唐山市HIV抗體確證試驗(yàn)的替代策略研究
發(fā)布時(shí)間：2018-07-15 21:02
【摘要】：目的在幾種艾滋病病毒(HIV)抗體篩查試驗(yàn)組合中,找出哪種HIV初篩試驗(yàn)組合做蛋白印跡(WB)試驗(yàn)的必要性。方法以2010-2016年,唐山市HIV初篩試驗(yàn)有反應(yīng)的1 972份需進(jìn)一步做WB確證試驗(yàn)的樣本為研究對(duì)象,包括確證結(jié)果為"陽(yáng)性"、"不確定"、"陰性"樣本。兩種快檢(RT)(+)、兩種RT(-)、一種RT(+)或(±),分別與各種酶聯(lián)免疫吸附試驗(yàn)(ELISA)結(jié)果、化學(xué)發(fā)光結(jié)果的各種試驗(yàn)組合與WB結(jié)果及不確定帶型的比較分析。結(jié)果1 972份樣本中,兩種RT(+)與ELISA、化學(xué)發(fā)光的試驗(yàn)組合中,WB確證陽(yáng)性860例,不確定8例,陰性0例;兩種RT(-)的試驗(yàn)組合中,WB不確定137例,2例隨訪后轉(zhuǎn)陽(yáng),陰性925例;一種RT(+)或(±)的試驗(yàn)組合中,WB確證陽(yáng)性7例,陰性20例,不確定15例,其中4例轉(zhuǎn)陽(yáng);三種不同RT組合試驗(yàn)的差異有統(tǒng)計(jì)學(xué)意義(x~2=2 581.367,P=0.001)。在兩種RT(+)與ELISA、化學(xué)發(fā)光的7類(lèi)試驗(yàn)組合與WB結(jié)果的比較無(wú)統(tǒng)計(jì)學(xué)意義(x~2=4.850,P=0.563)。結(jié)論兩種RT(+)兩種ELISA S/C(O值均≥6的試驗(yàn)組合替代WB試驗(yàn)確證HIV感染是完全可行的;兩種RT(一)與一種ELISA陰性的試驗(yàn)組合是沒(méi)必要進(jìn)行WB確證試驗(yàn)的。這種替代策略可以大量降低HIV抗體確證的費(fèi)用,也減少WB不確定結(jié)果帶來(lái)的不必要的傷害。
[Abstract]:Objective to find out which HIV screening test combination is necessary for Western blot (WB) in several HIV antibody screening test combinations. Methods A total of 1 972 samples from Tangshan City from 2010 to 2016 were selected for further WB confirmatory test, including "positive", "uncertain" and "negative" samples. Two kinds of RT) (), RT (-), one RT () or (鹵) were compared with the results of enzyme linked immunosorbent assay (Elisa), chemiluminescence and WB results and uncertain band patterns. Results among the 1 972 samples, 860 cases were confirmed positive, 8 cases were uncertain and 0 cases were negative in two kinds of RT (-) RT (-) and two RT (-) test combinations, 137 cases were confirmed positive, 925 cases were negative after follow up. In one kind of RT () or (鹵) combination, 7 cases were confirmed positive, 20 cases were negative, 15 cases were uncertain, among them, 4 cases changed to positive, and the difference of three different RT combination tests was statistically significant (xan2an2 581.367 P0.001). In the two RT () and ELISAs, there was no significant difference between the seven chemiluminescence test combinations and the WB results. Conclusion it is completely feasible to confirm HIV infection by two RT () two Elisa Sr / C (O 鈮，
本文編號(hào)：2125370
[Abstract]:Objective to find out which HIV screening test combination is necessary for Western blot (WB) in several HIV antibody screening test combinations. Methods A total of 1 972 samples from Tangshan City from 2010 to 2016 were selected for further WB confirmatory test, including "positive", "uncertain" and "negative" samples. Two kinds of RT) (), RT (-), one RT () or (鹵) were compared with the results of enzyme linked immunosorbent assay (Elisa), chemiluminescence and WB results and uncertain band patterns. Results among the 1 972 samples, 860 cases were confirmed positive, 8 cases were uncertain and 0 cases were negative in two kinds of RT (-) RT (-) and two RT (-) test combinations, 137 cases were confirmed positive, 925 cases were negative after follow up. In one kind of RT () or (鹵) combination, 7 cases were confirmed positive, 20 cases were negative, 15 cases were uncertain, among them, 4 cases changed to positive, and the difference of three different RT combination tests was statistically significant (xan2an2 581.367 P0.001). In the two RT () and ELISAs, there was no significant difference between the seven chemiluminescence test combinations and the WB results. Conclusion it is completely feasible to confirm HIV infection by two RT () two Elisa Sr / C (O 鈮，
本文編號(hào)：2125370
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