【摘要】：目的調查新疆阿勒泰山地蜱傳腦炎疫源地特征,分離鑒定蜱傳腦炎病毒。方法通過家畜體表撿法采集寄生蜱;利用間接免疫熒光法檢測當?shù)亟】等巳貉逯序鐐髂X炎病毒IgG抗體;通過將蜱研磨液接種實驗小鼠進行病原動物分離;通過接種BHK-21細胞對蜱傳腦炎病毒進行分離培養(yǎng);利用RT-PCR方法對病毒E蛋白基因片段進行擴增和測序,通過序列分析明確病毒系統(tǒng)進化特征。結果新疆阿勒泰山地白哈巴地區(qū)分布有2種蜱,森林革蜱為優(yōu)勢種(55.6%),其次為邊緣革蜱(44.4%);當?shù)厝巳候鐐髂X炎IgG抗體陽性率5.31%(6/113);通過動物試驗和細胞分離培養(yǎng),從森林革蜱中分離出一株森林腦炎病毒;對病毒E蛋白基因序列的系統(tǒng)進化分析表明,蜱傳腦炎病毒阿勒泰分離株屬于遠東亞型。結論首次從病原學上證實新疆阿勒泰山地存在蜱傳腦炎疫源地,媒介為森林革蜱,病毒流行株為遠東亞型。
[Abstract]:Objective to investigate the characteristics of tick-borne encephalitis foci in Altay Mountain, Xinjiang, and to isolate and identify tick-borne encephalitis virus. Methods the parasitic ticks were collected by the method of animal surface collection, the IgG antibody of tick-borne encephalitis virus was detected by indirect immunofluorescence method, and the pathogenic animals were isolated by inoculating ticks grinding fluid into experimental mice. The tick-borne encephalitis virus was isolated and cultured by inoculating BHK-21 cells, and the viral E protein gene fragment was amplified and sequenced by RT-PCR method, and the phylogenetic characteristics of the virus were identified by sequence analysis. Results there were two species of ticks in Baekhaba area of Altay Mountain, Xinjiang, the dominant species were forest ticks (55.6%), the next were the marginal ticks (44.4%), the positive rate of IgG antibody against tick-borne encephalitis in local population was 5.31% (6 / 113), and the positive rate was 5.31% (6 / 113). A forest encephalitis virus was isolated from the forest ticks, and the phylogenetic analysis of the E protein gene showed that the Acari encephalitis virus Altay isolate belonged to the far East subtype. Conclusion Acari encephalitis foci exist in Altay mountain area of Xinjiang for the first time, the vector is ticks, and the epidemic strain is far East subtype.
【作者單位】： 新疆軍區(qū)疾病預防控制中心;新疆軍區(qū)第十六醫(yī)院;
【基金】：國家自然科學基金項目(No.U1303104)~~
【分類號】：R512.34

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