模擬肽peptide-2分別作為包被抗原，對陜西省部分地區(qū)人、豬、雞、犬、牛、羊血清和牛奶、羊奶的抗禽HEV ORF2蛋白的抗體進(jìn)行了檢測。結(jié)果表明，人、豬、雞、犬血清和牛奶、羊奶中抗禽HEV ORF2的抗體陽性率分別為10.32%（67/1677）、3.57%（1/28）、2.9%（1/34）、61.6%（53/86）和3.39%（2/58）、0.56%（1/177），用禽HEV特有的抗原表位peptide-2檢測發(fā)現(xiàn)，上述陽性樣品中含有特異性抗禽HEV抗體的陽性率為8.95%（6/67）、0（0/1）、0（0/1）、28.3%（15/53）和50%（1/2）、100%（1/1），牛、羊血清中未發(fā)現(xiàn)陽性樣本。 結(jié)果提示，人、豬、雞、犬、牛和羊都有禽HEV特異性抗體。其中犬的陽性率最高，但對含有禽HEV特異性抗體的血清樣本進(jìn)行RT-nPCR擴(kuò)增禽HEV RNA，沒有擴(kuò)增到目的基因。因此，并不能證明犬感染了禽HEV。本研究通過間接ELISA法對陜西省部分地區(qū)人畜中禽戊型肝炎病毒抗體進(jìn)行了檢測，豐富了禽戊型肝炎病毒的流行病學(xué)資料，為研究禽戊型肝炎病毒的致病性及可能的人畜共患性奠定基礎(chǔ)。
[Abstract]:Hepatitis E virus is the pathogen of hepatitis E, which can cause acute and viral hepatitis. It is characterized by abdominal pain, vomiting, fever, anorexia, nausea, fatigue, accompanied by jaundice, urine color and hepatosplenomegaly. Epistaxis, gastrointestinal bleeding, skin stasis, acute brain edema and so on. Patients and recessive infections are the main source of infection of the disease. HEV is mainly transmitted through faecal-oral routes, mostly concentrated in developing countries, in the United States, France, Japan, Greece, Spain, More and more reports of hepatitis E have been reported in developed countries such as Italy. And 210 million people in China are infected with HEV.. Therefore, hepatitis E has become an important public health problem. Avian hepatitis E virus is the main pathogen of hepatitis spleen or large liver and spleen disease syndrome in chicken. It is widely prevalent in many countries all over the world, which seriously affects the healthy development of poultry industry. In order to investigate the distribution of (CaHEV) antibody against avian hepatitis E virus Chinese strain in different animals, In this study, indirect enzyme-linked immunosorbent assay (ELISA),) was used to encode the recombinant protein encoding 268 amino acids at the carboxyl end of CaHEV ORF2 and the epitope mimic peptide peptide-2 of avian hepatitis E virus as coating antigens. Antibodies against avian HEV ORF2 protein were detected in pig, chicken, dog, cattle, sheep serum and milk and goat milk. The results showed that the positive rates of anti-avian HEV ORF2 in human, pig, chicken, dog serum, milk and goat's milk were 10.32% (67 / 1677), 3.57% (1 / 28), 2.9% (1 / 34), 61.6% (53 / 86) and 3.39% (2 / 58) 0.56% (1 / 177), respectively. The positive rate of the above positive samples was 8.95% (6 / 67) 0 (0 / 1) 0 (0 / 1) or 28.3% (15 / 53) and 50% (1 / 2) 100% (1 / 1) respectively. No positive samples were found in serum of cattle and sheep. The results suggest that human, pig, chicken, dog, cattle and sheep have avian HEV specific antibodies. The positive rate was the highest in dogs, but the target gene was not amplified by RT-nPCR in serum samples containing specific antibodies to avian HEV. Therefore, it does not prove that the dog is infected with avian HEV. In this study, indirect ELISA method was used to detect avian hepatitis E virus antibody in human and livestock in Shaanxi Province, which enriched the epidemiological data of avian hepatitis E virus. To lay a foundation for studying the pathogenicity and possible zoonosis of avian hepatitis E virus.
