本文選題：IL-18 + 細胞因子��； 參考：《福建醫(yī)科大學》2014年碩士論文

【摘要】：目的：探討IL-18基因多態(tài)性與中國福建莆田丙型肝炎高發(fā)流行區(qū)漢族人群丙型肝炎病毒的感染易感性；篩選與福建莆田HCV感染高發(fā)流行區(qū)漢族人HCV易感性相關的IL-18等位基因、基因型或單倍型。 方法：采用病例-對照研究設計，收集中國福建莆田地區(qū)漢族人員377人，，其中①丙型病毒性肝炎患者（抗-HCV抗體與HCV-RNA均為陽性）186例；②對照組（抗-HCV抗體陰性）191例，兩組之間根據性別、年齡進行匹配。采用TaqMan-MGB探針方法對IL-18SNPs位點進行多態(tài)性檢測，初步探討IL-18基因多態(tài)性與丙型肝炎易感性的關系。采用多元Logistic回歸模型計算IL-18各基因位點不同基因型人群患丙型肝炎的風險（Odds Ratio，OR）及其95%可信區(qū)間（Confidence Interval，CI）。應用HaploView軟件對IL-18各基因位點進行連鎖不平衡及單倍體分析。 結果：⑴對照人群Hardy-Weinbery平衡檢驗顯示：IL-18-607TG，-137GC，+105AC3個SNP多態(tài)性位點等位基因及基因型頻率分布符合Hardy-Weinbery遺傳平衡（P0.05），說明所選人群具有代表性。 ⑵IL-18各基因位點等位基因頻率分布顯示：-607TG位點的等位基因T和G分布頻率在兩組間基本一致（52.96%，53.40%；47.04%，46.60%）；-137GC位點的等位基因G和C分布頻率在病例組分別為83.60%和16.40%，對照組分別為85.60%和14.40%；+105AC位點的等位基因C和A分布頻率在兩組間無明顯變化（15.86%，15.18%；84.14%，84.82%）。結果顯示IL-18基因-607TG、-137GC、+105AC位點等位基因在病例組和對照組的分布均無統(tǒng)計學意義（P0.05）。⑶各位點基因型與丙型肝炎易感性關系的logistic回歸分析IL-18各基因位點基因型與丙型肝炎易感性關系分析尚未發(fā)現-607TG，-137GC，+105AC位點的基因多態(tài)性與丙型肝炎的易感性存在明顯關聯；⑷IL-18各基因位點的連鎖不平衡分析顯示IL-18各基因位點的連鎖不平衡分析顯示+105AC、-137GC位點存在連鎖不平衡。但病例組與對照組之間單倍型（+105/-137/-607)頻率分布無統(tǒng)計學意義(P0.05)；⑸IL-18基因多態(tài)性基與HCV RNA載量相關性分析HCV-RNA載量與IL-18基因+105AC，-607GT、-137GC位點的基因型均無統(tǒng)計學關聯(P0.05)。 結論：本研究結果顯示：IL-18+105AC，-607GT、-137GC位點基因多態(tài)性與中國福建莆田丙型肝炎高發(fā)流行區(qū)漢族人群HCV感染易感性無明顯關聯。該地區(qū)HCV高度流行的確切機制有待進一步探討。
[Abstract]:Objective: to investigate the relationship between the polymorphism of IL-18 gene and the susceptibility to hepatitis C virus infection in the Han population in Putian, Fujian Province, China, and to screen the IL-18 allele associated with HCV infection susceptibility in the Han nationality in Putian, Fujian Province. Genotype or haplotype. Methods: a case-control study was used to collect 377 Han people from Putian, Fujian, China. Among these 377 Han people, there were 1 hepatitis C patients (both anti-HCV antibody and HCV-RNA were positive in 186 cases) and 2 controls (191 cases were negative for anti-HCV antibody). The two groups were matched according to gender and age. The polymorphism of IL-18 SNPs was detected by TaqMan-MGB probe method, and the relationship between IL-18 gene polymorphism and susceptibility to hepatitis C was preliminarily investigated. Multivariate logistic regression model was used to calculate the risk of hepatitis C infection in different genotypes of IL-18 loci and 95% confidence interval (CI). The linkage disequilibrium and haploid analysis of IL-18 loci were performed with HaploView software. Results the Hardy-Weinbery balance test showed that the alleles and genotype frequencies of the three SNP polymorphism loci in the population of 1: 1 were in accordance with the Hardy-Weinbery genetic balance (P0.05), indicating that the selected population was representative of the alleles of each locus of IL-18, and the alleles of 105AC3 SNP polymorphic loci were in accordance with the Hardy-Weinbery genetic balance (P0.05). The gene frequency distribution showed that the allele T and G of the 1: 607TG locus were basically the same between the two groups (52.96) and 53.40% (P < 0.05). The frequencies of alleles G and C in the case group were 83.60% and 16.40%, respectively, and those in the control group were 85.60% and 14.40, respectively, while the frequencies of allele C and A at 105AC locus had no significant change between the two groups. The results showed that the distribution of alleles of IL-18 gene -607TGf-137GC, 105AC locus in the case group and the control group were not statistically significant. The logistic regression analysis of the relationship between the genotype of IL-18 gene locus and the susceptibility to hepatitis C was made by the logistic regression analysis of the relationship between the genotype of IL-18 gene locus and the type C liver. The relationship between the susceptibility of hepatitis C and the gene polymorphism at the 105AC locus was not found to be significantly associated with the susceptibility of hepatitis C. 4 Linkage disequilibrium analysis of IL-18 loci showed that there was linkage disequilibrium at 105AC-137GC locus. But there was no significant difference in the frequency distribution of the haplotype (105 / -137 / -607) between the case group and the control group. There was no significant correlation between HCV RNA load and HCV-RNA load and the genotype of IL-18 gene 105AC-607GT-137GC. There was no significant correlation between the genotype of HCV-RNA and the genotype of IL-18 gene (105AC-607GT-137GC). Conclusion: the results of this study showed that there was no significant association between the polymorphism of the 1: IL-18 105AC-607GT-137GC locus and the susceptibility to HCV infection in the Han population in Putian, Fujian Province. The exact mechanism of high prevalence of HCV in this area needs to be further explored.
【學位授予單位】：福建醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R512.63

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