本文選題：鐵蛋白 + 轉(zhuǎn)鐵蛋白受體　； 參考：《石河子大學》2014年碩士論文

【摘要】：目的： 通過構(gòu)建結(jié)核分枝桿菌感染小鼠肺泡巨噬細胞模型，探討研究不同毒力的結(jié)核分枝桿菌對小鼠肺泡巨噬細胞鐵代謝調(diào)控作用機制。 方法： 分別將結(jié)核分枝桿菌國際標準強毒株H37Rv菌株（H37Rv）、卡介苗菌株（BCG）以及無菌生理鹽水（空白對照）通過小鼠尾靜脈，，注入小鼠體內(nèi)建立小鼠的感染模型，并在感染后的1d、3d、5d、7d、9d、11d、13d、15d提取小鼠肺泡巨噬細胞總蛋白，利用ELISA方法于模型建立成功后1d、3d、5d、7d、9d、11d、13d、15d檢測各組小鼠肺泡巨噬細胞內(nèi)鐵蛋白、轉(zhuǎn)鐵蛋白受體、膜鐵轉(zhuǎn)運蛋白、乳鐵蛋白的表達，并分析其時相性變化，應用Western blot技術比較分析各組小鼠肺泡巨噬細胞內(nèi)鐵蛋白、轉(zhuǎn)鐵蛋白受體于上述各時間點蛋白表達水平的時相性變化。 結(jié)果： 1.感染組小鼠肺泡巨噬細胞內(nèi)Fn表達量隨時間延長逐漸降低，在感染后7d、9d、11d最低，差異明顯，具有統(tǒng)計學意義（P0.05）。 2.感染導致小鼠肺泡巨噬細胞內(nèi)的TfR表達量逐漸增高，于感染后7d、9d、11d最高，并且H37Rv組高于BCG組表達量，差異具有統(tǒng)計學意義（P0.05）。 3.感染后的小鼠肺泡巨噬細胞內(nèi)FPN的表達量逐漸降低，于感染后5d、7d、9d最明顯，差異具有統(tǒng)計學意義（P0.05）。 4.感染導致小鼠肺泡巨噬細胞分泌較高水平的Lf，與對照組比較在感染后7d、9d、11d升高最明顯，差異均具有統(tǒng)計學意義（P0.05）。 結(jié)論： 1.小鼠肺泡巨噬細胞內(nèi)Fn含量下降，提示感染導致細胞內(nèi)鐵離子含量的下降。 2.小鼠肺泡巨噬細胞巨噬細胞內(nèi)TfR及Lf含量的升高，提示結(jié)核桿菌的感染導致巨噬細胞內(nèi)攝鐵通路的開放。 3.小鼠肺泡巨噬細胞巨噬細胞內(nèi)FPN含量下降，提示結(jié)核桿菌的感染導致巨噬細胞內(nèi)鐵釋放通路的關閉。
[Abstract]:Objective: to study the regulatory mechanism of different virulent mycobacterium tuberculosis on iron metabolism of alveolar macrophages in mice by constructing a mouse alveolar macrophage model infected with Mycobacterium tuberculosis. Methods: Mycobacterium tuberculosis strain H37Rv (International Standard strain H37Rv), Bacillus Calmette-Guerin strain (BCG) and sterile saline (blank control) were injected into the tail vein of mice to establish the model of infection. The total protein of mouse alveolar macrophages was extracted from mice alveolar macrophages at 1 day, 3 days, 5 days, 7 days, 9 days and 9 days after infection. The expression of ferritin, transferrin receptor, membrane ferritin transporter and lactoferrin in alveolar macrophages of each group were detected by Elisa, and the expression of ferritin, transferrin receptor, membrane ferritin transporter and lactoferrin in alveolar macrophages of each group were detected by Elisa, and the expression of ferritin, transferrin receptor, membrane ferritin receptor and lactoferrin in alveolar macrophages of each group were detected by Elisa. The temporal changes of the expression of ferritin and transferrin receptor in alveolar macrophages were analyzed by Western blot. Results: 1. The expression of FN in alveolar macrophages of infected mice decreased gradually with the prolongation of time, and was the lowest at 7 days and 9 days after infection, and the difference was significant (P0.05). The expression of TfR in alveolar macrophages increased gradually after infection, and reached the highest level at day 11d after infection, and the expression of TfR in H37Rv group was higher than that in BCG group (P0.05). 3. The expression of TfR in alveolar macrophages was significantly higher in H37Rv group than that in BCG group (P0.05). The expression of FPN in alveolar macrophages of infected mice decreased gradually, and the expression of FPN in alveolar macrophages was the most obvious on the 5th day and 7th day after infection, and the difference was statistically significant (P0.05). Compared with the control group, the level of Lfsecreted by mouse alveolar macrophages was significantly higher than that of the control group on the 7th day and 9th day after infection, and the difference was statistically significant (P0.05). Conclusion: 1. The content of FN in alveolar macrophages in mice was decreased, indicating that the iron ion content in alveolar macrophages decreased. 2. 2. The increase of TfR and LF contents in alveolar macrophages of mice suggests that the infection of Mycobacterium tuberculosis leads to the opening of TieTong pathway in macrophages. The decrease of FPN content in alveolar macrophages suggested that the iron release pathway in macrophages was shut down due to the infection of Mycobacterium tuberculosis.
【學位授予單位】：石河子大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R52

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相關期刊論文 前3條

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