本文選題：單純皰疹病毒性腦炎 + 血腦屏障��； 參考：《中風與神經(jīng)疾病雜志》2015年10期

【摘要】：目的研究單純皰疹病毒感染小鼠腦組織后MMP-9的變化及對血腦屏障通透性的影響。方法小鼠顱內(nèi)注射單純皰疹病毒1型(HSV-1)制造單純皰疹病毒性腦炎模型,于感染后第3天、第7天,應用明膠酶譜分析檢測腦組織中MMP-9活性,免疫熒光共聚焦檢測MMP-9的細胞來源,通過檢測滲出到腦血管外的伊文斯藍(Evans Blue,EB)的含量以及腦組織水含量觀察血腦屏障的通透性。結果 HSV-1感染后第3天、第7天病毒組中MMP-9活性較正常對照組明顯增高,MMP-9活性增高與血腦屏障通透性增高相一致,給予MMP-9抑制劑BB-94后血腦屏障通透性降低,激活的小膠質(zhì)細胞是MMP-9的主要來源。結論激活的小膠質(zhì)細胞表達MMP-9破壞血腦屏障,在單純皰疹病毒性腦炎的發(fā)病機制中起著重要作用。
[Abstract]:Objective to study the changes of MMP-9 and its effect on blood-brain barrier permeability in mice infected with herpes simplex virus (HSV). Methods Herpes simplex virus encephalitis model was established by intracranial injection of herpes simplex virus type 1 (HSV-1) in mice. On the 3rd and 7th day after infection, the activity of MMP-9 in brain tissue was detected by gelatinase analysis, and the cell origin of MMP-9 was detected by confocal immunofluorescence. The permeability of blood-brain barrier was observed by detecting the content of Evans BlueEB and the water content of brain tissue. Results on the 3rd day after HSV-1 infection, the activity of MMP-9 in the virus group was significantly higher than that in the control group. The increase of MMP-9 activity was consistent with the increase of blood-brain barrier permeability, and the permeability of blood-brain barrier decreased after BB-94, the inhibitor of MMP-9. Activated microglia are the main source of MMP-9. Conclusion the expression of MMP-9 in activated microglia destroys the blood-brain barrier and plays an important role in the pathogenesis of herpes simplex encephalitis.
【作者單位】： 武漢大學人民醫(yī)院神經(jīng)內(nèi)科;
【基金】：中央高校基本科研業(yè)務費專項資金項目(2042015kf0105)
【分類號】：R512.3

【共引文獻】

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本文編號：2062415