【摘要】：目的分析吉林省2000~2012年從急性弛緩性麻痹(Acute Flaccid Paralysis,AFP)病例糞便標(biāo)本中分離的脊髓灰質(zhì)炎(脊灰)病毒(Poliovirus,PV)的血清分型以及型內(nèi)鑒別結(jié)果。方法原始糞便標(biāo)本來自吉林省2000~2012年㩳15歲兒童AFP病例,使用轉(zhuǎn)人PV受體的小鼠肺細(xì)胞系和人橫紋肌肉瘤細(xì)胞進(jìn)行病毒分離,陽性標(biāo)本進(jìn)行中和試驗(yàn)鑒定血清型,中和試驗(yàn)鑒定為陽性的PV株送中國疾病預(yù)防控制中心病毒病預(yù)防控制所國家脊灰實(shí)驗(yàn)室進(jìn)行型內(nèi)鑒別,型內(nèi)鑒別方法采用聚合酶鏈反應(yīng)-限制性酶切片段長度多態(tài)性分析和酶聯(lián)免疫吸附試驗(yàn)或VP_1編碼區(qū)核苷酸序列分析法。結(jié)果吉林省2000~2012年共采集804例AFP病例糞便標(biāo)本,通過細(xì)胞培養(yǎng)的方法共有25例分離到PV,均為疫苗相關(guān)(Vaccine-associated)PV(VAPV),其中2008年1例AFP病例分離到Ⅱ型和Ⅲ型VAPV,其中的Ⅲ型PV為VP1編碼區(qū)Ⅲ型和Ⅱ型疫苗重組PV。結(jié)論吉林省2000~2012年未發(fā)現(xiàn)脊灰野病毒或疫苗衍生脊灰病毒引起的AFP病例,為吉林省維持無脊灰提供了實(shí)驗(yàn)室依據(jù)。
[Abstract]:Objective to analyze the serotyping of Poliovirus-PV isolated from fecal specimens of acute flaccid paralysis (Acute Flaccid Paralysisus AFP) cases in Jilin Province from 2000 to 2012. Methods the original fecal specimens were collected from children aged 15 years from 2000 to 2012 in Jilin Province. The virus was isolated from mouse lung cell line and human rhabdomyosarcoma cell line transferred to human PV receptor. The positive specimens were neutralized to identify the serotype. The PV strains identified as positive by neutralization test were sent to the National Polio Laboratory of the Central Center for Disease Control and Prevention of China for in-type identification. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and enzyme-linked immunosorbent assay (Elisa) or nucleotide sequence analysis of VP_1 coding region were used. Results A total of 804 fecal specimens of AFP cases were collected from 2000 to 2012 in Jilin Province. A total of 25 PVs were isolated by cell culture, all of which were vaccine-related. Type 鈪，

本文編號：2161791