【學(xué)位授予單位】：西北農(nóng)林科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：S855.3;R512.6
本文編號(hào)：2231710
[Abstract]:Hepatitis E virus is the pathogen of hepatitis E, which can cause acute and viral hepatitis. It is characterized by abdominal pain, vomiting, fever, anorexia, nausea, fatigue, accompanied by jaundice, urine color and hepatosplenomegaly. Epistaxis, gastrointestinal bleeding, skin stasis, acute brain edema and so on. Patients and recessive infections are the main source of infection of the disease. HEV is mainly transmitted through faecal-oral routes, mostly concentrated in developing countries, in the United States, France, Japan, Greece, Spain, More and more reports of hepatitis E have been reported in developed countries such as Italy. And 210 million people in China are infected with HEV.. Therefore, hepatitis E has become an important public health problem. Avian hepatitis E virus is the main pathogen of hepatitis spleen or large liver and spleen disease syndrome in chicken. It is widely prevalent in many countries all over the world, which seriously affects the healthy development of poultry industry. In order to investigate the distribution of (CaHEV) antibody against avian hepatitis E virus Chinese strain in different animals, In this study, indirect enzyme-linked immunosorbent assay (ELISA),) was used to encode the recombinant protein encoding 268 amino acids at the carboxyl end of CaHEV ORF2 and the epitope mimic peptide peptide-2 of avian hepatitis E virus as coating antigens. Antibodies against avian HEV ORF2 protein were detected in pig, chicken, dog, cattle, sheep serum and milk and goat milk. The results showed that the positive rates of anti-avian HEV ORF2 in human, pig, chicken, dog serum, milk and goat's milk were 10.32% (67 / 1677), 3.57% (1 / 28), 2.9% (1 / 34), 61.6% (53 / 86) and 3.39% (2 / 58) 0.56% (1 / 177), respectively. The positive rate of the above positive samples was 8.95% (6 / 67) 0 (0 / 1) 0 (0 / 1) or 28.3% (15 / 53) and 50% (1 / 2) 100% (1 / 1) respectively. No positive samples were found in serum of cattle and sheep. The results suggest that human, pig, chicken, dog, cattle and sheep have avian HEV specific antibodies. The positive rate was the highest in dogs, but the target gene was not amplified by RT-nPCR in serum samples containing specific antibodies to avian HEV. Therefore, it does not prove that the dog is infected with avian HEV. In this study, indirect ELISA method was used to detect avian hepatitis E virus antibody in human and livestock in Shaanxi Province, which enriched the epidemiological data of avian hepatitis E virus. To lay a foundation for studying the pathogenicity and possible zoonosis of avian hepatitis E virus.
【學(xué)位授予單位】：西北農(nóng)林科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：S855.3;R512.6
【參考文獻(xiàn)】
相關(guān)期刊論文 前7條
1 周恩民;荊勝濤;張璐;董施偉;彭軍;孫培明;;禽戊性肝炎研究進(jìn)展[J];山東畜牧獸醫(yī);2008年11期
2 肖愛歡;梁家幸;秦毅斌;趙武;李斌;劉芳;何穎;黃偉堅(jiān);;動(dòng)物戊型肝炎研究進(jìn)展[J];廣西農(nóng)業(yè)科學(xué);2010年02期
3 陳美珠;曾一清;龔方;;人工肝支持治療戊型肝炎的臨床效果[J];齊魯醫(yī)學(xué)雜志;2009年01期
4 樊金萍;李艷萍;宋愛京;李榮成;楊進(jìn)業(yè);王佑春;;廣西地區(qū)野鼠戊型肝炎病毒感染情況調(diào)查[J];微生物學(xué)免疫學(xué)進(jìn)展;2008年04期
5 張璐;周恩民;崔治中;董施偉;荊勝濤;彭軍;孫陪明;孫淑紅;秦卓明;;禽戊型肝炎血清學(xué)調(diào)查以及與肝脾腫大綜合征關(guān)系的研究[J];中國家禽;2008年20期
6 楊德吉,單松華,侯世忠,王亞菊,薄其云,徐福南;部分雞場雞大肝和大脾病的血清學(xué)調(diào)查[J];中國獸醫(yī)科技;1997年06期
7 梁久紅;孟繼鴻;;人、豬、禽戊型肝炎病毒血清學(xué)關(guān)系的研究[J];中華微生物學(xué)和免疫學(xué)雜志;2007年07期
本文編號(hào)：2231710
本文鏈接：http://sikaile.net/yixuelunwen/chuanranbingxuelunwen/2231710.html
最近更新
教材專著
