本文選題：乙型腦炎病毒 + 蚊蟲組成　； 參考：《大理學(xué)院》2013年碩士論文

【摘要】：目的：弄清昭通市流行性乙型腦炎疫情狀況、蚊蟲媒介組成及其優(yōu)勢(shì)蚊蟲帶毒率情況，為當(dāng)?shù)亓餍行砸倚湍X炎的預(yù)防控制提供科學(xué)依據(jù)。 內(nèi)容：收集和分析2005-2011年昭通市流行性乙型腦炎疫情數(shù)據(jù)；現(xiàn)場(chǎng)調(diào)查昭通市蚊蟲組成、夜間活動(dòng)節(jié)律；蚊蟲乙型腦炎病毒帶毒率調(diào)查等。 方法：收集昭通市2005-2011年流行性乙型腦炎疫情數(shù)據(jù)，并進(jìn)行分析；蚊蟲調(diào)查方法，在調(diào)查點(diǎn)畜圈采取誘蚊燈通宵捕獲成蚊，次日對(duì)捕獲的成蚊進(jìn)行分類鑒定并計(jì)數(shù)，以弄清當(dāng)?shù)匚孟x種類組成情況，然后把現(xiàn)場(chǎng)采集的蚊蟲保存于液氮罐帶回實(shí)驗(yàn)室待下一步實(shí)驗(yàn)研宄；同時(shí)采用帳誘法通宵觀察成蚊夜間活動(dòng)習(xí)性調(diào)查；蚊蟲帶毒率調(diào)查，采用細(xì)胞培養(yǎng)法（白紋伊蚊C6/36)進(jìn)行病毒分離，然后提取RNA，最后采用RT-PCR兩步法鑒定病毒并測(cè)序，計(jì)算出蚊蟲自然帶毒率。 結(jié)果：乙腦疫情情況，2005-2011年昭通市乙型腦炎共發(fā)病811例，年均發(fā)病率為2.3820/10萬，其中2006年發(fā)病數(shù)最高；7年間，年齡分布于15歲以下人群發(fā)病較高，占發(fā)病總數(shù)90.01%，7-9月為當(dāng)?shù)匾倚湍X炎發(fā)病高峰。蚊蟲組成情況，共捕獲4屬6亞屬13種114275只成蚊，其中以三帶喙庫蚊、中華按蚊、騷擾阿蚊為主要優(yōu)勢(shì)蚊種，它們的構(gòu)成比分別為85.79%、11.85%和1.44%;通宵蚊蟲調(diào)查發(fā)現(xiàn)，三帶喙庫蚊全夜均有活動(dòng)，，活動(dòng)高峰22:00-23：00。實(shí)驗(yàn)室乙腦病毒檢測(cè)，采用C6/36細(xì)胞接種細(xì)胞分離112組蚊蟲標(biāo)本，其中三帶喙庫蚊（81組）、中華按蚊（22組）、騷擾阿蚊（4組）、致卷庫蚊（5組），結(jié)果陽性25組；經(jīng)采用PCR方法共檢測(cè)出黃病毒陽性標(biāo)本5組，該5組標(biāo)本均鑒定為乙型腦炎病毒，且來自于三帶喙庫蚊；三帶喙庫蚊自然帶毒率為0.28%。；5株乙腦病毒基因型均為基因I型。 結(jié)論：昭通市流行性乙型腦炎流行較嚴(yán)重，蚊蟲種類多，其中三帶喙庫蚊是當(dāng)?shù)貎?yōu)勢(shì)蚊種，也是乙型腦炎主要傳播媒介，自然帶毒率為0.28%。。當(dāng)?shù)匾倚湍X炎流行主要由乙腦病毒基因I型引起，提示當(dāng)?shù)胤酪卟块T應(yīng)做好防蚊滅蚊控制措施，并加強(qiáng)對(duì)易感人群疫苗接種工作。
[Abstract]:Objective: to find out the epidemic situation of epidemic encephalitis B in Zhaotong city, the composition of mosquito vector and its predominant mosquito carrying rate, so as to provide scientific basis for the prevention and control of epidemic encephalitis B in Zhaotong city. Contents: collecting and analyzing epidemic data of epidemic Japanese encephalitis in Zhaotong city from 2005 to 2011; investigation of mosquito composition, night activity rhythm and virus carrying rate of mosquito type B encephalitis virus in Zhaotong City. Methods: the epidemic data of epidemic encephalitis B from 2005 to 2011 in Zhaotong City were collected and analyzed. In order to find out the species composition of the local mosquitoes, and then store the mosquitoes collected in the field in liquid nitrogen tanks for further study; at the same time, observe the nocturnal behavior of adult mosquitoes overnight by using the trap method, and investigate the rate of mosquitoes carrying the virus. The virus was isolated from Aedes albopictus C6 / 36 by cell culture, then RNAs were extracted. The virus was identified by RT-PCR and sequenced. Results: there were 811 cases of Japanese encephalitis in Zhaotong City from 2005 to 2011. The average annual incidence rate was 23.820 / 100 thousand. In 2006, the incidence was the highest in seven years, and the age distribution was higher in the population under 15 years of age. The incidence of Japanese encephalitis was the highest in July and September. Among them, Culex tritaeniorhynchus, Anopheles sinensis and Anopheles albopictus were the dominant species, and their composition ratios were 85.795.85% and 1.44g respectively. Culex tritaeniorhynchus is active all night, with a peak of 22: 00-23: 00. In laboratory, 112 mosquito specimens were isolated by C6 / 36 cell inoculation. Among them, Culex tritaeniorhynchus (81 groups), Anopheles sinensis (22 groups), Anopheles albopictus (4 groups) and Culex humigatus (5 groups) were detected. The results showed that 25 groups were positive. Five groups of yellow virus positive specimens were detected by PCR. All of the five groups were identified as Japanese encephalitis virus and were from Culex tritaeniorhynchus, and the natural carrying rate of Japanese encephalitis virus of Culex tritaeniorhynchus was 0.28. 5 strains of Japanese encephalitis virus were all genotypes of gene I. Conclusion: epidemic encephalitis B is serious in Zhaotong city, and there are many kinds of mosquitoes. Culex tritaeniformis is the dominant mosquito species and the main transmission vector of Japanese encephalitis in Zhaotong city. The epidemic of Japanese encephalitis was mainly caused by Japanese encephalitis gene type I, which suggested that local epidemic prevention departments should do a good job in mosquito control and mosquito control, and strengthen the vaccination of susceptible population.
【學(xué)位授予單位】：大理學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R512.32

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